Insulin induces a positive relationship between the rates of ATP and glycogen changes in isolated rat liver in presence of glucose; a (31)P and (13)C NMR study

BACKGROUND: There is an emerging theory suggesting that insulin, which is known to be the predominant postprandial anabolic hormone, is also a major regulator of mitochondrial oxidative phosphorylation in human skeletal muscle. However, little is known about its effects in the liver. Since there is a theoretical relationship between glycogen metabolism and energy status, a simultaneous and continuous investigation of hepatic ATP and glycogen content was performed in intact and isolated perfused liver by (31)P and (13)C nuclear magnetic resonance (NMR) The hepatic rates of ATP and glycogen changes were evaluated with different concentrations of insulin and glucose during continuous and short-term supply. RESULTS: Liver from rats fed ad libitum were perfused with Krebs-Henseleit Buffer (KHB)(controls) or KHB containing 6 mM glucose, 30 mM glucose, insulin alone, insulin + 6 mM glucose, insulin + 30 mM glucose. In the control, glycogenolysis occurred at a rate of -0.53 ± 0.021 %·min(-1) and ATP content decreased at a rate of -0.28 ± 0.029 %·min(-1). In the absence of insulin, there was a close proportional relationship between the glycogen flux and the glucose concentration, whereas ATP rates never varied. With insulin + glucose, both glycogen and ATP rates were strongly related to the glucose concentration; the magnitude of net glycogen flux was linearly correlated to the magnitude of net ATP flux: flux(glycogen )= 72.543(flux(ATP)) + 172.08, R(2 )= 0.98. CONCLUSION: Only the co-infusion of 30 mM glucose and insulin led to (i) a net glycogen synthesis, (ii) the maintenance of the hepatic ATP content, and a strong positive correlation between their net fluxes. This has never previously been reported. The specific effect of insulin on ATP change is likely related to a rapid stimulation of the hepatic mitochondrial oxidative phosphorylation. We propose that variations in the correlation between rates of ATP and glycogen changes could be a probe for insulin resistance due to the action of substrates, drugs or pathologic situations. Consequently, any work evaluating insulin resistance on isolated organs or in vivo should determine both ATP and glycogen fluxes

Quelle place pour le complément alimentaire dans l'arthrose à l'officine ?

Les compléments alimentaires représentent un marché en pleine évolution à l officine. Leur statut règlementaire en tant qu aliments à l aspect de médicaments semble les situer à la frontière du domaine thérapeutique et attire un grand nombre de personnes. Ils servent à compléter le régime alimentaire et ont pour objectif d aider notre organisme à garder la santé, voire à l améliorer, au moyen de différents ingrédients actifs : vitamines, minéraux, plantes et autres substances d origines animale ou biotechnologique (propolis, glucosamine, probiotiques ). Depuis 2002, les compléments alimentaires bénéficient d une règlementation spécifique complexe au niveau européen. La reconnaissance de leur bénéfice physiologique passe par l obtention d allégations de santé, autorisées seulement si la preuve scientifique du bénéfice est apportée. L arthrose touche 9 à 10 millions de Français. Cette maladie caractérisée par des douleurs articulaires, concerne essentiellement la population âgée. Des traitements conventionnels existent, tels que les Anti-Inflammatoires Non Stéroïdiens (AINS), les antalgiques et les Anti-Arthrosiques Symptomatiques d Action Lente (AASAL : glucosamine, chondroïtine ), mais sont parfois mal tolérés ou insuffisants. Considérés comme des adjuvants thérapeutiques par certains praticiens, les compléments alimentaires sont de plus en plus conseillés au cours de l arthrose. L objectif de cette thèse est d étudier les principaux ingrédients utilisés dans les compléments alimentaires à visée articulaire, inscrits au Vidal 2012, afin d évaluer leur possible intérêt en complément ou en remplacement des traitements thérapeutiques classiques de l arthrose.Food supplements are in progress in the pharmaceutical market. Their special food status, close to the therapeutic medicine attracts a large number of people. The food supplement is used to supplement the diet. It aims to help our body to stay healthy or to improve it. It may consist of different active ingredients: vitamins, minerals, herbs and other animal or biotechnological substances (propolis, glucosamine, probiotics ...). Since 2002, food supplements have a specific European legislation, complex and constantly evolving. Recognition of their physiological benefit with health claims is permitted only if the scientific evidence for benefit is demonstrated. Osteoarthritis affects 9 to 10 million French. This disease is characterized by joint pain and concerns mainly the elderly population. Conventional treatments exist, such as Steroidal Anti-Inflammatory drugs (NSAIDs), analgesics and symptomatic slow acting drugs (chondroitin sulphate, glucosamine sulphate ), but are sometimes poorly tolerated or insufficient. Considered as "therapeutic adjuvants" by some health practitioners, food supplements are increasingly recommended in osteoarthritis. The objective of this thesis is to study the main ingredients used in food supplements for joints, registered in Vidal 2012 (French register of medicins) to assess their possible interest in addition or replacing of conventional arthrosis therapeutical treatments.ANGERS-BU Médecine-Pharmacie (490072105) / SudocSudocFranceF

Site effects observations and mapping on the weathered volcanic formations of Mayotte Island

Since the 2000s, local seismic hazard studies have shown that Mayotte Island presented superficial geological formations prone to lithological site effects. The seismic sequence initiated in May 2018 confirmed the importance of such effects, both in terms of intensity and spatial extension. The analysis of the recorded strong motions showed that weathered volcanic formations are prone to significant site effects with mean amplification factors for peak ground acceleration (PGA) between 1.4 and 4.9 and that a complex combination of lithological and topographic site effects is in action. We thus implement a regional scale map of site effects for the fast calculation of strong motion and damage maps for crisis management purposes. We also provide a first estimate of key site parameters for eight stations: surface geology, resonance frequency, an amplification factor proxy for PGA, a ${V}_{{S},30}$ value, if available, and an estimated EC8 soil class

Older adults exhibit a more pronounced modulation of beta oscillations when performing sustained and dynamic handgrips

Muscle contractions are associated with a decrease in beta oscillatory activity, known as movement-related beta desynchronization (MRBD). Older adults exhibit a MRBD of greater amplitude compared to their younger counterparts, even though their beta power remains higher both at rest and during muscle contractions. Further, a modulation in MRBD has been observed during sustained and dynamic pinch contractions, whereby beta activity during periods of steady contraction following a dynamic contraction is elevated. However, how the modulation of MRBD is affected by aging has remained an open question. In the present work, we investigated the effect of aging on the modulation of beta oscillations and their putative link with motor performance. We collected magnetoencephalography (MEG) data from younger and older adults during a resting-state period and motor handgrip paradigms, which included sustained and dynamic contractions, to quantify spontaneous and motor-related beta oscillatory activity. Beta power at rest was found to be significantly increased in the motor cortex of older adults. During dynamic hand contractions, MRBD was more pronounced in older participants in frontal, premotor and motor brain regions. These brain areas also exhibited age-related decreases in cortical thickness; however, the magnitude of MRBD and cortical thickness were not found to be associated after controlling for age. During sustained hand contractions, MRBD exhibited a decrease in magnitude compared to dynamic contraction periods in both groups and did not show age-related differences. This suggests that the amplitude change in MRBD between dynamic and sustained contractions is larger in older compared to younger adults. We further probed for a relationship between beta oscillations and motor behaviour and found that greater MRBD in primary motor cortices was related to degraded motor performance beyond age, but our results suggested that age-related differences in beta oscillations were not predictive of motor performance

Neurophysiological changes induced by music-supported therapy for recovering upper extremity function after stroke: a case series

Music-supported therapy (MST) follows the best practice principles of stroke rehabilitation and has been proven to instigate meaningful enhancements in motor recovery post-stroke. The existing literature has established that the efficacy and specificity of MST relies on the reinforcement of auditory-motor functional connectivity in related brain networks. However, to date, no study has attempted to evaluate the underlying cortical network nodes that are key to the efficacy of MST post-stroke. In this case series, we evaluated changes in connectivity within the auditory-motor network and changes in upper extremity function following a 3-week intensive piano training in two stroke survivors presenting different levels of motor impairment. Connectivity was assessed pre- and post-training in the α- and the β-bands within the auditory-motor network using magnetoencephalography while participants were passively listening to a standardized melody. Changes in manual dexterity, grip strength, movement coordination, and use of the upper extremity were also documented in both stroke survivors. After training, an increase in the clinical measures was accompanied by enhancements in connectivity between the auditory and motor network nodes for both the α- and the β-bands, especially in the affected hemisphere. These neurophysiological changes associated with the positive effects of post-stroke MST on motor outcomes delineate a path for a larger scale clinical trial

Evaluation globale standardisée systématique des rhumatismes inflammatoires chroniques: intérêts et limites

Introduction: National and international recommendations call for an annual standardized systematic holistic review in the management of chronic inflammatory rheumatism (CIR). This includes an assessment of disease activity and severity, as well as patient education on the disease, knowledge of pharmacological and non-pharmacological treatments, adherence to treatment and screening for comorbidities. Our study aims to recall the definition of a holistic review (HR), to present the evidence of their effectiveness and to give an overview of HR practices in France. Methods: A literature review was conducted in the Pubmed database to identify randomized controlled trials (RCTs) or meta-analyses reporting the efficacy of a multidisciplinary intervention in ICR or other chronic diseases. Two online surveys were sent to all rheumatology departments in France and to a sample of independent rheumatologists, with 34 and 19 questions respectively. These questionnaires were used to determine the profile of the responding center/rheumatologist, the existence of an HR and the obstacles or facilitators to its implementation. Results: Literature search yielded 872 articles, 24 of which were finally included: 16 RCTs and 8 meta-analyses. Only 3 articles concerned ICRs, including one meta-analysis of 10 RCTs in rheumatoid arthritis (RA). Of these 3 studies, 2 RCTs in systemic lupus and systemic sclerosis showed a favorable impact of a multidisciplinary approach on SLEDAI and grip strength and mouth opening respectively, while the meta-analysis in RA showed no benefit on disability or disease activity.The questionnaire was answered by 72 centers and 186 rheumatologists. A third of the centers had already implemented a HR during an day hospitalization. 70 % of centers estimated that they managed more than 10 patients per month, devoting an average of 35 minutes of rheumatologist time and 90 minutes of cumulative time for all other healthcare professionals (HCPs) involved in the program. Most of the HCPs involved were nurses (92 %), dieticians (56 %) and physiotherapists (56 %). The main obstacles to setting up a HR were the lack of paramedical resources, lack of economic value and lack of support from treating rheumatologists, while patient motivation was seen as a facilitating factor. Conclusion: Although HR is recommended, there is little evidence of its effectiveness in ICR. Only 36 % of responding centers have implemented such a program. This survey helps to identify the obstacles and facilitators, and to find solutions for extending this practice

Le gisement paléolithique multistratifié « les Bossats » à Ormesson (Seine-et-Marne, France) : palethnographie ou pâle ethnographie ? Une synthèse des huit premières années de fouille (2009-2016)

editorial reviewedÀ l'évidence, ces vingt dernières années ont vu en France, notamment, se développer en parallèle deux nouvelles façons de traiter le Paléolithique supérieur ancien qui ne sont pas antagonistes d'ailleurs. L'une consiste en une reprise des stratigraphies anciennes dans le Centre et le Sud-Ouest de la France plus spécifiquement et est associée à une meilleure redéfinition des entités culturelles par l'analyse détaillée des différentes composantes des systèmes techniques. L'autre s'efforce d'appliquer à cette période la démarche palethnographique, inféodée historiquement au Magdalénien du Bassin parisien. Il est vrai que peu de gisements autorisaient ce type d'approche, en raison d'une surface fouillée insuffisante ou d'un état de conservation médiocre, mais même lorsque les découvertes s'y prêtaient, le manque de temps et d'investissement freinait également toute velléité d'une étude approfondie des sites en question, qui aurait alors débouché sur une lecture palethnographique des lieux et des artefacts. À l'issue d'un PCR mené entre 1999 et 2005, nous pouvions ainsi légitimement nous demander si nous étions capables de jouer les ethnologues du passé pour le Paléolithique supérieur ancien dans le Bassin parisien. Les sites identifiés dans le cadre de ce programme de recherche étaient certes nombreux mais représentés surtout par des découvertes de surface, ils ne garantissaient pas un niveau d'analyse digne de ce qui a pu se faire depuis plus de 50 ans à Pincevent ou à Étiolles par exemple (Bodu et al., 2013). Il aura fallu attendre la découverte fortuite du gisement de plein-air d'Ormesson « les Bossats » (Seine-et-Marne, près de Nemours) au début des années 2000 pour que cette question trouve une réponse positive. Concernant, au départ, presqu'exclusivement des vestiges lithiques et osseux attribués au Gravettien, les premières fouilles menées en 2009 permirent d'identifier rapidement un second niveau d'occupation, d'attribution moustérienne. Les campagnes suivantes amenèrent à la découverte de cinq autres niveaux d'occupation paléolithiques, inégaux tant pour la surface couverte que pour l'état de conservation : un second niveau moustérien résultant vraisemblablement de palimpsestes, un ensemble châtelperronien, un autre solutréen, un quatrième badegoulien et enfin entre Châtelperronien et Gravettien, un foyer isolé sans vestiges archéologiques associés. Cette stratigraphie paléolithique de plein-air dilatée est le témoignage d'une forte occupation du lieu pendant près de 30 000 ans, ce qui s'explique notamment par la configuration particulière de la vallée à cet endroit. À la diversité chronologique des occupations préhistoriques répond une diversité des comportements économiques et techniques au sein des différentes sphères d'activités mais également des habitudes spatiales différentes. À l'issue des huit premières années de fouille (2009-2016), le site d'Ormesson « les Bossats » permet ainsi de développer une approche détaillée des comportements techniques, économiques, spatiaux de groupes culturels distincts ayant vécu durant 30 000 ans dans un cadre géomorphologique et plus globalement naturel, relativement identique. Dépassant le jeu de mot facile « palethnographie ou pâle ethnographie ? » à Ormesson « les Bossats », nous proposons ici quelques éléments de réponse

Alien Registration- Baillet, Mathilde Marie (Mount Desert, Hancock County)

https://digitalmaine.com/alien_docs/17408/thumbnail.jp

Les principales urgences médicales chez les bovins

L'hypocalcémie puerpérale, la tétanie d'herbage, les mammites toxiniques, les intoxications, les diarrhées néonatales, l'anoxie néonatale et les septicémies néonatales sont des urgences médicales engageant la vie de l'animal. Une bonne connaissance de ces différentes pathologies est donc indispensable au praticien afin d'être le plus rapide et le plus efficace possible. Les deux premières parties de ce travail comportent les monographies de ces grandes pathologies en fonction de la catégorie d'animaux qu'elles touchent (adulte ou jeune). La troisième partie est un ensemble de fiches synthèse permettant au praticien de retrouver rapidement une information nécessaire au diagnostic et au traitement.TOULOUSE-EN Vétérinaire (315552301) / SudocMAISONS-ALFORT-Ecole Vétérin (940462302) / SudocSudocFranceF