Lagochilascariasis leading to severe involvement of ocular globes, ears and meninges

A case report of a 31 year-old woman from Paraíba State (North-Eastern Brazil) that presented severe involvement of ocular globes, ears and meninges. Diagnosis was established after enucleation of her left eye, when adult worms were seen in the midst of a granulomatous inflammatory process. Her response to the initial treatment with levamisole and cambendazole was good, but there was a relapse after the fifth month of treatment even with maintenance doses of both medications. She later received ivermectin and albendazol and responded well.Paciente do sexo feminino, com 31 anos, procedente da Paraíba, apresentava envolvimento severo de ambos os globos oculares, com perda da visão à esquerda, comprometimento da audição e das meninges. Após enucleação do olho esquerdo fragmento de helminto identificado como Lagochilascaris minor foi observado em processo inflamatório granulomatoso. Inicialmente a paciente foi tratada com levamisol e cambendazol, com bom resultado. Verificou-se, todavia, piora do quadro após cinco meses, com eliminação de larvas do ascarídeo em lesão presente na órbita esquerda, embora a medicação fosse mantida com administração periódica. Houve boa resposta terapêutica, com regressão do quadro, após substituição dos anti-helmínticos anteriores pela associação ivermectina e albendazol

Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy

Morphostructural characterization of soil conventionally tilled with mechanized and animal traction with and without cover crop

The structural stability and restructuring ability of a soil are related to the methods of crop management and soil preparation. A recommended strategy to reduce the effects of soil preparation is to use crop rotation and cover crops that help conserve and restore the soil structure. The aim of this study was to evaluate and quantify the homogeneous morphological units in soil under conventional mechanized tillage and animal traction, as well as to assess the effect on the soil structure of intercropping with jack bean (Canavalia ensiformis L.). Profiles were analyzed in April of 2006, in five counties in the Southern-Central region of Paraná State (Brazil), on family farms producing maize (Zea mays L.), sometimes intercropped with jack bean. The current structures in the crop profile were analyzed using Geographic Information Systems (GIS) and subsequently principal component analysis (PCA) to generate statistics. Morphostructural soil analysis showed a predominance of compact units in areas of high-intensity cultivation under mechanized traction. The cover crop did not improve the structure of the soil with low porosity and compact units that hamper the root system growth. In areas exposed to animal traction, a predominance of cracked units was observed, where roots grew around the clods and along the gaps between them

Influência dos níveis de concentrado sobre os cortes comerciais, os constituintes não-carcaça e os componentes da perna de cordeiros confinados Effect of concentrate levels on retail cuts, non-carcass and leg components of crossbred feedlot lambs

Um experimento foi realizado com o objetivo de determinar a influência dos níveis de concentrado sobre o peso e o rendimento dos cortes e dos constituintes não-carcaça e a composição tecidual da perna de cordeiros mestiços de Dorper terminados em confinamento ou mestiços de Dorper x Santa Inês. Foram utilizados 32 cordeiros (não-castrados) ½ Dorper x ½ Santa Inês (peso médio inicial de 17,1 kg) alimentados com dietas contendo 30, 45, 60 e 75% de concentrado. Os cordeiros foram distribuídos em um delineamento inteiramente casualizado com quatro tratamentos e oito repetições. Os animais foram mantidos em confinamento durante 43 dias. Após o abate, as carcaças foram resfriadas por 24 horas. Foram registrados os pesos e as porcentagens dos cortes da meia-carcaça e as porcentagens de osso, músculos e tecido adiposo, avaliadas por meio de dissecação da perna. Houve efeito significativo linear crescente dos níveis de concentrado sobre os pesos de pescoço, paleta, costilhar, lombo e perna. Verificou-se também efeito significativo linear decrescente sobre os rendimentos dos cortes comerciais (pescoço, perna e paleta), excetuando-se o de costilhar, que cresceu linearmente. A espessura de gordura e a área de olho-de-lombo foram influenciadas pelos níveis de concentrado. Observou-se efeito significativo sobre os constituintes não-carcaça, exceto o trato digestório, que não foi afetado pelos níveis de concentrado. O aumento do nível de concentrado influenciou o peso e o rendimento dos cortes e órgãos internos e aumentou a proporção e a deposição de gordura na carcaça.<br>Thirty-two intact lambs averaging 17.1 kg of initial body weight were assigned to one of the following four treatments: 30, 45, 60 or 75% of dietary concentrate in a completely randomized design (eight lambs/treatment). Lambs were feedlot fed during the experimental period (43 days). After slaughter, carcasses were refrigerated for 24 h. It was measured the weights and percentages of five cuts from the half carcass as well as the proportion of muscle, bone and fat tissue from the leg. The weights of neck, shoulder, ribs, loin and leg all increased linearly by increasing the level of concentrate in the diet while the opposite was observed for the yields of neck, leg, and shoulder; yield of ribs followed the increment of concentrate in the diet and increased linearly. Fat thickness and loin eye area were both affected by the level of concentrate in the diet. The same was observed for the non-carcass components with the exception of digestive tract. Feeding increasing dietary levels of concentrate to lambs affected the weight and yield of retail cuts and internal organs and increased the proportion and deposition of fat in the carcass