Human Cep192 Is Required for Mitotic Centrosome and Spindle Assembly

SummaryAs cells enter mitosis, centrosomes dramatically increase in size and ability to nucleate microtubules. This process, termed centrosome maturation, is driven by the accumulation and activation of γ-tubulin and other proteins that form the pericentriolar material on centrosomes during G2/prophase. Here, we show that the human centrosomal protein, Cep192 (centrosomal protein of 192 kDa), is an essential component of the maturation machinery. Specifically, we have found that siRNA depletion of Cep192 results in a complete loss of functional centrosomes in mitotic but not interphase cells. In mitotic cells lacking Cep192, microtubules become organized around chromosomes but rarely acquire stable bipolar configurations. These cells contain normal numbers of centrioles but cannot assemble γ-tubulin, pericentrin, or other pericentriolar proteins into an organized PCM. Alternatively, overexpression of Cep192 results in the formation of multiple, extracentriolar foci of γ-tubulin and pericentrin. Together, our findings support the hypothesis that Cep192 stimulates the formation of the scaffolding upon which γ-tubulin ring complexes and other proteins involved in microtubule nucleation and spindle assembly become functional during mitosis

Whole genome functional analysis identifies novel components required for mitotic spindle integrity in human cells

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Cep192 Controls the Balance of Centrosome and Non-Centrosomal Microtubules during Interphase

<div><p>Cep192 is a centrosomal protein that contributes to the formation and function of the mitotic spindle in mammalian cells. Cep192’s mitotic activities stem largely from its role in the recruitment to the centrosome of numerous additional proteins such as gamma-tubulin and Pericentrin. Here, we examine Cep192’s function in interphase cells. Our data indicate that, as in mitosis, Cep192 stimulates the nucleation of centrosomal microtubules thereby regulating the morphology of interphase microtubule arrays. Interestingly, however, cells lacking Cep192 remain capable of generating normal levels of MTs as the loss of centrosomal microtubules is augmented by MT nucleation from other sites, most notably the Golgi apparatus. The depletion of Cep192 results in a significant decrease in the level of centrosome-associated gamma-tubulin, likely explaining its impact on centrosome microtubule nucleation. However, in stark contrast to mitosis, Cep192 appears to maintain an antagonistic relationship with Pericentrin at interphase centrosomes. Interphase cells depleted of Cep192 display significantly higher levels of centrosome-associated Pericentrin while overexpression of Cep192 reduces the levels of centrosomal Pericentrin. Conversely, depletion of Pericentrin results in elevated levels of centrosomal Cep192 and enhances microtubule nucleation at centrosomes, at least during interphase. Finally, we show that depletion of Cep192 negatively impacts cell motility and alters normal cell polarization. Our current working hypothesis is that the microtubule nucleating capacity of the interphase centrosome is determined by an antagonistic balance of Cep192, which promotes nucleation, and Pericentrin, which inhibits nucleation. This in turn determines the relative abundance of centrosomal and non-centrosomal microtubules that tune cell movement and shape.</p></div

Cep192 is required for efficient polarization and cell migration.

<p>A) Phase-contrast images from a 2-D scratch assay performed on control Cep192 siRNA treated U2OS cells. U2OS cells were plated into Ibidi Culture-Insert dishes following siRNA treatment and the cell-free zone was generated by removing the insert. B) Quantification of the efficiency at which control and Cep192 siRNA-treated cells moved into the cell-free zone (determined by measuring the cell free area at the indicated timepoint). Vertical bars represent S.E.M. P<0.0001. N = 3 experiments per condition.</p

Cep192 is required for the assembly of the interphase centrosome.

<p>A) Graph quantifying the effects of Cep192 siRNA on the levels of centrosome-associated PCM proteins as determined by quantitative immunofluorescence. P values for all experiments are ≤0.0003. S.E.M. is depicted as vertical bars. N≥23 cells per experiment from 3 independent experiments. B) Representative immunofluorescence micrographs showing the alterations in PCM protein staining levels quantified in A.</p

Cep192 impacts cell polarization.

<p>A) Top panels show immunofluorescence micrographs of control and Cep192 siRNA-treated WM266-4 cells stained for alpha-tubulin 72 hours after siRNA treatment. Cep192 siRNA-treated WM266-4 cells are clearly elongated relative to controls. Bottom panels show the measured average axial ratios (length/width) of control and Cep192 siRNA-treated U2OS cells and WM266-4 melanoma cells. Vertical bars represent S.E.M. P = 0.0007 (U2OS cells) and <0.0001 (WM266-4 cells). N≥20 cells from each condition per experiment from 3 independent experiments.</p

Cep192 is required for normal MT dynamics.

<p>A) Representative images of tracked EB1-GFP comet trajectories during a 30s time interval in control and KD conditions in U2OS cells. Note the lack of a major MT nucleating site in the Cep192 KD cell. B) MT tracks emanate from the centrosome significantly less frequently in Cep192 KD cells relative to control (5.4 comets/centrosome vs. 14.2 (comets/centrosome)). C) Using the automated tracking program, plusTipTracker to track EB1 comets, we found comets in Cep192 depleted cells traveled at a significantly slower rate relative to control (18.4±0.193 µm/min vs. 19.2±0.112µm/min, respectively). D) Despite changes in MT organization, there was no change in the number of comets per cell. Vertical bars represent S.E.M. P values are <0.0001, P = 0.0007, and P = 0.4377, respectively. N≥16 cells per experiment from 3 independent experiments per condition.</p

Inducible Cep192 expression rescues centrosomal arrays in FLAG-Cep192-2 Flp-In T-Rex U2OS cells.

<p>A) Cep192 was successfully depleted using siRNA targeted specifically to the Cep192-1 isoform. Using an inducible U2OS tetracylcine inducible FLAG-Cep192-2 cell line, the shorter exogenous protein, resistant to the siRNA, is expressed at wild type levels. Following normalization to GAPDH, Cep192-2 levels were within 6.6% of controls. B) Representative immunofluorescence micrographs of MT arrangement in each experimental condition. Boxed and magnified regions focus on centrin labeled sites. C) Graph showing the percentage of cells displaying obvious centrosome MT arrays in each condition (24 hours after Cep192-2 induction; 88.5% for control, 46.5% for Cep192 KD, and 78.4% for knockdown induced cells). Vertical bars represent S.D. P<0.0001. N≥10 cells per condition from 3 independent experiments.</p

Cep192 knockdown causes a positive shift in acetylated tubulin density in U2OS cells.

<p>A) Quantitative immunofluorescence of cells immunostained for alpha-tubulin showed no significant shift in total interphase MT polymer mass between control and Cep192 depleted cells. Vertical bars represent S.E.M. P value is 0.5951 N≥20 cells per experiment from 3 independent experiments. B) Immunofluorescence micrographs showing control and Cep192 siRNA-treated U2OS cells double labeled for acetylated tubulin and α-tubulin. C) Western blot control and Cep192 siRNA-treated U2OS cell lysates stained for acetylated tubulin. Densitometry measurements indicated that acetylated tubulin increases ∼64% following Cep192 knockdown. GAPDH is shown as a loading control. D) Levels of tyrosinated tubulin decrease 9% following Cep192 KD.</p