Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution

The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm ) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter

Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution

The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm ) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter

A Redox-Probe-Free Immunosensor Based on Electrocatalytic Prussian Blue Nanostructured Film One-Step-Prepared for Zika Virus Diagnosis

The Zika virus (ZIKV) is a great concern for global health due to its high transmission, including disseminating through blood, saliva, urine, semen and vertical transmission. In some cases, ZIKV has been associated with microcephaly, neurological disorders, and Guillain–Barré syndrome. There is no vaccine, and controlling the disease is a challenge, especially with the co-circulation of the Dengue virus, which causes a severe cross-reaction due to the similarity between the two arboviruses. Considering that electrochemical immunosensors are well-established, sensitive, and practical tools for diagnosis, in this study we developed a sensor platform with intrinsic redox activity that facilitates measurement readouts. Prussian blue (PB) has a great ability to form electrocatalytic surfaces, dispensing redox probe solutions in voltammetric measurements. Herein, PB was incorporated into a chitosan–carbon nanotube hybrid, forming a nanocomposite that was drop-casted on a screen-printed electrode (SPE). The immunosensor detected the envelope protein of ZIKV in a linear range of 0.25 to 1.75 µg/mL (n = 8, p < 0.01), with a 0.20 µg/mL limit of detection. The developed immunosensor represents a new method for electrochemical measurements without additional redox probe solutions, and it is feasible for application in point-of-care diagnosis

Antibacterial properties of contact defensive secretions in Neotropical Crematogaster ants (Hymenoptera: Formicidae)

info:eu-repo/semantics/publishe

Post-pandemic influenza A (H1N1) 2009 virus infection in pregnant women in Ceará, Brazil

Submitted by sandra infurna ([email protected]) on 2016-04-30T17:34:01Z No. of bitstreams: 1 marilda3_siqueira_etal_IOC_2015.pdf: 102162 bytes, checksum: 5845677cc9b662023ef10385ba5be116 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-04-30T17:52:51Z (GMT) No. of bitstreams: 1 marilda3_siqueira_etal_IOC_2015.pdf: 102162 bytes, checksum: 5845677cc9b662023ef10385ba5be116 (MD5)Made available in DSpace on 2016-04-30T17:52:51Z (GMT). No. of bitstreams: 1 marilda3_siqueira_etal_IOC_2015.pdf: 102162 bytes, checksum: 5845677cc9b662023ef10385ba5be116 (MD5) Previous issue date: 2015Laboratório Central de Saúde Pública do Ceará. Fortaleza, CE, Brasil / Universidade Estadual do Ceará. Rede de Biotecnologia do Nordeste. Fortaleza, CE, Brasil / Centro Universitário Christus - Unichristus. Fortaleza, CE, Brasil.Laboratório Central de Saúde Pública do Ceará. Fortaleza, CE, Brasil / Secretaria de Saúde do Estado do Ceará. Fortaleza, CE, Brasil.Laboratório Central de Saúde Pública do Ceará. Fortaleza, CE, Brasil / Secretaria de Saúde do Estado do Ceará. Fortaleza, CE, Brasil.Secretaria de Saúde do Estado do Ceará. Fortaleza, CE, Brasil / Centro Universitário Christus - Unichristus. Fortaleza, CE, Brasil.Universidade Federal do Ceará. CE, Brasil.Laboratório Central de Saúde Pública do Ceará. Fortaleza, CE, Brasil / Secretaria de Saúde do Estado do Ceará. Fortaleza, CE, Brasil / Universidade Estadual do Ceará. Rede de Biotecnologia do Nordeste. Fortaleza, CE, Brasil.Universidade de Fortaleza - UNIFOR. Fortaleza, CE, Brasil.Centro Universitário Christus - Unichristus. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Ceará. Rede de Biotecnologia do Nordeste. Fortaleza, CE, Brasil.Objective The aim of this study was to present results of the postpandemic phase of A(H1N1)pdm09 virus infection in pregnant women in Cear a, Brazil, during the January–June 2012 influenza season. Results One hundred and fifty-four nasopharyngeal swab samples were collected from pregnant women admitted to hospitals with suspected severe acute respiratory infection (SARI). Fifty-three (34 4%) had laboratory-confirmed A(H1N1)pdm09 virus infection with 15 (28 3%) outpatients and 38 (71 7%) hospitalized. Five (9 4%) women were in the first trimester of pregnancy, 20 (37 7%) in the second trimester of pregnancy, and 24 (45 2%) in the third trimester of pregnancy. Three had no information about the time of pregnancy. Six samples from newborns were also analyzed, of which three were nasopharyngeal swab positive for A(H1N1)pdm09. These swabs were collected immediately after birth, with the exception of one that was collected on the day after birth. Conclusion Our findings suggest that transplacental transfer of influenza viruses could occur as a result of severe illness in pregnancy. It is therefore important to encourage women to be vaccinated against influenza in order to avoid pregnancy complications

Evaluation of antioxidant, toxicological and anxiolytic-like effect of ethanolic extracts of Ziziphus cotinifolia Reissek in adult zebrafish (Danio rerio)

Background: The GABAergic and serotoninergic neurotransmission pathways are involved in the control of anxiety and ensure emotional balance. Some plant species have substances with anxiolytic activity, a therapeutic effect associated with the presence of phenolic compounds. Methods: Before assessing the existence of anxiolytic activity, ethanolic extracts from leaves, branches and roots of Ziziphus cotinifolia Reissek were evaluated regarding biological activity and absence of toxicity. Later the influences of the samples on locomotor and anxiolytic activity, anxiolytic pathways and probable mechanisms of neurotransmission were evaluated. Results: The EEtFJ extract (0.5 mg/mL) did not have its anxiolytic activity reversed by the GABAergic receptor antagonist (Fmz + EEtFJ = EEjFJ; p<0.05). The twig extracts and flumazenil only partially reduced the time spent in the light zone (Fmz + EEtGJ < + EEtGJ; p<0.05), the root extract was not reversed by flumazenil (Fmz + EEtFRJ = EEtFRJ). Conclusion: The initial analysis data reveal the presence of phenolic compounds that explain the biological activities found and, as they act on different targets of neurotransmission systems involved in the control of anxiety disorders, they have potential for the production of herbal medicines with synergistic action