Effect of vaginal pH on efficacy of dinoprostone gel for labour induction

Background: Induction of labour is defined as an intervention designed to artificially initiate uterine contractions leading to progressive dilatation and effacement of the cervix and birth of the baby. The aim of the study was to evaluate the influence of vaginal pH on the efficacy of dinoprostone gel for labor induction.Methods: A prospective study conducted on 200 subjects within 1 year in India. The Bishop score and vaginal pH (with pH paper, Sigma Chemical Company, St. Louis, USA) of subjects undergoing induction of labor with dinoprostone gel was assessed prior induction. After 6 hours of induction, PGE2 gel was repeated or labor was augmented. The vaginal pH measured was compared with age, parity, Bishop Score, time to enter into active phase of labor, and the mode of delivery. The significance of association was calculated by Chi-square test.Results: Majority subjects had pH in the range 5-6.Subjects with higher parity were associated with higher vaginal ph. Higher vaginal pH was associated with a higher Bishop score prior to induction, responded to single induction, and had a higher  number of vaginal deliveries than those with lower vaginal pH. There was no significant association found in vaginal pH and the time taken to enter into active phase of labor. Subjects with pregnancy induced hypertension were found to have higher vaginal ph.Conclusions: Parity influences vaginal pH and vaginal pH itself has a significant effect on the Bishop Score prior induction. Hence knowing the vaginal pH prior induction could be a useful tool to assess the labor outcome in induction with PGE2

Anaerobic Digestion of Olive Mill Wastewater in the Presence of Biochar

Biological treatments focused on stabilizing and detoxifying olive mill wastewater facilitate agronomic reuse for irrigation and fertilization. Anaerobic digestion is particularly attractive in view of energy recovery, but is severely hampered by the microbial toxicity of olive mill wastewater. In this work, the addition of biochar to the digestion mixture was studied to improve the stability and efficiency of the anaerobic process. Kinetics and yields of biogas production were evaluated in batch digestion tests with biochar concentrations ranging from 0 to 45 g L−1. The addition of biochar reduced sensibly the lag phase for methanogenesis and increased the maximum rate of biogas generation. Final yields of hydrogen and methane were not affected. Upon addition of biochar, soluble COD removal increased from 66% up to 84%, and phenolics removal increased from 50% up to 95%. Digestate phytotoxicity, as measured by seed germination tests, was reduced compared to raw wastewater. Addition of biochar further reduced phytotoxicity and, furthermore, a stimulatory effect was observed for a twenty-fold dilution. In conclusion, biochar addition enhances the anaerobic digestion of olive mill wastewaters by effectively reducing methanogenesis inhibition and digestate phytotoxicity, thus improving energy and biomass recovery

Computational and synthetic approaches for the discovery of HIV-1 integrase inhibitors

n/

Assessment of Curcuma longa linn. on learning and memory in rats

Background: There is an increase in elderly population and memory-related disorders. Curcuma longa linn. can induce cognitive improvement due to its antioxidant effects. This study evaluated the effect of chronic administration of C. longa linn. on learning and memory in rats.Methods: Twenty four female Albino Wistar rats were randomized into control, standard, and test groups. The control group received 0.5% methyl cellulose; the standard group received piracetam and test group received C. longa orally for 24 days. Water maze and step-through passive avoidance test were used to evaluate the effect of C. longa linn. on learning and memory. Water maze training was done from day 23 to day 26. Assessment of spatial memory was done on day 27 and retention memory on day 29.Results: The mean escape latency (EL) in control, standard, and test group was 42.48, 30.45, and 30.88 sec on day 23 which decreased to 11.08, 11.36, and 9.66 sec on day 26, respectively. The EL in all the three groups decreased from day 23 to day 26 (p<0.01). The time spent in the target quadrant was more in the test group (21.25 sec) compared to control (19.73 sec) and standard group (19.79 sec) (p=0.768). In passive avoidance test, test group showed a trend toward improvement in retention memory (p=0.293).Conclusion: C. longa linn. may be useful in enhancing learning. Further dose ranging preclinical studies are required to evaluate the efficacy of curcumin on memory

Some results on thermal stress of layered plates and shells by using Unified Formulation

This work presents some results on two-dimensional modelling of thermal stress problems in multilayered structures. The governing equations are written by referring to the Unified Formulation (UF) introduced by the first author. These equations are obtained in a compact form, that doesn&#039;t depend on the order of expansion of variables in the thickness direction or the variable description (layer-wise models and equivalent single layers models). Classical and refined theories based on the Principle of Virtual Displacements (PVD) and advanced mixed theories based on the Reissner Mixed Variational Theorem (RMVT) are both considered. As a result, a large variety of theories are derived and compared. The temperature profile along the thickness of the plate/shell is calculated by solving the Fourier&#039;s heat conduction equation. Alternatively, thermo-mechanical coupling problems can be considered, in which the thermal variation is influenced by mechanical loading. Exact closed-form solutions are provided for plates and shells, but also the applications of the Ritz method and the Finite Element Method (FEM) are presented

Synthesis, biological activity, pharmacokinetic properties and molecular modelling studies of novel 1H,3H-oxazolo[3,4-a]benzimidazoles: non-nucleoside HIV-1 reverse transcriptase inhibitors

New 1H,3H-oxazolo[3,4-a]benzimidazoles (OBZs) were synthesized as HIV-1 non-nucleoside reverse transcriptase inhibitors (NNRTI) to extend the structure-activity relationships observed for an early series of related 1H,3H-thiazolo[3,4-a]benzimidazole derivatives (TBZs). The new compounds showed inhibitory activity against the replication of various HIV-1 strains, including NNRTI-resistant strains. Testing of a representative OBZ derivative in an HPLC assay on biological fluids, indicated that the sulphur substitution appreciably improved the metabolic stability of the TBZ compound. In addition, molecular modelling studies demonstrated that OBZs, TBZs and other NNRTIs have similar structural properties, that is a butterfly-like conformation, which is a key structural requirement for reverse transcriptase inhibition

Biochar Enhances Anaerobic Digestion of Olive Mill Wastewater

The anaerobic digestion of olive mill wastewater is severely hindered by the microbial toxicity of the effluent. Successful biodegradation is usually attained only by dilution and mixing with other organic wastes. In this work the addition of biochar has been investigated as an alternative to co-digestion. In batch tests, raw olive mill wastewater selectively inhibited methanogenic bacteria even at 1:10 dilution. Acidogenesis was not affected and further biodegradation was hindered by acidification and high hydrogen partial pressure. Methanogenesis resumed after 20 days and ended after 40 days from inoculation. Overall soluble COD removal was 32.9% with a methane yield of 2.35 ± 0.27 L STP/L of digestion medium. Treatment of wastewater with 15% (w/v) poplar biochar removed 71% of inhibitory polyphenolic compounds and effectively accelerated the onset of methanogenesis, with a reduced duration of 26 days. Biochar treatment of olive mill wastewater followed by anaerobic digestion led to a higher soluble COD removal (61.6%) with a slightly decreased biogas yield of 1.62 ± 0.30 L STP/L of digestion medium

Retinal vascular tortuosity in schizophrenia and bipolar disorder

\u3cp\u3eThe micro-vasculature of retina and brain share common morphological, physiological, and pathological properties. Retina being easily accessible, retinal vascular examination provides an indirect assessment of cerebral vasculature. Considering the high prevalence of vascular morbidity in SCZ and BD a few studies have examined retinal vascular caliber and have reported increased retinal venular caliber in schizophrenia (SCZ). Retinal vascular tortuosity could serve as a better structural measure than caliber as it is static and less susceptible to pulse period variations. However, to date, no study has examined retinal vascular tortuosity in SCZ and bipolar disorder (BD). Hence, we examined retinal vascular tortuosity in comparison with healthy volunteers (HV). We included 255 subjects (78 HV, 79 SCZ, and 86 BD) in the age range of 18 to 50 years. Trained personnel acquired images using a non-mydriatic fundus camera. To measure the average retinal arteriolar tortuosity index (RATI) and retinal venular tortuosity index (RVTI), we used a previously validated, semi-automatic algorithm. The results showed significant differences across the three groups in RATI but not in RVTI; both BD and SCZ had significantly increased RATI compared to HV. There was also a significant difference between SCZ and BD, with BD having higher RATI. If shown to be of predictive utility in future longitudinal studies, it has the potential to identify patients at risk of development of adverse vascular events. As retinal vascular imaging is non-invasive and inexpensive, it could serve as a proxy marker and window to cerebral vasculature.\u3c/p\u3

Effect of Recombinant Cytokines on the Expression of Natural Killer Cell Receptors from Patients with TB or/and HIV Infection

BACKGROUND: NK cells express several specialized receptors through which they recognize and discriminate virally-infected/tumor cells efficiently from healthy cells and kill them. This ability to lyse is regulated by an array of inhibitory or activating receptors. The present study investigated the frequency of various NK receptors expressed by NK cell subsets from HIV-infected TB patients. The effect of IL-15+IL-12 stimulation on the expression of NK receptors was also studied. METHODOLOGY/PRINCIPAL FINDINGS: The study included 15 individuals each from normal healthy subjects, pulmonary tuberculosis patients, HIV-infected individuals and patients with HIV and tuberculosis co-infection. The expression of NK cell receptors was analyzed on two NK cell subsets within the peripheral blood: CD16+CD3- and CD56+CD3- using flow cytometry. The expression of inhibitory receptors (CD158a, CD158b, KIRp70, CD85j and NKG2A) on NK subsets was increased in HIV, when compared to NHS. But the response in HIV-TB was not uniform. Stimulation with IL-15+IL-12 dropped (p<0.05) the expression of CD85j and NKG2A in HIV. The basal expression of natural cytotoxicity receptors (NKp30 and NKp46) on NK cell subsets was lowered (p<0.05) in HIV and HIV-TB as compared to NHS. However, the expression of NKp44 and NKG2D was elevated in HIV. Enhanced NKp46 and NKG2D expression was observed in HIV with IL-15+IL-12 stimulation. The coreceptor NKp80 was found to be expressed in higher numbers on NK subsets from HIV compared to NHS, which elevated with IL-15+IL-12 stimulation. The expression of NK receptors and response to stimulation was primarily on CD56+CD3- subset. CONCLUSIONS/SIGNIFICANCE: IL-15+IL-12 has an immunomodulatory effect on NK cell subsets from HIV-infected individuals viz down-regulation of iNKRs, elevation of activatory receptors NKp46 and NKG2D, and induction of coreceptor NKp80. IL-15+IL-12 is not likely to be of value when co-infected with TB probably due to the influence of tuberculosis

Hybridizing the electromagnetism-like algorithm with descent search for solving engineering design problems

In this paper, we present a new stochastic hybrid technique for constrained global optimization. It is a combination of the electromagnetism-like (EM) mechanism with a random local search, which is a derivative-free procedure with high ability of producing a descent direction. Since the original EM algorithm is specifically designed for solving bound constrained problems, the approach herein adopted for handling the inequality constraints of the problem relies on selective conditions that impose a sufficient reduction either in the constraints violation or in the objective function value, when comparing two points at a time. The hybrid EM method is tested on a set of benchmark engineering design problems and the numerical results demonstrate the effectiveness of the proposed approach. A comparison with results from other stochastic methods is also included