Effects of Oral Administration of Moringa oleifera Lam on Glucose Tolerance in Goto-Kakizaki and Wistar Rats

Medicinal plants constitute an important source of potential therapeutic agents for diabetes. In the present study, we investigated the effects of Moringa oleifera (MO) Lam, Moringacea, on glucose tolerance in Wistar rats and Goto-Kakizaki (GK) rats, modeled type 2 diabetes. Major polyphenols in MO powder were quercetin glucosides, rutin, kaempferol glycosides and chlorogenic acids by HPLC analysis. As the results of glucose tolerance test, MO significantly decreased the blood glucose at 20, 30, 45and 60 min for GK rats and at 10, 30 and 45 min for Wistar rats (p<0.05) compared to the both controls after glucose administration. The area under the curve of changes in the blood glucose was significantly higher in the GK control group than in the GK plus MO group (p<0.05) in the periods 30–60 min and 60–120 min. Furthermore, MO significantly decreased stomach emptying in GK rats (p<0.05). The results indicated that MO has an ameliorating effect for glucose intolerance, and the effect might be mediated by quercetin-3-glucoside and fiber contents in MO leaf powder. The action of MO was greater in GK rats than in Wistar rats

High-Phosphorus Diet Induces Osteopontin Expression of Renal Tubules in Rats

High-phosphorus (P) diet induces nephrocalcinosis in rats; however, the mechanism for onset of this disorder is unclear. The calcium (Ca) deposits in kidney are a form of hydroxyapatite, while osteopontin is combined with hydroxyapatite. Based on these observations, we speculated that the osteopontin play an important role in the formation of the Ca deposits induced by high-P diet. This study was investigated the effect of high-P diet on osteopontin expression in kidney. Female Wistar rats were fed diets containing P concentrations of either 0.3% (control diet) or 1.5% (high-P diet) for 14 days. On von Kossa staining, Ca deposits were seen in the tubules of the cortex, outer medulla and inner medulla in rats fed on the high-P diet. Expression of osteopontin was confirmed in rats fed on the high-P diet by immunohistochemical staining, and the localization of this protein was in the same region as the Ca deposits. On the other hand, no evidence of Ca deposits and osteopontin expression was observed in the tubules of the cortex, outer medulla or inner medulla of rats fed on the control diet. These results suggest that high-P diet induces osteopontin expression in the renal tubules. Moreover, our results suggest that increase in osteopontin expression in the renal tubules is presumably involved in the formation of Ca deposits induced by high-P diet

Probable association of T Tauri stars with the L1014 dense core

Using the Wide Field Grism Spectrograph 2 (WFGS2), we have carried out slit-less spectroscopy, g'r'i' photometry, and slit spectroscopy on the L1014 dense core. We detected three Halpha emission line stars. We interpret one as weak-line T Tauri star (WTTS) and the others as classical T Tauri stars (CTTS). Since their g'-i' colors and/or classified spectral types are consistent with those of T Tauri stars and two of them show less extinction than the cloud, these three stars are likely to be T Tauri stars associated with L1014. Adopting an age range for T Tauri stars, 1-10 Myr, the color-magnitude diagram suggests a distance of ~400-900 pc, rather than the previously assumed distance, 200 pc. This could strongly affect on the mass estimate of L1014-IRS, which is thought to be either a very young protostar or proto-brown dwarf.Comment: 5 pages, 5 figures, to be published in Vol.58, No.5, October 25, 200

Distribution and Major Sources of Flavonoid Intakes in the Middle-Aged Japanese Women

We estimated the intake of individual flavonoids in a cross sectional study and clarified the major sources contributing to the flavonoid levels in the middle-aged Japanese women by a 24-h weighed dietary record study. The subjects included in the study were 516 free-living women. Each subject completed a 24-h weighed dietary record and received a health check-up. We used the Functional Food Factor database for estimating the intake of 5 major flavonoid intakes, i.e. flavan-3-ols, isoflavones, flavonols, flavanones and flavones. The mean intake of flavan-3-ols, isoflavones, flavonols, flavanones and flavones was 1277, 216, 58, 31 and 15 µmol/d, respectively. The richest source of flavan-3-ols was green tea. The 3 major food sources of isoflavone were the processed soy foods and those of flavonol were the onion, moroheiya (nalta jute) and Japanese radish leaves. Grapefruit and citrus fruit juices were the major sources of flavanones, and tsurumurasaki (malabar spinach), green pepper and grapefruit were the main sources of flavone. Furthermore, analysis of sub-samples from middle-aged Japanese women indicated that there may be an association between flavonoid intake and the levels of oxidized LDL, which might be related to the incidence of cardiovascular diseases

Effects of excitation light intensity on parathyroid autofluorescence

The intraoperative identification and preservation of the parathyroid glands are vital techniques, which are largely dependent on a surgeon’s experience. Therefore, a simple and reproducible technique to identify the parathyroid glands during surgery is needed. Parathyroid tissue shows near-infrared (NIR) autofluorescence, which enables the intraoperative identification of the parathyroid gland. We herein present two cases that underwent surgery on the parathyroid glands, which were observed using the NIR fluorescence imaging system LIGHTVISION® (Shimazu, Kyoto, Japan). In a case of papillary thyroid carcinoma, the system was adopted to preserve normal parathyroid glands during left hemithyroidectomy. The left lower parathyroid gland was identified using the imaging system under white light; however, its autofluorescence was visualized more clearly with the excitation light of NIR. In a case of primary hyperparathyroidism due to MEN1, the system was adopted to identify and remove all of the parathyroid glands during total parathyroidectomy. The autofluorescence of diseased glands was weaker than that of normal glands, even with the excitation light of NIR. When the parathyroid glands were irradiated with a red laser pointer, the intensity of autofluorescence significantly increased. However, the largest gland, which was pathologically proven to contain strongly proliferating chief cells, did not show autofluorescence. These results suggest that normal or less diseased parathyroid glands, which are generally small and difficult to identify during surgery, showed relatively strong autofluorescence. A stronger excitation light increases the autofluorescence of parathyroid glands, which enhances sensitivity for detecting parathyroid glands during surgery. In conclusion, LIGHTVISION® is a useful device to identify parathyroid glands and an additional excitation light of a red laser pointer increases the detection sensitivity

Effects of High Phosphorus Diet on Bone Metabolism-Related Gene Expression in Young and Aged Mice

In this study, the effects of high phosphorus (P) diet on bone metabolism-related gene expression were investigated in young and aged mice. Twelve- and 80-week-old ddY male mice were divided into two groups, respectively, and fed a control diet containing 0.3% P or a high P diet containing 1.2% P. After 4 weeks of treatment, serum parathyroid hormone (PTH) concentration was significantly higher in the high P groups than in the control groups in both young and aged mice and was significantly higher in aged mice than in young mice fed the high P diet. High P diet significantly increased receptor activator of NF-κB ligand (RANKL) mRNA in the femur of both young and aged mice and significantly increased the RANKL/osteoprotegerin (OPG) mRNA ratio only in aged mice. High P diet significantly increased mRNA expression of transient receptor potential vanilloid type 6, calbindin-D9k, and plasma membrane Ca2+-ATPase 1b in the duodenum of both young and aged mice. These results suggest that high P diet increased RANKL mRNA expression in the femur and calcium absorption-related gene expression in the duodenum regardless of age. Furthermore, the high P diet-induced increase in PTH secretion might increase the RANKL/OPG mRNA ratio in aged mice

MONITORING ANAPLASTIC THYROID CANCER MODELS BY PET/CT

Anaplastic thyroid carcinoma (ATC) is a rare type of thyroid carcinoma with a poor prognosis. Thus, suitable preclinical tumor models are required for the development of new ATC therapies. In the present study, orthotopic tumor xenograft models were established using ATC cell lines and SCID mice, and tumor invasion and the effects of anticancer drugs were evaluated using positron emission tomography/computed tomography (PET/CT) to repeatedly and non-invasively monitor these models. Three ATC cell lines (8305c, 8505c, and ACT-1) were used. Their sensitivities to two anticancer drugs (paclitaxel and lenvatinib) were investigated. The 8505c cell line was orthotopically implanted into SCID mice, which were then divided into three groups: no chemotherapy, paclitaxel (5 mg/kg, administered intraperitoneally, every week), and lenvatinib (5 mg/kg, oral route, every day) groups. PET/CT was performed and tumor growth and the effects of anticancer drugs based on tumor volume and fludeoxyglucose (FDG) uptake were evaluated. 8505c cells exhibited the highest sensitivity to the anticancer drugs. In mice implanted with 8505c cells, continuous increases in FDG uptake associated with tumor growth were detected on PET/CT in the group that received no chemotherapy. The tumor volume and FDG uptake increased by 91.5- and 2.4-fold, respectively, within 2 weeks. The increase observed in tumor volume was 26.9- and 12.2-fold in the paclitaxel and lenvatinib groups, respectively, within 2 weeks. Furthermore, the increase in FDG uptake was 1.8-fold and 1.6-fold in the paclitaxel and lenvatinib groups, respectively, within 2 weeks. In our orthotopic SCID mouse model, tumor growth and the effects of anticancer drugs were repeatedly and non-invasively monitored using PET/CT. The present method is useful for the development of new ATC treatments

ターミナルケアの授業における学生の死生観に関する検討

本学看護学科3年次生に開講されているターミナルケアの授業を通して学生の死生観の変化を明らかにし今後の教育の方向性を模索することを目的に授業前後で質問紙による調査をした。対象は,本学看護学科3年次生,50名であり,期間は平成18年4月,7月の2回である。その結果,以下のことが明らかになった。1)死別体験,葬儀参列の体験が8～9割があり体験率は高いと言えた。2)死に対する態度の尺度,死生観尺度,死のイメージ尺度(SD法)の授業前後の比較では授業後が有意に高かった。このことから,授業は学生の死生観が肯定的に変化することに大きな意義を持っていたと考えられた。3)死別体験・葬儀参列の体験・看取り体験と各尺度との関連は一部を除いて有意差はなかった。このことから看護学生の死生観を形成する上でこれらの体験は大きな影響を及ぼしていないと考えられた。4)授業は,専門の講師からの講義や学生が自ら死について考えられるような教材であったが,授業後においても死を否定的に考える学生もいるので今後の授業内容の検討が必要であると考えられた。今回の調査より,ターミナルケアの授業は,学生の肯定的な死生観育成に大きな影響を及ぼしていた。しかし,授業後においても死を否定的に捉える学生はいるので,授業のあり方の継続的な検討が示唆された。This study was conducted to determine changes in students\u27 view of death after they have completed the course on terminal care for third year nursing students, so as to help us decide on how the course should be taught in the future. Fifty students were surveyed before and after the course in April and July of 2006. The study revealed the followings. 1) As high as between 80 and 90% of the students have had death in their family or attended a funeral. 2) The semantic differential scales on attitude toward death, view of death, and image of death were significantly higher after students completed the course, suggesting the lectures played an important role in the positive change on students\u27 view of life-and-death. 3) The relevance of the experience of bereavement, funeral attendance, or seeing someone die on these scales was largely insignificant, indicating these experiences do not have any great influence on how a nursing student developed his or her view on death. 4) The course consisted of lectures by instructors specialized in this field and discussions among students about their own thoughts on death. The fact that some students still thought about death negatively after completing the course suggests that the contents of the course work should be reevaluated. The surveys revealed that the course on terminal care had a great impact on helping the students to develop a positive view on death. Nevertheless, the fact that some students continued to think of death negatively suggested there is still a need to reevaluate how the course has been taught

Bioavailability of prenyl quercetin

Prenyl flavonoids are widely distributed in plant foods and have attracted appreciable attention in relation to their potential benefits for human health. Prenylation may enhance the biological functions of flavonoids by introducing hydrophobic properties in their basic structures. Previously, we found that 8-prenyl naringenin exerted a greater preventive effect on muscle atrophy than nonprenylated naringenin in a mouse model. Here, we aimed to estimate the effect of prenylation on the bioavailability of dietary quercetin (Q). The cellular uptake of 8-prenyl quercetin (PQ) and Q in Caco-2 cells and C2C12 myotube cells was examined. Prenylation significantly enhanced the cellular uptake by increasing the lipophilicity in both cell types. In Caco-2 cells, efflux of PQ to the basolateral side was <15% of that of Q, suggesting that prenylation attenuates transport from the intestine to the circulation. After intragastric administration of PQ or Q to mice or rats, the area under the concentration-time curve for PQ in plasma and lymph was 52.5% and 37.5% lower than that of Q, respectively. PQ and its O-methylated form (MePQ) accumulated at much higher amounts than Q and O-methylated Q in the liver (Q: 3400%; MePQ: 7570%) and kidney (Q: 385%; MePQ: 736%) of mice after 18 d of feeding. These data suggest that prenylation enhances the accumulation of Q in tissues during long-term feeding, even though prenylation per se lowers its intestinal absorption from the diet