Primer sequences used for quantitative real-time polymerase chain reaction.

<p>¹Amplicon (bp)</p><p>²Annealing Temperature (°C)</p><p>³CTSL2, cathepsin L2; GHR, growth hormone receptor; IGF-I, insulin-like growth factor I; PI3KR1, phosphatidylinositol 3-kinase, regulatory 1.</p><p>Primer sequences used for quantitative real-time polymerase chain reaction.</p

Experimental diets, centesimal composition (expressed as-fed basis).

<p>¹MD, methionine deficient; DL1, recommended level of methionine supplementation; DL2, excess methionine supplementation.</p><p>²Supplied by kilogram of diet: retinyl-acetate, 3.44 mg; cholecalciferol, 50 μg; DL-<i>α-</i>tocopherol, 15 mg; thiamine, 1.63 mg; riboflavin, 4.9 mg; pyridoxine, 3.26 mg; cyanocobalamin, 12 μg; D-pantothenic acid, 9.8 mg; D-biotin, 0.1 mg; menadione, 2.4 mg; folic acid, 0.82 mg; niacinamide, 35 mg; selenium, 0.2 mg; iron, 35 mg; copper, 8 mg; manganese, 60 mg; zinc, 50 mg; I, 1 mg; choline, 650 mg; salinomycin, 60 mg; avilamycin, 5 mg; butyl hydroxy toluene, 80 mg.</p><p>³Feed formulations were based on the total amino acids of corn and soybean meal as analyzed by Evonik Degussa (Hanau, Germany). The digestibility coefficient suggested by Rostagno et al. (2011) was used to obtain the digestible amino acids. AME: apparent metabolizable energy. The amino acids, crude protein and dry matter were analyzed by Evonik Degussa (Hanau, Germany).</p><p>Experimental diets, centesimal composition (expressed as-fed basis).</p

IGF-I, GHR, PI3KR1, CTSL2, and atrogin 1 gene expression of broilers from the starter period.

<p>^{a, b} Mean values within a column with different superscript letters are significantly different (P<0.05).</p><p>¹MD, methionine deficient; DL1, recommended level of methionine supplementation; DL2, methionine supplementation in excess.</p><p>²CTSL2, cathepsin L2; GHR, growth hormone receptor; IGF-I, insulin-like growth factor I; PI3KR1, phosphatidylinositol 3-kinase, regulatory 1.</p><p>³Expressed as arbitrary units (AU).</p><p>NS, non significant.</p><p>IGF-I, GHR, PI3KR1, CTSL2, and atrogin 1 gene expression of broilers from the starter period.</p

Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.

<p>Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.</p

IGF-I, GHR, PI3KR1, CTSL2, and atrogin 1 gene expression of broilers from the grower period.

<p>^{a, b, c, d} Mean values within a column with different superscript letters are significantly different (P<0.05).</p><p>¹MD, methionine deficient; DL1, recommended level of methionine supplementation; DL2, methionine supplementation in excess.</p><p>²CTSL2, cathepsin L2; GHR, growth hormone receptor; IGF-I, insulin-like growth factor I; PI3KR1, phosphatidylinositol 3-kinase, regulatory 1.</p><p>³Expressed as arbitrary units (AU).</p><p>NS, non significant.</p><p>IGF-I, GHR, PI3KR1, CTSL2, and atrogin 1 gene expression of broilers from the grower period.</p

Primer sequences used for quantitative real-time polymerase chain reaction.

<p>¹Amplicon (bp)</p><p>²Annealing Temperature (°C)</p><p>³CTSL2, cathepsin L2; GHR, growth hormone receptor; IGF-I, insulin-like growth factor I; PI3KR1, phosphatidylinositol 3-kinase, regulatory 1.</p><p>Primer sequences used for quantitative real-time polymerase chain reaction.</p