Trendy nr 4/2016

W nowym numerze TRENDÓW piszemy o kompetencjach kluczowych – informatycznych, matematycznych, technicznych, umiejętności uczenia się, innowacyjności i przedsiębiorczości. W kwietniu 2017 r. zastanowimy się nad tym, jak kształtować pozostałe – umiejętność porozumiewania się w języku ojczystymi obcym, kompetencje społeczne i obywatelskie, świadomość i ekspresję kulturalną. Kompetencje kluczowe to kompetencje, które wspierają rozwój osobisty, włączanie w życie społeczne, aktywne obywatelstwoi możliwość znalezienia zatrudnienia. Proces kształtowania i rozwijaniaw sobie kompetencji kluczowych trwa przez całe życie i nigdy się niekończy. Autorzy artykułów w tym numerze udowadniają, że kształtowanie kompetencji kluczowych nie jest trudne – to codzienność i stały element pracy w szkołach

Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism

The MSC16 cucumber (Cucumis sativus L.) mitochondrial mutant was used to study the effect of mitochondrial dysfunction and disturbed subcellular redox state on leaf day/night carbon and nitrogen metabolism. We have shown that the mitochondrial dysfunction in MSC16 plants had no effect on photosynthetic CO2 assimilation, but the concentration of soluble carbohydrates and starch was higher in leaves of MSC16 plants. Impaired mitochondrial respiratory chain activity was associated with the perturbation of mitochondrial TCA cycle manifested, e.g., by lowered decarboxylation rate. Mitochondrial dysfunction in MSC16 plants had different influence on leaf cell metabolism under dark or light conditions. In the dark, when the main mitochondrial function is the energy production, the altered activity of TCA cycle in mutated plants was connected with the accumulation of pyruvate and TCA cycle intermediates (citrate and 2-OG). In the light, when TCA activity is needed for synthesis of carbon skeletons required as the acceptors for NH4+ assimilation, the concentration of pyruvate and TCA intermediates was tightly coupled with nitrate metabolism. Enhanced incorporation of ammonium group into amino acids structures in mutated plants has resulted in decreased concentration of organic acids and accumulation of Glu

Supplementary NTA figure.

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Supplemental western blot figure.

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Western blot analysis of protein lysates from isolated from canine plasma.

Western blot analysis of protein lysates from isolated from canine plasma.</p

NanoSight analysis of particle size distribution of EVs isolated from canine plasma.

(A) Line graph of healthy control, DLBCL and adenocarcinoma patient particle size distribution. (B) Bar graph of total EV concentration of particles / ml and (C) modal size of healthy control, DLBCL and adenocarcinoma patient EV samples.</p

Haematological and chosen biochemical parameter assessment of the antioxidant system in red deer (Cervus elaphus) blood in early and late pregnancy

The aim of this study was to investigate changes in haematological parameters and the antioxidant system in the early and late pregnancy of red deer (Cervus elaphus)

Differential Regulation of Cystic Fibrosis Transmembrane Conductance Regulator by Interferon gamma in Mast Cells and Epithelial Cells

Peer reviewed: YesNRC publication: N

Machine learning system for automated blood smear analysis

In this paper the authors propose a decision support system for automatic blood smear analysis based onmicroscopic images. The images are pre-processed in order to remove irrelevant elements and to enhancethe most important ones – the healthy blood cells (erythrocytes) and the pathologic ones (echinocytes). The separated blood cells are analysed in terms of their most important features by the eigenfaces method. The features are the basis for designing the neural network classifier, learned to distinguish between erythrocytes and echinocytes. As the result, the proposed system is able to analyse the smear blood images in a fully automatic way and to deliver information on the number and statistics of the red blood cells, both healthy and pathologic. The system was examined in two case studies, involving the canine and human blood, and then consulted with the experienced medicine specialists. The accuracy of classification of red blood cells into erythrocytes and echinocytes reaches 96%