Mycorrhizal trait data and niche characteristics for vascular plants in the Dutch flora

File includes updated mycorrhizal status, type and flexibility data with new additional references, and realized niche optima, widths and volumes along soil fertility, pH, moisture, salinity, light and temperature axes for the vascular plant species in the Dutch flora. Also, a legend is provided as a separate sheet in the file. More details can be found in the README file

Variation in the AMF communities present in soil at Järvselja forest reserve.

<p>The results of PERMANOVA analysis are shown. The seasonal model describes variation between sampling month (May, June, July and September) and sample location (SampleID) in Plot A; the spatial model describes variation between Plots A, B and C in September. In the seasonal model, the significance of explanatory variables was not sensitive to their order in the model.</p

Change in soil AMF community similarity as a function of spatial and temporal distance.

<p>A) spatial - soil samples from 10×10 m plots in September and; B) temporal - soil samples from plot A in May, June, July and September. The data points show pairwise similarity estimates between all samples; the bold red line shows the relationship between similarity and distance in the real data; the faint black lines show the same relationship in 1000 randomised data matrices. Note that the x axis does not represent direction in space or time; thus greater values denote the greatest distance between samples – e.g. between May and September – and not the timing or location of samples <i>per se</i>. The slope of the real model was steeper than the randomised set for the spatial model (P<0.01) but not the temporal model (P = 0.57).</p

Rarefaction analysis of soil AMF samples from Järvselja forest reserve.

<p>Mean VT richness is estimated in relation to the number of reads analysed for subsets of the data representing soil samples collected in different months and from different plots.</p

Sampling design used to study soil AMF communities in Järvselja forest reserve.

<p>Nine soil samples (SampleID 1–9) were collected in each of three 10×10 m plots (A, B and C). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041938#pone-0041938-t001" target="_blank">Table 1</a> for further details of the sampling design.</p

Figure 3. Two-dimensional non-metric multi-dimensional scaling (NMDS) plots of variation in soil AMF community composition.

<p>A) in three spatially distinct 10×10 m plots (A–C) in September; and B) in plot A in May, June, July and September.</p

Appendix A. Description of the plant traits used in the analysis.

Description of the plant traits used in the analysis

Appendix C. PCA biplot of plant trait and species occurrence data from the total MycoFlor data set.

PCA biplot of plant trait and species occurrence data from the total MycoFlor data set

The abundance of soil microbes (A) and nutrients (B) in May (open bars) and July (closed bars).

<p>The abundance of arbuscular mycorrhizal (AM) fungi, other fungi and bacteria was estimated as the content of respective ester-linked fatty acid (y-axis on graph A, see Methods for details). Soil N concentration was measured in % (left y-axis of graph B) whereas P and K content were measured as mg/kg (right y-axis of graph B). Significant differences (Linear Mixed-Effect Models; p<0.05) in the measured parameters over time are marked with *.</p

Appendix B. Details of phylogenetic correction methods.

Details of phylogenetic correction methods