Mechanisms of pelvic floor muscle function and the effect on the urethra during a cough

Background: Current measurement tools have difficulty identifying the automaticphysiologic processes maintaining continence, and many questions still remainabout pelvic floor muscle (PFM) function during automatic events.Objective: To perform a feasibility study to characterise the displacement, velocity,and acceleration of the PFM and the urethra during a cough.Design, setting, and participants: A volunteer convenience sample of 23 continentwomen and 9 women with stress urinary incontinence (SUI) from the generalcommunity of San Francisco Bay Area was studied.Measurements: Methods included perineal ultrasound imaging, motion trackingof the urogenital structures, and digital vaginal examination. Statistical analysisused one-tailed unpaired student t tests, and Welch’s correction was applied whenvariances were unequal.Results and limitations: The cough reflex activated the PFM of continent women tocompress the urogenital structures towards the pubic symphysis, which wasabsent in women with SUI. The maximum accelerations that acted on the PFMduring a cough were generally more similar than the velocities and displacements.The urethras of women with SUI were exposed to uncontrolled transverse accelerationand were displaced more than twice as far ( p = 0.0002), with almost twicethe velocity ( p = 0.0015) of the urethras of continent women. Caution regardingthe generalisability of this study is warranted due to the small number of women inthe SUI group and the significant difference in parity between groups.Conclusions: During a cough, normal PFM function produces timely compressionof the pelvic floor and additional external support to the urethra, reducing displacement,velocity, and acceleration. In women with SUI, who have weakerurethral attachments, this shortening contraction does not occur; consequently,the urethras of women with SUI move further and faster for a longer duratio

General Aviation Land Use Planning

In this session we discuss the value of land use planning sessions in determining airside and landside short-, mid-, and long-term needs of the airport. Land use plans have been used to secure funding and increase commercial landside and military airside growth. Currently a plan is being used to develop the first general aviation aerotropolis concept. Join us to learn how a land use plan can increase the marketability and revenue generation of your airport

Metabolic and cardiac adaptation to chronic pharmacologic blockade of facilitative glucose transport in murine dilated cardiomyopathy and myocardial ischemia

Abstract GLUT transgenic and knockout mice have provided valuable insight into the role of facilitative glucose transporters (GLUTs) in cardiovascular and metabolic disease, but compensatory physiological changes can hinder interpretation of these models. To determine whether adaptations occur in response to GLUT inhibition in the failing adult heart, we chronically treated TG9 mice, a transgenic model of dilated cardiomyopathy and heart failure, with the GLUT inhibitor ritonavir. Glucose tolerance was significantly improved with chronic treatment and correlated with decreased adipose tissue retinol binding protein 4 (RBP4) and resistin. A modest improvement in lifespan was associated with decreased cardiomyocyte brain natriuretic peptide (BNP) expression, a marker of heart failure severity. GLUT1 and −12 protein expression was significantly increased in left ventricular (LV) myocardium in ritonavir-treated animals. Supporting a switch from fatty acid to glucose utilization in these tissues, fatty acid transporter CD36 and fatty acid transcriptional regulator peroxisome proliferator-activated receptor α (PPARα) mRNA were also decreased in LV and soleus muscle. Chronic ritonavir also increased cardiac output and dV/dt-d in C57Bl/6 mice following ischemia-reperfusion injury. Taken together, these data demonstrate compensatory metabolic adaptation in response to chronic GLUT blockade as a means to evade deleterious changes in the failing heart

Understanding 5th Grade Students Perspective of STEAM through Sketches

Creative thinking is becoming a significant element of students’ learning to prepare them for today’s complex work environments (Battelle for kids, 2019). While the ideas of science, technology, engineering, art, and mathematics have led to incredible advancements to the global economy, incorporating design thinking can help foster creative thinking in our educational environment. The purpose of this qualitative study was to understand fifth grade students’ perspectives of STEAM within a local school district. Data was collected from participants through a task administered by the STEAM teachers for two fifth grade classes. This task contained 5 separate sections, with room for students to illustrate their image of each element of STEAM, as well as to answer two questions as to what they illustrated and why they illustrated it. By analyzing the data for these fifth grade classes, the school district can look deeper into how students are expressing and understanding STEAM.https://orb.binghamton.edu/research_days_posters_2023/1073/thumbnail.jp

Up, Down and Reciprocal: The Dynamics of Intergenerational Transfers, Family Structure and Health in a Low-Income Context

In the absence of well-functioning public transfer systems and safety nets, the family acts as the key provider of income and support through the intergenerational redistribution of resources. In this paper we use micro-level longitudinal data and a mix of methodologies to document the lifecycle patterns of financial transfers in a rural, sub-Saharan African population. Underneath a well-established age-pattern of intergenerational transfers in which transfer patterns change according to broad stages of the economic life cycle, our analyses document significant heterogeneity and fluidity: Intergenerational transfers are variable and reverse their direction, with individuals moving between the provider and recipient states repeatedly across their life course and within each major stage of the life-cycle. Contrary to common perceptions about family transfers ameliorating short-term shocks, transfers in our analyses are driven primarily by demographic factors such as changes in health, household size, and household composition, rather than short-term events. Overall our analyses suggest that the role of transfers in this rural sub-Saharan context is significantly more complex than suggested by theories and evidence on aggregate transfer patterns, and at the micro-level, intergenerational transfers encapsulate multiple functions ranging from direct exchange to old-age support in the absence of a public pension system

Opioids alter paw placement during walking, confounding assessment of analgesic efficacy in a postsurgical pain model in mice

Introduction: Hind paw-directed assays are commonly used to study the analgesic effects of opioids in mice. However, opioid-induced hyperlocomotion can obscure results of such assays. Objectives: We aimed to overcome this potential confound by using gait analysis to observe hind paw usage during walking in mice. Methods: We measured changes in the paw print area after induction of postsurgical pain (using the paw incision model) and treatment with oxycodone. Results: Paw incision surgery reduced the paw print area of the injured hind paw as mice avoided placing the incised section of the paw on the floor. Surprisingly, oxycodone caused a tiptoe-like gait in mice, reducing the paw print area of both hind paws. Further investigation of this opioid-induced phenotype revealed that analgesic doses of oxycodone or morphine dose-dependently reduced the hind paw print area in uninjured mice. The gait changes were not dependent on opioid-induced increases in the locomotor activity; speed and paw print area had no correlation in opioid-treated mice, and other analgesic compounds that alter locomotor activity did not affect the paw print area. Conclusion: Unfortunately, the opioid-induced tiptoe gait phenotype prevented gait analysis from being a viable metric for demonstrating opioid analgesia in injured mice. However, this work reveals an important, previously uncharacterized effect of treatment with analgesic doses of opioids on paw placement. Our characterization of how opioids affect gait has important implications for the use of mice to study opioid pharmacology and suggests that scientists should use caution when using hind paw-directed nociceptive assays to test opioid analgesia in mice

The formation of tephra layers in peatlands: An experimental approach

Tephrochronology provides a valuable method of dating peat deposits but results may be compromised if tephra undergoes significant post-depositional movement. This study takes an experimental approach to investigate the processes of tephra taphonomy. Tephra was applied to peats and movement monitored over periods of up to 6 years. Experiments combined field studies on six British peatlands with rainfall simulation experiments in the laboratory. Tephra moved up to 15 cm down through the peat but the vast majority remained at the surface at time of deposition, forming a layer which accurately recorded the palaeo-surface. Tephra moved both down, by shards sinking through the peat, and up, with shards probably being moved by plant growth or with water table variability. The extent of tephra movement most likely depends on the density and porosity of the surface peat; there is no simple relationship with wetness. There is some indication that the extent of tephra movement depends on the tephra particle size but this will require further work to confirm. The taphonomy of tephra is an important issue which should be considered in all tephrochronology studies in peatlands

A novel FRET-based screen in high-throughput format to identify inhibitors of malarial and human glucose transporters

The glucose transporter PfHT is essential to the survival of the malaria parasite Plasmodium falciparum and has been shown to be a druggable target with high potential for pharmacological intervention. Identification of compounds against novel drug targets is crucial to combating resistance against current therapeutics. Here, we describe the development of a cell-based assay system readily adaptable to high-throughput screening that directly measures compound effects on PfHT-mediated glucose transport. Intracellular glucose concentrations are detected using a genetically encoded fluorescence resonance energy transfer (FRET)-based glucose sensor. This allows assessment of the ability of small molecules to inhibit glucose uptake with high accuracy (Z′ factor of >0.8), thereby eliminating the need for radiolabeled substrates. Furthermore, we have adapted this assay to counterscreen PfHT hits against the human orthologues GLUT1, -2, -3, and -4. We report the identification of several hits after screening the Medicines for Malaria Venture (MMV) Malaria Box, a library of 400 compounds known to inhibit erythrocytic development of P. falciparum. Hit compounds were characterized by determining the half-maximal inhibitory concentration (IC(50)) for the uptake of radiolabeled glucose into isolated P. falciparum parasites. One of our hits, compound MMV009085, shows high potency and orthologue selectivity, thereby successfully validating our assay for antimalarial screening

A refined rat primary neonatal microglial culture method that reduces time, cost and animal use

We thank the Development Trust of The University of Aberdeen and the Scottish Rugby Union for their financial support. We also thank Dr Elizabeth Old from Wolfson Centre for Age-related Diseases, King’s College London for providing technical training with the previous protocol and helping in the editorial process of this manuscript.Peer reviewedPostprin

GLUT4, GLUT1, and GLUT8 are the dominant GLUT transcripts expressed in the murine left ventricle

BACKGROUND: The heart derives energy from a wide variety of substrates including fatty acids, carbohydrates, ketones, and amino acids. The healthy heart generates up to 30% of its ATP from glucose. Under conditions of cardiac injury or stress, the heart relies even more heavily on glucose as a source of fuel. Glucose is transported into the heart by members of the family of facilitative glucose transporters (GLUTs). While research examining the transport of glucose into the heart has primarily focused on the roles of the classical glucose transporters GLUT1 and GLUT4, little is known about the functions of more newly identified GLUT isoforms in the myocardium. METHODS: In this study the presence and relative RNA message abundance of each of the known GLUT isoforms was determined in left ventricular tissue from two commonly used inbred laboratory mouse strains (C57BL/6J and FVB/NJ) by quantitative real time PCR. Relative message abundance was also determined in GLUT4 null mice and in murine models of dilated and hypertrophic cardiomyopathy. RESULTS: GLUT4, GLUT1, and GLUT8 were found to be the most abundant GLUT transcripts in the normal heart, while GLUT3, GLUT10, and GLUT12 are present at relatively lower levels. Assessment of relative GLUT expression in left ventricular myocardium from mice with dilated cardiomyopathy revealed increased expression of GLUT1 with reduced levels of GLUT4, GLUT8, and GLUT12. Compensatory increase in the expression of GLUT12 was observed in genetically altered mice lacking GLUT4. CONCLUSIONS: Glucose transporter expression varies significantly among murine models of cardiac dysfunction and involves several of the class III GLUT isoforms. Understanding how these more newly identified GLUT isoforms contribute to regulating myocardial glucose transport will enhance our comprehension of the normal physiology and pathophysiology of the heart