13 research outputs found
Chemical analysis of a genome wide polyketide synthase gene deletion library in Aspergillus nidulans
Induction of metabolites by SmrA.
<p>A) UHPLC-QTOFMS extracted ion chromatogram of m/z 251 (MF, [M+H]<sup>+</sup>), 289 (JH-III, [M+Na]<sup>+</sup>), 307 (JH-diol, [M+H]<sup>+</sup>) and 335 (X2, [M+H]<sup>+</sup>) recorded in positive mode of extracts from the strain constitutively expressing <i>smrA</i> (top) and reference (middle) grown under high salt conditions. Chromatograms are normalized by intensity. Chemical structures of JH-diol, compound <b>2</b>, JH-III and MF are embedded above the corresponding signal peaks. Bottom panel depicts extracted ion chromatogram of m/z 289 (JH-III, [M+Na]<sup>+</sup>) of an authentic JH-III standard (65% pure) purchased from Sigma Aldrich. Note that the standard contains several impurities. Panel B): Corresponding mass spectra of JH-diol, compound <b>2</b>, JH-III and MF in the mutant strain constitutively expressing <i>smrA</i> as well as the authentic JH-III standard. Chemical structure of the corresponding molecule is embedded in each panel.</p
Insect grazing induced alterations of secondary metabolites in <i>A. nidulans</i>.
<p>Quantification of secondary metabolites: JH-III, JH-diol, austinol, dehydroaustinol, nidulanin A and sterigmatocystin from LC-HRMS analysis. For each metabolite, columns display the average and error bars the standard deviation. Statistical analysis was performed with pair-wise comparisons using the student's t-test. Panel A) comparison of austinol, dehydroaustinol, nidulanin A and sterigmatocystin levels in NID477 and NID545. Panel B) comparison of JH-III and JH-diol levels in NID545 and NID477. Panel C) <i>D. melanogaster</i> feeding significantly increases accumulation of JH-III (p-values; 0,0288 and 0,00723) and JH- diol (p-values; 0,0006 and 0,02415) in NID477 and NID545, respectively.</p