SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION : INTERACTION BETWEEN TWO TYPES OF THYMUS-DEPENDENT CELLS

The present studies have shown that two subpopulations of thymus-dependent lymphocytes may act synergistically in the mixed lymphocyte reaction (MLR) in the mouse. One subpopulation was well represented in the young adult thymus and the other in lymph nodes. For optimum synergy, both populations must be allogeneic to the stimulator cells. Pretreatment of either population with mitomycin-C abolished synergy. Anti-θ serum abolished both MLR responding and synergizing activities of lymphoid cells. The two thymus-dependent subpopulations were both present in the spleen, and displayed different migratory patterns when injected into irradiated mice: one population went to spleens of the irradiated mice, the other to lymph nodes. The effects of anti-thymocyte serum on the MLR and upon synergy were assessed. While minor differences exist and are herein described, our overall results strongly suggest that in our experiments with synergy in MLR, we may be dealing with the same T1- and T2-cell subpopulations described by Cantor and Asofsky and coworkers (1, 2, 4, 5, 14) as displaying synergy in the graft-vs.-host reaction

HYPERTENSION AND HISTOCOMPATIBILITY ANTIGENS - RESPONSE

UNIV SAO PAULO,FAC MED,INST CORACAO,SAO PAULO,BRAZILWeb of Scienc

Tetragametic chimerism identified during routine histocompatibility testing

Universidade Federal de São Paulo, Immunogenet Div, São Paulo, BrazilUniversidade Federal de São Paulo, Immunogenet Div, São Paulo, BrazilWeb of Scienc

DTT treatment of the serum may disclose the presence of anti-HLA antibodies in ELISA-PRA assay

Universidade Federal de São Paulo, Div Immunogenet, São Paulo, BrazilUniversidade Federal de São Paulo, Div Immunogenet, São Paulo, BrazilWeb of Scienc