8 research outputs found
Promoter Methylation of p16INK4A, hMLH1, and MGMT in Liquid-Based Cervical Cytology Samples Compared with Clinicopathological Findings and HPV Presence
Cervical cancer is a common cancer inflicting women worldwide. Even though, persistent infection with oncogenic Human Papillomavirus (HPV) types is considered the most important risk factor for cervical cancer development, less than 5% of women with HPV will eventually develop cervical cancer supporting that other molecular events, like methylation-dependent inactivation of tumor suppressor genes, may cocontribute in cervical carcinogenesis. We analyzed promoter methylation of three candidate genes (p16, MGMT, and hMLH1) in 403 liquid-based cytology samples. Methylation was commonly identified in both benign and pathologic samples and correlated with higher lesion grade determined by cytological, colposcopical, or histological findings, with HPV DNA and mRNA positivity of specific HPV types and p16INK4A protein expression. Overall accuracy of methylation is much lower than traditional diagnostic tests ranking it as an ancillary technique with more data needed to identify the exact value of methylation status in cervical carcinogenesis
Promoter Methylation of p16 INK4A , hMLH1, and MGMT in Liquid-Based Cervical Cytology Samples Compared with Clinicopathological Findings and HPV Presence
Cervical cancer is a common cancer inflicting women worldwide. Even though, persistent infection with oncogenic Human Papillomavirus (HPV) types is considered the most important risk factor for cervical cancer development, less than 5% of women with HPV will eventually develop cervical cancer supporting that other molecular events, like methylation-dependent inactivation of tumor suppressor genes, may cocontribute in cervical carcinogenesis. We analyzed promoter methylation of three candidate genes (p16, MGMT, and hMLH1) in 403 liquid-based cytology samples. Methylation was commonly identified in both benign and pathologic samples and correlated with higher lesion grade determined by cytological, colposcopical, or histological findings, with HPV DNA and mRNA positivity of specific HPV types and p16 INK4A protein expression. Overall accuracy of methylation is much lower than traditional diagnostic tests ranking it as an ancillary technique with more data needed to identify the exact value of methylation status in cervical carcinogenesis
Differential Effects of Stent-Graft Fabrics on Arterial Stiffness in Patients Undergoing Endovascular Aneurysm Repair
Purpose: To evaluate the differential effects of endograft fabric types
on pulse wave velocity (PWV), a valid, clinically feasible marker of
arterial stiffness, in patients undergoing endovascular repair (EVAR) of
abdominal aortic aneurysms (AAAs).
Methods: As part of a registered study,118 consecutive men (mean age
7168 years) with AAA undergoing elective EVAR were divided into groups
according to the type of fabric in the implanted endografts: 46 had
polytetrafluoroethylene (PTFE)-covered stent-grafts and 72 received
stent-grafts covered with polyester fabric. Patients with Marfan
syndrome, collagen-related disorders, and end-stage renal failure were
excluded. PWV, clinical characteristics (comorbidities, body mass index,
blood pressure, lipids, and glucose profile), novel biomarkers
[interleukin (IL)-6, IL-8, IL-10, and osteoprotegerin OPG)] were
determined at baseline and prospectively after 12 months.
Results: At baseline, standard multiple regression analysis revealed the
independent association of mean blood pressure, OPG, and AAA diameter
with PWV after adjustment for age (R-2=0.729, p=0.036). At follow-up,
serum levels of both IL-8 and IL-10 were significantly increased, while
OPG decreased in both groups (p<0.05). However, between-group comparison
revealed a more adverse effect of polyester covered endografts on serum
IL8 (p=0.001) and OPG (p=0.048) levels. At the same time, PWV was
considerably increased in both polyester (+4.12 +/- 60.33 m/s, p<0.001)
and PTFE (+2.82 +/- 0.25 m/s, p=0.003) groups; the effect was more
pronounced in the former group (p=0.033). In multivariate analysis, the
polyester endograft type emerged as an independent determinant of PWV
change after EVAR (R-2=0.460, p=0.040).
Conclusion: Both endograft types increased PWV, while recipients of
polyester covered stent-grafts showed greater PWV elevation. Further
investigation will clarify the inflammatory response and the
differential clinical impact of endograft types
The relationship of novel adipokines, RBP4 and omentin-1, with carotid atherosclerosis severity and vulnerability
Objective: We investigated the relationship of circulating novel
adipokines, retinol-binding protein 4 (RBP4) and omentin-1, with
advanced carotid atherosclerosis and ultrasound indexes of severity
(total plaque area-TPA) and plaque echogenicity and vulnerability
(Gray-Scale median - GSM score).
Methods: We enrolled 225 patients with high-grade carotid stenosis
(HGCS) who underwent carotid revascularization (73 Symptomatic patients,
152 asymptomatic patients) and 75 age-and sex-matched, asymptomatic
individuals with low-grade (< 50%) carotid stenosis (LGCS).
Seventy-three individuals without current manifestations of
atherosclerotic disease served as control group (COG). All participants
underwent carotid ultrasound with TPA and GSM score assessment.
Moreover, clinical parameters, metabolic profile, and circulating levels
of hsCRP and adipokines were assessed.
Results: RBP4 was significantly elevated in HGCS (51.44 +/- 16.23 mg/L)
compared to LGCS (38.39 +/- 8.85 mg/L), independent of symptoms
existence, whereas RBP4 levels in COG were even lower (25.74 +/- 10.72
mg/L, p < 0.001 compared to either HGCS or LGCS). Inversely, serum
omentin-1 levels were significantly lower across HGCS (490.41 +/- 172
ng/ml) and LGCS (603.20 +/- 202.43 ng/ml) than COG (815.3 +/- 185.32, p
< 0.001). Moreover, the considerable difference between HGCS and LGCS (p
< 0.001) was exclusively attributed to the excessive suppression of
omentin-1 concentrations in symptomatic versus asymptomatic (p = 0.004)
patients. HGCS and LGCS did not differ in the rest of clinical and
biochemical parameters. In multiple regression analysis, RBP4 (beta =
0.232, p = 0.025) and hsCRP (beta = 0.300, p = 0.004) emerged as
independent determinants of TPA in patients with carotid
atherosclerosis. Low serum levels of omentin-1 correlated with GSM score
and symptoms but that association was lost in multivariate analysis.
Conclusion: RBP4 serum levels were significantly elevated in patients
with established carotid atherosclerosis and were positively associated
with atherosclerosis severity. The association of low serum omentin-1
with carotid plaque echolucency requires further investigation. (C) 2014
Elsevier Ireland Ltd. All rights reserved