Fast walking is a preventive factor against new-onset diabetes mellitus in a large cohort from a Japanese general population

博士（医学）福島県立医科大

Stimulating Effect of High Concentration of Calcium Ion on the Polymerization of the Tubulin-Colchicine Complex. −Relationship between Magnesium and Calcium−

The polymerization reaction was reversible by the addition of calcium and/or EGTA and cooling. The extent of inhibition was affected by the pH and magnesium ion concentration. Maximum inhibition of polymerization was observed at a concentration of around 100µM calcium ion. The presence of calcium in a polymerization buffer caused the critical concentration to increase. At above 250µM calcium ion, no inhibition was observed. The inhibitory effect is due to the high-affinity sites of calcium to the tubulin-colchicine complex and the stimulating effect is due to low-affinity sites. Abbreviations: EGTA, Ethlene-bis (oxyehylenenitrilo) tetraacetic acid; GTP, guanosine 5'-triphosphate