Time course regulation of cell death–related proteins in naïve and optic nerve injured retinas

Western blot time course analyses showing the regulation of tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), total Caspase 3, active Caspase 3, and c-fos in naïve, intraorbital nerve transection (IONT)-, and intraorbital nerve crush (IONC)-injured retinas. Graphs show quantification of protein signals (n=4 animals per lesion and time point, western blots were replicated three times). The signal from injured retinas is referred to the naïve signal, which was arbitrarily considered 100%. To verify the amount of loaded protein, western blots were incubated with β-actin (an example is shown). Error bars show the standard error of the mean (SEM) for each experiment.<p><b>Copyright information:</b></p><p>Taken from "Time course profiling of the retinal transcriptome after optic nerve transection and optic nerve crush"</p><p></p><p>Molecular Vision 2008;14():1050-1063.</p><p>Published online 03 Jun 2008</p><p>PMCID:PMC2426719.</p><p></p

Expression pattern of cell death–related proteins in naïve and optic nerve injured retinas

Immunohistofluorescence analyses for tumor necrosis factor receptor superfamily member 1a (TNFR1a), tumor necrosis factor receptor type 1, associated death domain (TRADD), Caspase 3, and c-fos to fluorogold-traced retinas in naïve, intraorbital nerve transection (IONT) and intraorbital nerve crush (IONC) injured retinas. Expression pattern of TNFR1a (red signal) in naïve retinas (, right), IONT-injured retinas (, right) and IONC-injured retinas (, right). Left images are the corresponding fluorogold (FG) images (blue signal). Expression pattern of TRADD (red signal) in naïve retinas (, right), IONT-injured retinas (, right) and IONC-injured retinas (, right). Left images are the corresponding fluorogold (FG) images (blue signal). Expression pattern of Caspase 3 (red signal) in naïve retinas (, right), IONT-injured retinas (, right) and IONC-injured retinas (, right). Left images are the corresponding fluorogold (FG) images (blue signal). Expression pattern of c-fos (red signal) in naïve retinas (, right), IONT-injured retinas (, right) and IONC-injured retinas (, right). Left images are the corresponding fluorogold (FG) images (blue signal). Magnifications in squares show the co-localization of a given protein with FG-labeled retinal ganglion cells (RGCs). Arrows point to RGC, arrowheads indicate the outer nuclear layer and asterisks mark the nerve layer.<p><b>Copyright information:</b></p><p>Taken from "Time course profiling of the retinal transcriptome after optic nerve transection and optic nerve crush"</p><p></p><p>Molecular Vision 2008;14():1050-1063.</p><p>Published online 03 Jun 2008</p><p>PMCID:PMC2426719.</p><p></p