REFLEXO DO SERVIÇO NOTURNO FRENTE ÀS CONDIÇÕES DE TRABALHO, SAÚDE, VIDA SOCIAL E FAMILIAR DO PROFISSIONAL DE ENFERMAGEM

O estudo em tela teve como objetivo identificar os resultados das condições de trabalho noturno frente à saúde, vida social e familiar do profissional de Enfermagem. Trata-se de revisão integrativa, com abordagem quantitativa, com a questão de pesquisa: Quais os resultados das condições de trabalho noturno frente à saúde, vida social e familiar do profissional de Enfermagem? A busca foi realizada na Biblioteca Virtual de Saúde, nas bases de dados: LILACS, BDENF, com o resultado de 06 artigos. Na discussão, foi apontada a necessidade de refletir frente aos resultados das condições de trabalho noturno na saúde, vida social e familiar do profissional de Enfermagem. Ao concluir, ficou evidente que os transtornos na vida social e familiar e os agravos à saúde dos membros da equipe de Enfermagem são evitáveis a partir do momento que haja adequações no ambiente laboral, os direitos trabalhistas sejam respeitados, como também a aprovação das lutas da profissão de Enfermagem, fica claro que o bem estar físico e psicológico será preservado.Palavras-chave: Trabalho Noturno; Enfermagem do Trabalho; Saúde do Trabalhador

Influência do sistema complemento na infecção, ativação celular e alteração da permeabilidade endotelial na dengue

Made available in DSpace on 2016-04-12T12:46:44Z (GMT). No. of bitstreams: 2 cintia_marinho_ioc_dout_2015.pdf: 2296721 bytes, checksum: 160c0c4d020b49fe043c76c8f20136ad (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilO Sistema Complemento (SC) desempenha papel importante no controle de infecções atuando na eliminação do patógeno e na regulação da resposta imune. Contudo, uma ativação desregulada do SC gera efeitos deletérios ao hospedeiro, contribuindo para a patogênese de diversas doenças, como na Dengue. Entretanto, o envolvimento do SC na infecção pelo vírus Dengue (DENV) ainda tem vários aspectos a serem investigados. Assim, avaliamos a contribuição do SC na infecção in vitro de monócitos pelo DENV; o perfil de expressão dos Receptores de Complemento (CR) CR1, CR2, CR3, CR4, CD46, CD55 e CD59, e de moléculas de ativação nos monócitos e linfócitos T circulantes de pacientes infectados pelos DENV-1,-2 ou -4 por citometria de fluxo. Dosamos os níveis de SC5b-9 e citocinas em pacientes por ELISA. Avaliamos ainda, a contribuição da ativação do SC na permeabilidade e viabilidade endotelial, utilizando modelo in vitro de células endoteliais (CEs) pela medida da resistência elétrica transendotelial (TEER) e liberação de LDH sobrenadante de culturas. Por fim investigamos a interação DENV-2 com componentes purificados do SC por eletroforese das proteínas. Como achados principais, observamos diminuição na frequência de monócitos CD14+ expressando CR3, CR4 e CD59 em pacientes comparado aos controles saudáveis. De forma interessante, o bloqueio do CR3 levou à diminuição em cerca de 30% da infecção in vitro pelo DENV-2 em monócitos, sem alterar o fenótipo de ativação ou a ativação da caspase-1 destas células. No entanto, com o bloqueio de CR3, detectamos diminuição na produção de TNF-alfa e IFN-alfa. Não observamos diferença significativa na frequência de linfócitos T expressando CR3, CD46, CD55 e CD59 de em pacientes-Dengue-4 Apesar disso, os linfócitos T CR+ apresentaram um perfil ativado coexpressando CD29, CCR5 e CD107a. Na infecção pelo DENV, independente do sorotipo viral, detectamos elevados níveis circulantes de SC5b-9. Na infecção pelo DENV-1/-2 os níveis mais elevados de SC5b-9 foram observados em pacientes que apresentaram sangramentos e extravasamento plasmático, enquanto nos pacientes-DENV-4, os níveis de SC5b-9 foram correlacionados diretamente com a manutenção da integridade vascular. Entretanto, o SC5b-9 parece estar associado com a liberação de LDH intracelular. Finalmente, vimos que DENV-2 promove a clivagem de C3 na ausência de fator D. Nossos dados sugerem que uma frequência diminuída de monócitos expressando CR3 em pacientes poderia ser uma tentativa de controle da infecção, uma vez que detectamos diminuição da infecção, com o bloqueio de CR3 in vitro e ainda, vimos que o DENV-2 modula a clivagem de C3. O aumento da frequência de linfócitos T CR+ coexpressando moléculas de ativação sugere que células de perfil ativado, capazes de controlar a infecção, estariam protegidas da lise via SC. Confirmamos que o SC está ativado na Dengue, pelos altos níveis de SC5b-9. Entretanto, os níveis de SC5b-9 foram associados com a gravidade nos pacientes-DENV-1/-2 enquanto, nos pacientes-DENV-4 foram correlacionados com a manutenção da integridade vascular. Esses dados sugerem que a ativação do SC estaria relacionada diferencialmente com a patogênese de acordo com o sorotipo da infecçãoThe complement system (CS) develops an important role in the infection control, by direct elimination of pathogens and regulation o f the immune response. However, a deregulated CS activation is responsible to induce deleterious effects to the host, contributing to the pathogenesis of several diseases, including Dengue. Besides this, the involvement of the CS on Dengue virus (DENV) inf ection still has many aspects to be investigated. So, we evaluated the CS contribution during in vitro infection of monocytes by DENV; the profile of complement receptors (CR) CR1, CR2, CR3, CR4, CD46,CD55 and CD59 expression on circulating monocytes and T cells from DENV - 1, - 2 or - 4 infected patients by flow cytometry. We assessed the levels of SC5b - 9 and cytokines in patients by ELISA. We evaluated the contribution of the CS activation on endothelial permeability and viability by using an in vitro model wi th endothelial cells by accessing the transendothelial electric resistence (TEER) and LDH release on culture supernatants. Finally, the interaction of DENV - 2 and purified components was evaluated by electrophoresis of proteins. As principal findings, we ob served decreased frequencies of CD14 monocytes expressing CR3, CR4 and CD59 in patients compared to healthy controls. Interestingly, the blockage of CR3 resulted in around 30% reduction of the DENV - 2 infection in monocytes in vitro , without alteration on activation phenotype neither on caspase - 1 activation, of these cells. However, with CR3 - blocking we detected decreased production of TNF - alpha and IFN - alpha. Unaltered frequencies of T cells expressing CR3, CD46, CD55 and CD59 were found in DENV - 4. Beside s, T cells CR+ presented an activated profile, by the coexpression of CD29, CCR5 e CD107a. In DENV infection, regardless the viral serotype, we detected increased levels of circulating SC5b - 9. However among the two groups of patients we saw controversial r esults. In the DENV - 1/ - 2 infection higher levels of SC5b - 9 were detected in patients with bleeding and vascular leakage, while in DENV - 4 patients, the levels of SC5b - 9 were directly correlated with the maintenance of the vascular integrity. Although, the SC5b - 9 levels is associated to release of intracellular LDH. Finally, we saw that DENV - 2 promotes C3 cleavage in the absence of factor D. Our data suggest that a decreased frequency of CR3 - expressing monocytes in DENV - patients would aim to control the infe ction, since in vitro , we detected decreased infection when CR3 was blocked e also, DENV - 2 seems to modulate the cleavage of C3. The increased frequency of T cells CR+ coexpressing activation molecules suggest that cells with activated profile, able to con trol the infection, would be protected of CS mediated lysis. We confirmed that CS is activated during Dengue, by the high levels of SC5b - 9. However, the levels of SC5b - 9 were associated with to severity in DENV - 1/ - 2 patients, while in DENV - 4 it was directl y correlated with the maintenance of the vascular integrity. These data suggest that CS activation would be differentially related to the disease pathogenesis in accordance to the serotype of the infectio

Cissampelos sympodialis has anti-viral effect inhibiting dengue non-structural viral protein-1 and pro-inflammatory mediators

Dengue is the most important viral infection transmitted among humans by arthropod-borne. There are currently no vaccines or specific therapeutical treatment. Therefore, immunomodulatory compounds from plants have been widely examined for their antiviral effects. Cissampelos sympodialis Eichler, Menispermaceae, has scientifically proven to present immunomodulatory activities. Here we assessed the antiviral activity of leaf hydroalcoholic extract, warifteine or methylwarifteine from C. sympodialis in an in vitro dengue virus infection model. The results demonstrated that leaf hydroalcoholic extract or warifteine/methylwarifteine treatment did not reduce dengue virus-Ag+ hepatocyte (Huh-7 cell) rates in present experimental conditions. However, we assessed the potential antiviral effect of leaf hydroalcoholic extract or warifteine/methylwarifteine on dengue virus-infection by the production of inflammatory molecules, TNF-α, MIF, IL-8 and PGE2. Dengue virus infection enhanced TNF-α, MIF, IL-8 and PGE2 production in infected Huh-7 cells and leaf hydroalcoholic extract but not warifteine/methylwarifteine treatments, significantly reduced these molecules in infected cells. In dengue virus-infected Huh-7 cells, non-structural protein-1 is produced and leaf hydroalcoholic extract significantly inhibited it independently of alkaloids. Our findings imply that leaf hydroalcoholic extract may attenuate dengue virus infection in Huh-7 cells by inhibiting the enhanced of pro-inflammatory mediators and non-structural protein-1 production induce by dengue virus independently of warifteine/methywarifteine its major compound. Keywords: Cissampelos sympodialis, Dengue, Huh-7 cells, Cytokines, NS1, Wariftein

Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7

Submitted by Sandra Infurna ([email protected]) on 2018-02-11T12:37:33Z No. of bitstreams: 1 cintiasilva_melo_etal_IOC_2017.pdf: 2999377 bytes, checksum: 694c8a639e979dd7ee437fe2a8c9cf99 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-02-11T12:46:26Z (GMT) No. of bitstreams: 1 cintiasilva_melo_etal_IOC_2017.pdf: 2999377 bytes, checksum: 694c8a639e979dd7ee437fe2a8c9cf99 (MD5)Made available in DSpace on 2018-02-11T12:46:26Z (GMT). No. of bitstreams: 1 cintiasilva_melo_etal_IOC_2017.pdf: 2999377 bytes, checksum: 694c8a639e979dd7ee437fe2a8c9cf99 (MD5) Previous issue date: 2017Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Amazonas. Escola Normal Superior. Manaus, Am, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Embrapa Agroindústria Tropical. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features

Effect of CR blocking antibody on the maturation of monocytes infected <i>in vitro</i> with DENV-2.

<p>Flow cytometry was performed to determine the frequency of anti-DENV complex staining on monocytes. In parallel, DENV NS1 antigen was made in the supernatant of these cultures of monocytes by ELISA. Both methods were used when monocytes were pre-treated or not with 10 µg/ml of blocking mAbs against CR1 (CD35), CR3 (CD11b), CR4 (CD11c), CD18 or IgG, alone or combined, followed by DENV-2 infection. (A) Specific gating strategies were used to select the monocyte population as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102014#pone-0102014-g001" target="_blank">Figure 1</a>. The percentage of anti-DENV complex monocytes was determined using quadrant statistics applied on forward scatter (FSC) versus FL-8/anti-DENV complex/Alexa-Fluor647 dot-plot distributions. Representative dot plots are shown for non-infected and DENV-2-infected monocytes with or without CR3 (CD11b) blocking. (B) Box represents independent experiments in nine different subjects. Vertical bars indicate the median and interquartile range for each condition. (C) The mean percentage of DENV infection inhibition was determined by the reduction of the viral protein NS1 amounts on supernatants by the following formula: O.D. viral protein NS1 amounts condition test ×100/O.D. viral protein NS1 in DENV-2-infected monocytes reduced of the 100, utilising samples collected from 8 different subjects. OD, optical density. The Mann-Whitney U-test was used in order to analyse differences among different conditions. Statistically significant p-values for differences between patients and controls are shown above the pairs.</p

Effect of complement receptor blocking on the presentation of major histocompatibility complex class II molecules and the expression of co-stimulatory molecules on monocytes infected with the dengue virus.

<p>Effect of complement receptor blocking on the presentation of major histocompatibility complex class II molecules and the expression of co-stimulatory molecules on monocytes infected with the dengue virus.</p

Effect of CR blocking antibody on the cytokine production and activation of caspase-1 of monocytes infected <i>in vitro</i> with DENV-2.

<p>(A) The specific scatter gate using the combination of side scatter and forward scatter, followed by the percentage using quadrant statistics applied on FL5/PECy5.5 (CD14) versus FL2/PE (CD11c) dot-plot distributions for caspase-1 analysis. Representative FAM FLICA Caspase 1 marker was quantified using the percentage determined by histogram distribution plots from analysis of the monocytes that were non-infected (NI), infected and infected but pre-treated with CR3 (CD11b/CD18) blocking antibody. Supernatant concentrations of TNF-α (B) and IFN-α (C) were quantified by CBA and ELISA, respectively, after DENV-2 infection of monocytes. The mean percentage reduction of both cytokines when cultures were pre-treated with CR blocking antibody is shown (C). The Wilcoxon-test was used in order to analyse the differences among the various conditions. Statistically significant p-values for differences between patients and controls are shown above the pairs.</p

Expression of complement receptors on monocytes from DENV-infected patients and healthy controls.

<p>Flow cytometry procedures were performed to determine complement receptor (CR) expression on monocytes from patients compared with those obtained from non-infected (NI) individuals (controls). (A) The analysis of monocytes was performed by establishing a specific scatter gate using the combination of anti-cell surface antigens and laser forward scatter (FSC) to discriminate, and the monocytes were gated as FSC High (300–500) CD14^High+. (B) The expression of CR markers was performed using the percentage determined by histogram distribution applied on events <i>versus</i> FL1/FITC (CR1 [CD35) and CD59), FL2/PE (CR4 [CD11c]), FL5/PECy7 (CR3 [CD11b]) or FL8/APC (CR2 [CD21]). Representative histograms of CR+ on monocytes from a healthy control (black line), DF patient (blue line), severe DF patient (red line) and isotype control (gray line). (C–G) Graphic representations of the frequency of CR1 (CD35), CR2 (CD21), CR3 (CD11b), CR4 (CD11c) and CD59 among CD14+ monocytes population from controls (NI = O) and patients with distinct clinical forms of dengue disease (DF; DF/WS; Severe DF). Each point represents an individual analysed. The horizontal line represents the medians, and the vertical bars represent the interquartile ranges for the various populations. The Mann-Whitney U-test was used in order to analyse differences between control and patient groups. Statistically significant p-values for differences between patients and controls are shown above the pairs. SSC, side scatter.</p