22 research outputs found
LipoproteinâAssociated Phospholipase A2 Activity Is a Marker of Risk But Not a Useful Target for Treatment in Patients With Stable Coronary Heart Disease
Background: We evaluated lipoproteinâassociated phospholipase A2 (LpâPLA2) activity in patients with stable coronary heart disease before and during treatment with darapladib, a selective LpâPLA2 inhibitor, in relation to outcomes and the effects of darapladib in the STABILITY trial. Methods and Results: Plasma LpâPLA2 activity was determined at baseline (n=14 500); at 1 month (n=13 709); serially (n=100) at 3, 6, and 18 months; and at the end of treatment. Adjusted Cox regression models evaluated associations between LpâPLA2 activity levels and outcomes. At baseline, the median LpâPLA2 level was 172.4 ÎŒmol/min per liter (interquartile range 143.1â204.2 ÎŒmol/min per liter). Comparing the highest and lowest LpâPLA2 quartile groups, the hazard ratios were 1.50 (95% CI 1.23â1.82) for the primary composite end point (cardiovascular death, myocardial infarction, or stroke), 1.95 (95% CI 1.29â2.93) for hospitalization for heart failure, 1.42 (1.07â1.89) for cardiovascular death, and 1.37 (1.03â1.81) for myocardial infarction after adjustment for baseline characteristics, standard laboratory variables, and other prognostic biomarkers. Treatment with darapladib led to a â65% persistent reduction in median LpâPLA2 activity. There were no associations between onâtreatment LpâPLA2 activity or changes of LpâPLA2 activity and outcomes, and there were no significant interactions between baseline and onâtreatment LpâPLA2 activity or changes in LpâPLA2 activity levels and the effects of darapladib on outcomes. Conclusions: Although high LpâPLA2 activity was associated with increased risk of cardiovascular events, pharmacological lowering of LpâPLA2 activity by â65% did not significantly reduce cardiovascular events in patients with stable coronary heart disease, regardless of the baseline level or the magnitude of change of LpâPLA2 activity
Design and synthesis of steroid mimetic libraries using solid phase techniques
This thesis deals with the design and synthesis of biologically active
ligands for nuclear hormone receptors using combinatorial techniques.
In the first part, a
5,6,6a,7,8,9,10,10a-octahydrobenzo[f]quinolin-3(4H)-one tricyclic core
structure was designed mimicking the ABC-rings of a steroid. The core
structure was synthesized from 7,8-dihydroquinoline-2,5(1H,6H)-dione with
the AB-rings already in place. Two different approaches towards the
preparation of the C-ring were tested, i.e. using either a Robinson
annulation or a Diels-Alder cyclization where only the latter
accomplished the formation of the C-ring. The ligands prepared showed low
affinity for the nuclear hormone receptors AR, ER, GR, MR, PR, however,
and this core structure was therefore abandoned.
The second part consists of a study of selective N-alkylation of
2-pyridones. A solid phase method was developed where 2-halopyridines
were attached to a Wang-resin and subsequently treated with alkyl halides
to alkylate and cleave the substrate in tandem, thus generating N-alkylated-pyridones.
In the third part, a 6-phenylquinolin-2(1H)-one core structure is
mimicking the ABD-rings of a steroid. The core structure was prepared
from from 6-bromo2chloroquinoline and subsequently attached to a
Wang-resin, whereupon the Dring was added to the 6-bromo moiety of the
quinoline via a Suzuki-coupling. The phenolic position was alkylated, and
the product was cleaved from the resin using the tandem alkylation
cleavage method described earlier to generate N-alkylated
6-phenyl-quinolones. This library showed moderate to high affinity
towards nuclear hormone receptors.
In the final section, a new core structure was designed, fusing a
pyrazole-ring onto the Aring on an AB-steroid ring system to generate a
benzoindazole scaffold. Buchwald's palladium catalyzed alpha-arylation
method was initially employed to derivatize the scaffold at the
6-position, but the reaction failed when transferred to solid phase. The
6phenyl-benzoindazole core structure was then redesigned to a
benzoindazole-5hydrazone scaffold, introducing diversity at the hydrazone
moiety. Best results were obtained using a solid phase approach, and the
scope and limitations in terms of the substituents was thus investigated
Development of a Semicontinuous Spray Process for the Production of Superhydrophobic Coatings from Supercritical Carbon Dioxide Solutions
Superhydrophobic surfaces have been fabricated in a continuous spray process, where an alkyl ketene dimer (AKD) wax is dissolved in supercritical carbon dioxide (scCO(2)) and sprayed onto the substrate. The mass of extracted AKD from scCO2 has been investigated as well as the pressure, temperature, and flow of CO2 at the steady-state spray conditions. Several different substrates such as glass, aluminum, paper, poly(ethylene terephthalate) (PET), and polytetrafluoroethylene (PTFE) have been successfully coated, and the superhydrophobic properties have been evaluated by measurement of water contact angle, water drop friction, scanning electron microscopy (SEM), and surface topography. The most efficient spray process, considering surface properties and mass of extracted AKD, is obtained at the lowest temperature investigated, 67 degrees C, and the highest pressure evaluated in this study, 25 MPa. We also show that the influence of preexpansion conditions (p, T) on the surface temperature at the selected spray distance (3 cm) is negligible by measurement with an infrared camera during spraying
Xyloglucan-Functional Latex Particles via RAFT-Mediated Emulsion Polymerization for the Biomimetic Modification of Cellulose
Herein,
we report a novel class of latex particles composed of
a hemicellulose, xyloglucan (XG), and polyÂ(methyl methacrylate) (PMMA),
specially designed to enable a biomimetic modification of cellulose.
The formation of the latex particles was achieved utilizing reversible
additionâfragmentation chain transfer (RAFT) mediated surfactant-free
emulsion polymerization employing XG as a hydrophilic macromolecular
RAFT agent (macroRAFT). In an initial step, XG was functionalized
at the reducing chain end to bear a dithioester. This XG macroRAFT
was subsequently utilized in water and chain extended with methyl
methacrylate (MMA) as hydrophobic monomer, inspired by a polymerization-induced
self-assembly (PISA) process. This yielded latex nanoparticles with
a hydrophobic PMMA core stabilized by the hydrophilic XG chains at
the corona. The molar mass of PMMA targeted was varied, resulting
in a series of stable latex particles with hydrophobic PMMA content
between 22 and 68 wt % of the total solids content (5â10%).
The XG-PMMA nanoparticles were subsequently adsorbed to a neutral
cellulose substrate (filter paper), and the modified surfaces were
analyzed by FT-IR and SEM analyses. The adsorption of the latex particles
was also investigated by quartz crystal microbalance with dissipation
monitoring (QCM-D), where the nanoparticles were adsorbed to negatively
charged model cellulose surfaces. The surfaces were analyzed by atomic
force microscopy (AFM) and contact angle (CA) measurements. QCM-D
experiments showed that more mass was adsorbed to the surfaces with
increasing molar mass of the PMMA present. AFM of the surfaces after
adsorption showed discrete particles, which were no longer present
after annealing (160 °C, 1 h) and the roughness (<i>R</i><sub>q</sub>) of the surfaces had also decreased by at least half.
Interestingly, after annealing, the surfaces did not all become more
hydrophobic, as monitored by CA measurements, indicating that the
surface roughness was an important factor to consider when evaluating
the surface properties following particle adsorption. This novel class
of latex nanoparticles provides an excellent platform for cellulose
modification via physical adsorption. The utilization of XG as the
anchoring molecule to cellulose provides a versatile methodology,
as it does not rely on electrostatic interactions for the physical
adsorption, enabling a wide range of cellulose substrates to be modified,
including neutral sources such as cotton and bacterial nanocellulose,
leading to new and advanced materials
Two-year outcomes in patients admitted with non-ST elevation acute coronary syndrome:results of the OASIS registry 1 and 2
Acute coronary syndrome continues to have significant long-term morbidity and mortality. This study sought to compare baseline characteristics, practice patterns and clinical outcomes for patients with non-ST elevation acute coronary syndrome from a broad range of low-, middle- and high-income countries
Building Custom Polysaccharides in Vitro with an Efficient, Broad-Specificity Xyloglucan Glycosynthase and a Fucosyltransferase
The current drive for applications of biomass-derived compounds, for energy and advanced materials, has led to a resurgence of interest in the manipulation of plant polymers. The xyloglucans, a family of structurally complex plant polysaccharides, have attracted significant interest due to their intrinsic high affinity for cellulose, both in muro and in technical applications. Moreover, current cell wall models are limited by the lack of detailed structureâproperty relationships of xyloglucans, due to a lack of molecules with well-defined branching patterns. Here, we have developed a new, broad-specificity âxyloglucan glycosynthaseâ, selected from active-site mutants of a bacterial endoxyloglucanase, which catalyzed the synthesis of high molar mass polysaccharides, with complex side-chain structures, from suitable glycosyl fluoride donor substrates. The product range was further extended by combination with an Arabidopsis thaliana α(1â2)-fucosyltransferase to achieve the in vitro synthesis of fucosylated xyloglucans typical of dicot primary cell walls. These enzymes thus comprise a toolkit for the controlled enzymatic synthesis of xyloglucans that are otherwise impossible to obtain from native sources. Moreover, this study demonstrates the validity of a chemo-enzymatic approach to polysaccharide synthesis, in which the simplicity and economy of glycosynthase technology is harnessed together with the exquisite specificity of glycosyltransferases to control molecular complexity