Efeito do exercício físico contínuo e intervalado no peso e perfil bioquímico de ratas Wistar prenhes e consequências no peso da prole

Exercise training is known for its benefits to the body and mind. However, little is known about the effects of endurance training intensity on pregnancy. We tested the effects of continuous and intermittent exercises (maternal swimming) on the biochemical profile of pregnant Wistar rats and the effects of these exercises on the fetal body weight. The pregnant females (n=60) were divided in control group (GC), continuous exercise group (GCON) and intermittent exercise group (GIN). GC group rats did not practice exercises. GON group rats practiced continuous swimming for 45 minutes a day (five days a week) carrying a bag with 5% of its body weight. GIN group rats practiced intermittent swimming (15 seconds of swimming and 15 seconds stopped) for 45 minutes a day (five days a week) carrying a bag with 15% of its body weight. These exercises were made from the day one until 20(0) day of pregnancy. At the end of this period, we analyzed the mother's glycemia, cholesterol, HDL-C, LDL-C and triglycerides. We also analyzed fetal body weight. No significant modifications on glycemia and plasma lipids (except for LDL-C) were observed in the tree groups. We observed reduction on the fetal body weight in the pups that had their mothers practicing exercises: fetal body weight in GC > GIN > GON (4,153 +/- 0,649; 3,682 +/- 0,070 and 3,453 +/- 0,052 respectively). These results showed that the continuous and intermittent exercise only performed in pregnancy period decreases fetus body weight.Univ Marilia, Fac Med & Enfermagem, Marilia, SP, BrazilUniv Marilia, Fac Ciencias Saude, Marilia, SP, BrazilFac Tecnol Alimentos Marilia, Marilia, SP, BrazilUNESP, Fundecif Fundacao Desenvolvimento Ciencias Farmac, Araraquara, SP, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilUnifesp, Dept Morfol, Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilUnifesp, Dept Morfol, Sao Paulo, BrazilWeb of Scienc

Evaluation of level of DNA damage in blood leukocytes of non-diabetic and diabetic rat exposed to cigarette smoke

The objective of the present study was to use the comet assay to evaluate the steady-state level of DNA damage in peripheral blood leukocytes from diabetic and non-diabetic female Wistar rats exposed to air or to cigarette smoke. A total of 20 rats were distributed into four experimental groups (n= 5 rats/group): non-diabetic (control) and diabetic exposed to filtered air; non-diabetic and diabetic exposed to cigarette smoke. A pancreatic beta (beta)-cytotoxic agent, streptozotocin (40 mg/kg b.w.) was used to induce experimental diabetes in rats. Rats placed into whole-body exposure chambers were exposed for 30 min to filtered air (control) or to tobacco smoke generated from 10 cigarettes, twice a day, for 2 months. At the end of the 2-month exposure period, each rat was anesthetized and humanely killed to obtain blood samples for genotoxicity analysis using the alkaline comet assay. Blood wleukocytes sampled from diabetic rats presented higher DNA damage values (tail moment =0.57 +/- 0.05; tail length =19.92 +/- 0.41, p 0.05) and diabetic rats (tail moment= 0.41 +/- 0.03, p > 0.05) exposed to cigarette smoke presented non-significant increases in DNA damage levels compared to control group. In conclusion, our data show that the exposure of diabetic rats to cigarette smoke produced no additional genotoxicity in peripheral blood cells of female Wistar rats. (c) 2007 Elsevier B.V. All rights reserved

Oxidative stress status and lipid profiles of diabetic pregnant rats exposed to cigarette smoke

This study aimed to evaluate the oxidative stress status and the concentrations of triglycerides, cholesterol and total proteins of pregnant rats exposed to the association of diabetes and cigarette smoke. Female Wistar rats were randomly distributed in four experimental groups, according to presence or not of diabetes and the exposure or not to cigarette smoke. Diabetes was induced by streptozotocin (40 mg/kg i.v.) and exposure to cigarette smoke was for 30 min, twice a day, for 2 months. At day 21 of pregnancy, blood was collected for total protein, triglyceride, cholesterol and oxidative stress determinations. Data were analysed by ANOVA followed by Student-Newman-Keuls test (P < 0.05). The association of diabetes and exposure to cigarette smoke was related to the indidence of hypertriglyceridaemia, and this result was due to the severe diabetes and not to exposure to smoke. There was no alteration to protein metabolism in pregnant rats. Diabetes and cigarette smoke exposure led to the activation of the antioxidant system in an attempt to detoxify the organism in face of high lipid peroxidation, which can be characterized by the determination of reactive substances to thiobarbituric acid. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Effects of Passiflora edulis (Yellow Passion) on Serum Lipids and Oxidative Stress Status of Wistar Rats

The aim of this study was to evaluate the effects of Passiflora edulis f. flavicarpa Degener (yellow passion) juice on the lipid profile and oxidative stress status of Wistar rats. Adult male Wistar rats were divided in two groups (n = 8 animals per group): the control group, which received water, and the treated group, which was given P. edulis juice (1,000 mg/kg). Both groups received by gavage treatment twice a day for 28 days. The treated group showed an increased high-density lipoprotein-cholesterol level and decreased low-density lipoprotein-cholesterol and free fatty acid levels compared with the control group. Levels of triglycerides and and very low-density lipoprotein-cholesterol, superoxide dismutase activity, and total glutathione concentration were not statistically different between the two groups, but the thiobarbituric acid-reactive substances concentration (indicating lipid peroxidation) decreased in the treated group. These findings suggests that P. edulis juice in the experimental conditions used showed beneficial effects on lipid profile and improved lipid peroxidation in Wistar rats