Saúde bucal: uma revisão crítica sobre programações educativas para escolares

Em saúde bucal, a situação epidemiológica brasileira ainda é grave devido às condições sociais e econômicas da população, à pequena parcela de investimentos que a área recebe em relação ao total do SUS e à falta de informação sobre os cuidados básicos de saúde. Embora a odontologia se mostre muito desenvolvida em tecnologia, não responde em níveis significativos às demandas dos problemas de saúde bucal da população. Nesse contexto, a educação em saúde bucal tem sido cada vez mais requisitada, considerando o baixo custo e as possibilidades de impacto odontológico no âmbito público e coletivo. A importância de práticas preventivas e educativas em saúde bucal nos levou a realizar este estudo, apontando diferentes programas odontológicos, mediante estudo de revisão, para analisá-los em suas propostas, metodologias, possibilidades e limitações, visando refletir sobre o tema com foco nos aspectos educativos que ainda desafiam os programas de saúde bucal. Identificaram-se quatro tendências nos programas analisados. O estudo apontou para a necessidade de se repensar as práticas educativas.The Brazilian epidemiological situation in oral health is still serious due to inappropriate social and economic conditions of the population; the small amount of investment compared to the total amount of SUS; and lack of information about basic health cares. Although the dentistry sector seems to be very developed regarding technology, it does not meet significantly the population demand for oral health. In accordance to this fact, oral health education has been required every day more, with regard to the low costs and the possibilities of odontologycal impact on public and collective scope. The main reason for this study was the importance of preventive and educative actions in oral health, aiming different odontologycal programmes by means of review studies, to analyse their proposals, methodologies, possibilities and limitations, seeking to consider the theme focusing in educative aspects that still challenge oral health programmes. In the analysed programmes were identified four tendencies. The study pointed out the necessity of rethink the educative actions

BRCA1 and BRCA2 rearrangements in Brazilian individuals with Hereditary Breast and Ovarian Cancer Syndrome

Abstract Approximately 5-10% of breast cancers are caused by germline mutations in high penetrance predisposition genes. Among these, BRCA1 and BRCA2, which are associated with the Hereditary Breast and Ovarian Cancer (HBOC) syndrome, are the most frequently affected genes. Recent studies confirm that gene rearrangements, especially in BRCA1, are responsible for a significant proportion of mutations in certain populations. In this study we determined the prevalence of BRCA rearrangements in 145 unrelated Brazilian individuals at risk for HBOC syndrome who had not been previously tested for BRCA mutations. Using Multiplex Ligation-dependent Probe Amplification (MLPA) and a specific PCR-based protocol to identify a Portuguese founder BRCA2 mutation, we identified two (1,4%) individuals with germline BRCA1 rearrangements (c.547+240_5193+178del and c.4675+467_5075-990del) and three probands with the c.156_157insAlu founder BRCA2 rearrangement. Furthermore, two families with false positive MLPA results were shown to carry a deleterious point mutation at the probe binding site. This study comprises the largest Brazilian series of HBOC families tested for BRCA1 and BRCA2 rearrangements to date and includes patients from three regions of the country. The overall observed rearrangement frequency of 3.44% indicates that rearrangements are relatively uncommon in the admixed population of Brazil

BRCA1 and BRCA2 rearrahgements in brazilian individuals with hereditary breast and ovarian cancer syndrome

Approximately 5-10% of breast cancers are caused by germline mutations in high penetrance predisposition genes. Among these, BRCA1 and BRCA2, which are associated with the Hereditary Breast and Ovarian Cancer (HBOC) syndrome, are the most frequently affected genes. Recent studies confirm that gene rearrangements, especially in BRCA1, are responsible for a significant proportion of mutations in certain populations. In this study we determined the prevalence of BRCA rearrangements in 145 unrelated Brazilian individuals at risk for HBOC syndrome who had not been previously tested forBRCA mutations. Using Multiplex Ligation-dependent Probe Amplification (MLPA) and a specific PCR-based protocol to identify a Portuguese founder BRCA2 mutation, we identified two (1,4%) individuals with germline BRCA1 rearrangements (c.547+240_5193+178del and c.4675+467_5075-990del) and three probands with the c.156_157insAlu founder BRCA2 rearrangement. Furthermore, two families with false positive MLPA results were shown to carry a deleterious point mutation at the probe binding site. This study comprises the largest Brazilian series of HBOC families tested for BRCA1 and BRCA2 rearrangements to date and includes patients from three regions of the country. The overall observed rearrangement frequency of 3.44% indicates that rearrangements are relatively uncommon in the admixed population of Brazil

BRCA1 and BRCA2 rearrahgements in brazilian individuals with hereditary breast and ovarian cancer syndrome

Approximately 5-10% of breast cancers are caused by germline mutations in high penetrance predisposition genes. Among these, BRCA1 and BRCA2, which are associated with the Hereditary Breast and Ovarian Cancer (HBOC) syndrome, are the most frequently affected genes. Recent studies confirm that gene rearrangements, especially in BRCA1, are responsible for a significant proportion of mutations in certain populations. In this study we determined the prevalence of BRCA rearrangements in 145 unrelated Brazilian individuals at risk for HBOC syndrome who had not been previously tested forBRCA mutations. Using Multiplex Ligation-dependent Probe Amplification (MLPA) and a specific PCR-based protocol to identify a Portuguese founder BRCA2 mutation, we identified two (1,4%) individuals with germline BRCA1 rearrangements (c.547+240_5193+178del and c.4675+467_5075-990del) and three probands with the c.156_157insAlu founder BRCA2 rearrangement. Furthermore, two families with false positive MLPA results were shown to carry a deleterious point mutation at the probe binding site. This study comprises the largest Brazilian series of HBOC families tested for BRCA1 and BRCA2 rearrangements to date and includes patients from three regions of the country. The overall observed rearrangement frequency of 3.44% indicates that rearrangements are relatively uncommon in the admixed population of Brazil