UTR introns, antisense RNA and differentially spliced transcripts between Plasmodium yoelii subspecies

Additional file 1. Evaluation of RNA quality from the two NSM parasite samples in agarose gel (a), and a flow chart of data processing and analysis (b)

Patterns of microRNA Expression in Non-Human Primate Cells Correlate with Neoplastic Development In Vitro

MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression post-transcriptionally. They play a critical role in developmental and physiological processes and have been implicated in the pathogenesis of several diseases including cancer. To identify miRNA signatures associated with different stages of neoplastic development, we examined the expression profile of 776 primate miRNAs in VERO cells (a neoplastically transformed cell line being used for the manufacture of viral vaccines), progenitor primary African green monkey kidney (pAGMK) cells, and VERO cell derivatives: spontaneously immortalized, non-tumorigenic, low-passage VERO cells (10-87 LP); tumorigenic, high-passage VERO cells (10-87 HP); and a cell line (10-87 T) derived from a 10-87 HP cell tumor xenograft in athymic nude mice. When compared with pAGMK cells, the majority of miRNAs were expressed at lower levels in 10-87 LP, 10-87 HP, and 10-87 T cells. We identified 10 up-regulated miRNAs whose level of expression correlated with VERO cell evolution from a non-tumorigenic phenotype to a tumorigenic phenotype. The overexpression of miR-376a and the polycistronic cluster of miR-376a, miR-376b and miR-376c conferred phenotypic changes to the non-tumorigenic 10-87 LP cells that mimic the tumorigenic 10-87 HP cells. Thirty percent of miRNAs that were components of the identified miRNAs in our spontaneously transformed AGMK cell model are also dysregulated in a variety of human tumors. These results may prove to be relevant to the biology of neoplastic development. In addition, one or more of these miRNAs could be biomarkers for the expression of a tumorigenic phenotype

Reintegro a su medio familiar de los niños, niñas y adolescentes (NNA) en el Distrito de Buenaventura

Disponible en formato fisico T SOC 41 2020Se pretendió conocer si una de las causas de separación familiar que experimentan los niños, niñas y adolescentes tiene que ver con la falta de pautas de crianza impartidas por los padres, problemas de drogadicción, problemas socioeconómicos o dificultades de escolarización; casos de reintegro que fueron intervenidos por el Instituto Colombiano de Bienestar Familiar, del Distrito de BuenaventuraPREGUNTA DE INVESTIGACIÓN Y OBJETIVOS 8 1.1 PREGUNTA DE INVESTIGACION 14 1.2 OBJETIVOS 14 1.2.1 OBJETIVO GENERAL 14 1.2.2 OBJETIVOS ESPECÍFICOS 14 2. MARCO TEÓRICO 16 3. METODOLOGÍA 25 3.1 PROCEDIMIENTO 25 3.2 POBLACIÓN 27 3.3 TÉCNICAS E INSTRUMENTOS DE RECOLECCIÓN 28 3.4 FASES DE LA PASANTÍA 29 4. ANÁLISIS DE LA INFORMACIÓN 31 5 EXPERIENCIA PASANTIAS 45 CONCLUSIÓNES Y RECOMENDACIONES 49 CONCLUSIÓNES 49 RECOMENDACIONES 50 REFERENCIAS 52 ANEXO 11Tesis (Sociología) - Universidad del Pacífico. Facultad de Humanidades y Bellas Artes Programa de Sociología, 2020PregradoSociólogo(a

Correlation between expression of miRNAs and the neoplastic phenotype.

<p>The qRT-PCR values of over-expressed miRNAs (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014416#pone-0014416-t003" target="_blank">Table 3</a>) were used to locate on the X-axis the position of seven lines of immortalized AGMK cells relative to pAGMK cells. Based on the relative magnitude of the expression levels of these miRNAs compared with their expression levels in pAGMK cells, the non-tumorigenic CV-1 cells were the closest and the tumorigenic SF-VERO cells were the most distantly removed from pAGMK cells (the reference point for the non-tumorigenic phenotype). When the <i>in vitro</i> and <i>in vivo</i> characteristics of all cells were superimposed, the trend in miRNA expression in the different AGMK lineages between CV-1 and SF-VERO cells on the X-axis appears to correlate with both the passage level in tissue culture and the evolution of the neoplastic process. The phenotypes were deduced from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014416#pone-0014416-t001" target="_blank">Table 1</a> and the data from the invasion assays.</p

Growth rates and tumorigenic characteristics of the cell lines used for miRNA studies.

1<p>Cells (5×10⁴) were seeded in 60-mm dishes, and viable cell numbers were determined in triplicate by trypan-blue exclusion after 16, 24, 48, and 72 h. Cell-doubling times were estimated from growth-curve plots.</p>2<p>Observation period was 1 year in adult and newborn nude mice.</p>3<p>Newborns have been observed for >8 months and adults have been observed for 1 year.</p><p>ND: not determined.</p

Hierarchical clustering of miRNA expression.

<p>miRNAs included in the heat-map had a fold change >±2 and were significantly expressed (<i>p</i><0.01). Each row shows the relative expression level for a single miRNA and each column represents miRNA profiles of the average triplicate array data: (1) pAGMK cells; (2) 10-87 LP cells; (3) 10-87 HP cells; (4) 10-87 T cells. The red or green color indicates relative high or low expression, respectively. Expression clusters representing different patterns of up-regulation to down-regulation are depicted by Roman numerals on the right hand side of the Figure.</p

Correlations of over-expression of miRNA with migration and invasive phenotypes.

<p>(A) Wound-healing assay of stably expressing miR-376a, miR376abc (a polycistronic cluster of miR-376a, miR-376b and miR-376c), miR-299-5p or miR-negative and 10-87 LP cells; (B) Matrigel-invasion assay with stably expressing miR-376a, miR-376abc, miR-299-5p or miR-negative 10-87 HP cells. 10-87 LP cells were used as the control in the computation of the invasion index.</p

Differentially expressed mature miRNAs in low-passage and high-passage VERO cells in comparison with pAGMK cells.

<p>FC: Fold change (log₂) over pAGMK cells.</p>a<p>only miRNAs that meet the criteria of being at least 4-fold up- or down-regulated with <i>p</i><0.01.</p