Transfer of chemical elements from milk to dairy products

Abstract A pilot study was carried out to evaluate the transfer of As, Cd, Pb, Ni, Cr and Hg from milk to dairy products and to correlate their behavior with that of some components and macro-elements of milk. Due to the low level of these chemical elements in most common commercial products, hard cheese was produced starting from cow's milk spiked with the analytes of interest. Several intermediate and final products coming from cheese making were sampled and analysed for content of fat, dry matter, proteins, macro and oligo-elements. The relationship between spiked elements and milk components was evaluated through both the study of concentration factors and the statistical analysis (Principal Component Analysis and correlation matrix). Except for As and Hg, a clear correlation between spiked elements and milk components was found so a likely bond with proteins, fat and dry matter was demonstrated. As for Pb, for which a legal limit (Maximum Level, ML) is set in the pertinent European regulations, it was found that the link with proteins could lead to an increase of this element concentration different from the mere concentration factor from milk to cheese. Furthermore, it was proven that the stage of ripening affected the variation of Pb concentration so this aspect should be deeply considered in case of setting a ML in cheese or extrapolating a ML from milk to cheese

proficiency testing as a tool to assess quality of data the experience of the eu reference laboratory for chemical elements in food of animal origin

AbstractQuality and reliability of analytical results are, in general, key issues for all laboratories but become a top priority for laboratories accredited according to ISO/IEC 17025:2005. In this international standard the proficiency testing (PT) is regarded as a means to assure the validity of results. Nowadays, the proved competence of laboratories is an essential requirement especially for that structures that are involved in the official controls aimed at ensuring the safety of EU food products and the public health. To guarantee the EU consumers, the Council and the Commission have designated 28 European Union Reference Laboratories (EURLs) for food and feed, whose main role is to contribute to the standardization of analytical methods and to the harmonization of performance among the EU National Reference Laboratories (NRLs) to reach a comparable level of quality in the analytical data among all Member States. With this aim, the organization of PTs is a task that each EURL has to accomplish. Over the last 15 years, the EURL for chemical elements in food of animal origin (EURL-CEFAO) have organized 32 PTs on determination of total As, Cd, Pb and total Hg in meat, milk, fish and offal for the benefit of its network of NRLs. Some specific aspects of this activity will be discussed (e.g. preparation and characterization of PT materials, statistical evaluation of data, follow-up actions). Finally, based on the EURL-CEFAO experience, it will be demonstrated that the participation into PTs on a regular basis can result in an improvement of the laboratory's performance as well as in the harmonization of the results submitted by participants

Blood thiamine, zinc, selenium, lead and oxidative stress in a population of male and female alcoholics: clinical evidence and gender differences

Introduction. Long term alcohol abuse is associated with deficiencies in essential nutrients and minerals that can cause a variety of medical consequences including accumulation of toxic metals. Aim. The aim of this research is to get evidence-based data to evaluate alcohol damage and to optimize treatment. Thiamine and thiamine diphosphate (T/TDP), zinc (Zn), selenium (Se), lead (Pb) and oxidative stress in terms of reactive oxygen metabolites (ROMs) were examined in blood samples from 58 alcohol dependent patients (17 females and 41 males). Results. T/TDP concentration in alcoholics resulted significantly lower than controls (p < 0.005) for both sexes. Serum Zn and Se did not significantly differ from reference values. Levels of blood Pb in alcoholics resulted significantly higher (p < 0.0001) than Italian reference values and were higher in females than in males. ROMs concentration was significantly higher than healthy population only in female abusers (p = 0.005). Conclusion. Alcoholics show a significant increase in blood oxidative stress and Pb and decrease in thiamine. Impairment occurs mainly in female abusers confirming a gender specific vulnerability

Determination of total silicon and SiO2 particles using an ICP-MS based analytical platform for toxicokinetic studies of synthetic amorphous silica

Synthetic amorphous silica (SAS), manufactured in pyrogenic or precipitated form, is a nanomaterial with a widespread use as food additive (E 551). Oral exposure to SAS results from its use in food and dietary supplements, pharmaceuticals and toothpaste. Recent evidence suggests that oral exposure to SAS may pose health risks and highlights the need to address the toxic potential of SAS as affected by the physicochemical characteristics of the different forms of SAS. For this aim, investigating SAS toxicokinetics is of crucial importance and an analytical strategy for such an undertaking is presented. The minimization of silicon background in tissues, control of contamination (including silicon release from equipment), high-throughput sample treatment, elimination of spectral interferences affecting inductively coupled plasma mass spectrometry (ICP-MS) silicon detection, and development of analytical quality control tools are the cornerstones of this strategy. A validated method combining sample digestion with silicon determination by reaction cell ICP-MS is presented. Silica particles are converted to soluble silicon by microwave dissolution with mixtures of HNO3, H2O2 and hydrofluoric acid (HF), whereas interference-free ICP-MS detection of total silicon is achieved by ion-molecule chemistry with limits of detection (LoDs) in the range 0.2–0.5 µg Si g−1 for most tissues. Deposition of particulate SiO2 in tissues is assessed by single particle ICP-MS105Nanotechnology in Agriculture and Food IndustryThis work arises from the NANOGENOTOX Joint Action which has received funding from the European Union, in the framework of the Health Programme. The NANOGENOTOX Joint Action was co-funded by the Executive Agency for Health and Consumers (Grant Agreement 2009 21 01). This publication reflects only the authors’ views and the Executive Agency for Health and Consumers (now CHAFEA) is not liable for any use that may be made of the information contained therei