Human CD34+ CD133+ Hematopoietic Stem Cells Cultured with Growth Factors Including Angptl5 Efficiently Engraft Adult NOD-SCID Il2rγ−/− (NSG) Mice

Increasing demand for human hematopoietic stem cells (HSCs) in clinical and research applications necessitates expansion of HSCs in vitro. Before these cells can be used they must be carefully evaluated to assess their stem cell activity. Here, we expanded cord blood CD34+ CD133+ cells in a defined medium containing angiopoietin like 5 and insulin-like growth factor binding protein 2 and evaluated the cells for stem cell activity in NOD-SCID Il2rg−/− (NSG) mice by multi-lineage engraftment, long term reconstitution, limiting dilution and serial reconstitution. The phenotype of expanded cells was characterized by flow cytometry during the course of expansion and following engraftment in mice. We show that the SCID repopulating activity resides in the CD34+ CD133+ fraction of expanded cells and that CD34+ CD133+ cell number correlates with SCID repopulating activity before and after culture. The expanded cells mediate long-term hematopoiesis and serial reconstitution in NSG mice. Furthermore, they efficiently reconstitute not only neonate but also adult NSG recipients, generating human blood cell populations similar to those reported in mice reconstituted with uncultured human HSCs. These findings suggest an expansion of long term HSCs in our culture and show that expression of CD34 and CD133 serves as a marker for HSC activity in human cord blood cell cultures. The ability to expand human HSCs in vitro should facilitate clinical use of HSCs and large-scale construction of humanized mice from the same donor for research applications.Singapore-MIT Alliance for Research and Technology ( Infectious Diseases research grant

Epidemiological Profile among Greek CrossFit Practitioners

CrossFit (CF) is a popular and rapidly expanding training program in Greece and worldwide. However, there is a lack of scientific evidence on the risk of musculoskeletal injuries related to CF in the Greek population. A self-administered survey of 1224 Greek CF practitioners aged 18 to 59 was conducted and analyzed using the Statistical Package for Social Sciences (SPSS) software. The highest percentage of the participants (34%) practiced 5 days per week for 60 min (42.2%) and had 2 days per week of rest (41.7%). A total of 273 individuals (23%) participated in CF competitions and 948 (77%) did not. The results showed that the most common injuries were muscle injuries (51.3%), followed by tendinopathies (49.6%) and joint injuries (26.6%). The shoulders (56.6%; n = 303), knees (31.8%; n = 170), and lumbar spine (33.1%; n = 177) were the most commonly injured locations. The logistic regression model showed that participation in competitions (p = 0.001), rest per week (p = 0.01), duration of training per session (p = 0.001), and frequency of training per week (p = 0.03) were statistically significant factors for injury. Training level was not a statistically significant factor for injury (p = 0.43). As CF continues to gain popularity on a global scale and the number of athletes gradually increases, it is important to monitor the safety of practitioners. Clinicians should consider participation in competitions, rest, training duration, and frequency in order to make CF safer

nandb—number and brightness in R with a novel automatic detrending algorithm

An R package for performing number and brightness image analysis, with the implementation of a novel automatic detrending algorithm

Effect of alkali promoters (K) on nitrous oxide abatement over Ir/Al2O3 catalysts

Summarization: The promoting impact of potassium (0–1 wt% K) on nitrous oxide (N2O) catalytic decomposition over Ir/Al2O3 is investigated under both oxygen deficient and oxygen excess conditions. All samples were characterized by means of X-ray powder diffraction (XRD), temperature-programmed reduction (H2-TPR), ammonia desorption (NH3-TPD) and Fourier Transform Infrared Spectroscopy of pyridine adsorption (FTIR-Pyridine). The results reveal that the K-free Ir/Al2O3 catalyst consists mainly of the IrO2 phase, exhibiting also significant Lewis acidity, which is gradually eliminated by the addition of K. Catalytic performance results showed that the deN2O performance in the absence of O2 in the feed mixture is negatively affected upon increasing potassium loading. However, under oxygen excess conditions, a pronounced effect of K is observed. Although the catalytic performance of the un-doped catalyst is drastically hindered by the presence of O2, the K-promotion notably prohibits the oxygen poisoning. The optimum deN2O performance under oxygen excess conditions is obtained with potassium loading of 0.5 wt% K, which offers complete conversion of N2O at 580 °C, instead of the corresponding 50 % N2O conversion achieved with the un-modified sample. On the basis of characterization results, it was concluded that alkali-doping in combination with oxygen excess conditions are required towards the formation of active Ir entities.Presented on: Topics in Catalysi

A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Little is known about the intermolecular dynamics and stoichiometry of the interactions of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein with its receptors and co-receptors on the host cell surface. Here we analyze timeresolved HIV-1 Env interactions with T-cell surface glycoprotein CD4 (CD4) and C-C chemokine receptor type 5 (CCR5) or C-X-C chemokine receptor type 4 (CXCR4) on the surface of cells, by combining multicolor super-resolution localization microscopy (direct stochastic optical reconstruction microscopy) with fluorescence fluctuation spectroscopy imaging. Utilizing the primary isolate JR-FL and laboratory HXB2 strains, we reveal the time-resolved stoichiometry of CD4 and CCR5 or CXCR4 in the prefusion complex with HIV-1 Env. The HIV-1 Env pre-fusion dynamics for both R5- and X4-tropic strains consists of a three-step mechanism, which seems to differ in stoichiometry. Analyses with the monoclonal HIV-1-neutralizing antibody b12 indicate that the mechanism of inhibition differs between JR-FL and HXB2 Env. The molecular insights obtained here identify assemblies of HIV-1 Env with receptors and co-receptors as potential novel targets for inhibitor design