"Active" Weed Seed Bank: Soil Texture and Seed Weight as Key Factors of Burial-Depth Inhibition

The ability of weeds to survive over time is highly dependent on an ecological strategy that ensures a high level of viable seed remains in the soil. Seed bank persistence occurs because of the specific characteristics of seed dormancy and longevity and the hypoxic microenvironment, which surrounds the buried seeds. These experiments investigate the role of soil texture, burial depth, and seed weight in seed bank dynamics. Seeds of twelve weed species are sown at increasing depths in various soil textures, and emergence data are used to detect the burial depth at which 50% and 95% inhibition is induced, using appropriate regressions. Clay soil is found to increase the depth-mediated inhibition, while it is reduced by sandy particles. In each soil texture, the highest level of inhibition is found for the smallest seeds. Seed weight is found to be closely related to the maximum hypocotyl elongation measured in vitro, and consequently, the seedlings are unable to reach the soil surface beyond a certain depth threshold. However, the threshold of emergence depth is always lower than the potential hypocotyl elongation. The depth-mediated inhibition of buried seeds is even more pronounced in clay soil, highlighting that the small size of clay particles constitutes a greater obstacle during pre-emergence growth. Finally, the role of soil texture and weed seed size are discussed not only in terms of evaluating the layer of “active” seed bank (soil surface thickness capable of giving rise to germination and emergence), but also in terms of developing a consistent and persistent seed bank. Finally, the role of soil texture and weed seed size are discussed, and the layer of “active” seed bank (the soil surface thickness that enables germination and emergence) is assessed with the aim of developing a consistent and persistent seed bank. Assessing seed bank performance when buried under different soil textures can help increase the reliability of the forecast models of emergence dynamics, thus ensuring more rational and sustainable weed management

Qualitätsvergleich von ökologischen und konventionallen Hartweizen- und Weichweizenproben mittels der Biokristallisationsmethode

Die Weizenproben aus dem MASCOT- Versuch in Toscanien Italiens (Mediterranean Arable System COmparision Trial), der seit 6 Jahren durch das Zentrum für Landwirtschaftliche Forschung CIRAA (Interdepartmental Centre for Agri-environmental Research „E. Avanzi”) der Universität in Pisa geleitet wird, wurden mit der Biokristallisationsmethode ausgewertet. Die Proben stammen aus einem RCB Versuch in dreifacher Feldwiederholung. Die Kristallbilder wurden visuell und mittels des Computerprogramms "Image J“ ausgewertet

SIMBIO-VEG: an interdisciplinary project for improvement of organic systems and meth-ods in arable and vegetable cropping

SIMBIO-VEG is the first large interdisciplinary research project on organic farming ever being funded in Italy. The project partnership is composed of eight Working Units including Universities as well as public and private research centres and associa-tions, with a high degree of interaction among them. SIMBIO-VEG aims to acquire knowledge on the func-tioning of organic arable and vegetable cropping systems, as to (1) optimise systems management and the cultural practices (methods) therein, and improve the understanding of how organic systems and meth-ods influence produce quality and environmental impact. SIMBIO-VEG activities are structured in four strongly integrated research lines. Line 1 aims to understand the functioning of organically-managed agroecosystems and evaluate the technical and agro-nomic feasibility of crop production methods applied therein. Line 2 aims to evaluate the effects of organic systems and methods on overall produce quality, including both standard and innovative methods. Line 3 aims to evaluate the sustainability of organic sys-tems and methods through assessment of parameters and indicators related to soil quality, environmental impact, and economic viability. Lastly, line 4 is aimed to favour diffusion of results and innovation transfer through a comprehensive set of activities

Targeted timing of hairy vetch cover crop termination with roller crimper can eliminate glyphosate requirements in no-till sunflower

No-till cropping systems with cover crops can improve soil health, but often rely on glyphosate, which is a contentious herbicide. In this study, we investigated whether a system based on the direct sowing of sunflower (Helianthus annuus) in the dead mulch of a roller-crimped hairy vetch (Vicia villosa) could be competitive with a system where glyphosate is also sprayed to terminate the cover crop and to control weeds. We hypothesized that optimum timing of roller-crimping would be key to eliminate glyphosate requirements while maintaining sunflower performance. In a 3-year on-farm experiment, we compared three vetch termination stages (early: pre-flowering; Intermediate: beginning of flowering; late: 70% flowering) and three glyphosate rates (Nil, half and full, i.e. 1440 g of active ingredient per hectare). Vetch biomass increased progressively from early to late termination stages, and ranged between 414 and 658 g m−2. Higher vetch biomass was correlated with lower weed biomass. Treatments had inconsistent effects on weed diversity and composition, largely determined by the interactions between treatments and seasonal (different years) or local factors (different fields). Glyphosate-based treatments seemed to select for aggressive weed species, but no clear species filtering effect based on ecological or functional traits was detected. Shannon H’ was positively correlated with sunflower grain yield below a weed dry biomass threshold of 150 g m−2. Crop yield with early termination stage was a failure without glyphosate application. However, crop yield with late vetch termination was acceptable, being at par or 15% higher (mean of first and second years) in no-glyphosate compared with glyphosate-based treatments. Crop gross margins showed the same trend (+33% for no-glyphosate compared with glyphosate-based treatments). This study, for the first time, shows that targeted timing of roller-crimped hairy vetch in no-till sunflower can result in equal agronomic and economic performances as addition of glyphosate

Long term effects of cover crops on weeds in Mediterranean low input arable management systems

The introduction of cover crops (CC) in crop rotations is a key tool to control weed and ameliorate soil conditions in low input arable systems. In 1992 a long term experiment (LTE) was set up at Centre for Agri-environmental Research “E. Avanzi (CIRAA), University of Pisa to determine the combined effect of tillage intensity, nitrogen fertilization levels and CC types on soil quality, crop yield and weed communities. The LTE is based on 4-year crop rotation (maize, durum wheat, sunflower, durum wheat) with cover crops grown twice, in between wheat and summer crops. Two tillage systems were compared: i) a conventional system (CS), with annual ploughing (30 cm depth); ii) a low input system (LIS), with chiseling (30 cm depth) for summer crops and no till for wheat. In both systems cover types were: control; Brassica juncea L.;Trifolium squarrosum L.; and Vicia villosa Roth.). Four Nitrogen levels (from 0 to a maximum rate, varying across crop type) were applied. The experiment was replicated in 4 blocks for a total of 128 plots (21 x 11 m). Weed and CC above-ground biomass were assessed at CC termination, while weed species cover and total biomass were assessed at harvest. Data collected in CC (2011, 2014), sunflower (2012), durum wheat (2012) were used to calculate species richness, weed community diversity indices (Shannon’s H’ and inverse Simpson index) and Pielou equitability. Weed biomass and cover were analyzed by split-split plot ANOVA. Weed community composition was analyzed by a permutational multivariate analysis of variance based on Bray Curtis dissimilarity and Non-metric Multidimensional Scaling

Can Shading Affect Nitrogen Fixation Of Forage Legume Swards? An Assessment Of B-Value Through The 15N Natural Abundance Method

Tree-based intercropping systems are gaining pace as a land-use strategy to cope with climate change and provide environmental, economic, and social benefits. The integration of nitrogen-fixing crops between trees can be a solution to increase the land productivity and reduce the reliance on external inputs by increasing nitrogen (N) availability and then both tree and crop growth. Intercropping perennial legumes with trees can also reduce nitrogen losses, due to the higher amount of N accumulated in stable forms in the soil due to biological N₂-fixation and N root compartimentation. On the other hand tree competition for light, water and nutrients eventually could limit legume growth and N₂-fixation. The isotopic method based on ¹⁵N natural abundance is one of the most used methods to assess Biological Nitrogen Fixation (BNF). The B-value, that is defined as the δ¹⁵N value of a legume when completely dependent on N₂-fixation for satisfying its N demand, is of primary importance for BNF estimations. The B-value may vary with species, plant age at harvest and growing conditions, e.g. light availability. Therefore, the B-value found in literature could not be representative for all legumes and environments, in particular for legumes grown intercropped with trees and thus subject to shading conditions. In this pot experiment we assessed the B-value of several forage legumes, as affected by different levels of simulated shading and grown in N-free medium. A greenhouse pot experiment was established in March 2021 at the Department of Agriculture, Food and Environment (DAFE) of the University of Pisa to determine the B-value for ¹⁵N calculations about two forage legume species grown in a field trial located at the Center of Agri-Envirnomental Research “Enrico Avanzi” of the University of Pisa, San Piero a Grado (Pisa) (43°41'6.97"N 10°20'29.22"E), using the same shade treatments

Ground Beetle (Coleoptera: Carabidae) Assemblages and Slug Abundance in Agricultural Fields Under Organic and Low-Input Conventional Management Within a Long-Term Agronomic Trial in Central Italy

Inside a long-term agronomic trial aimed at evaluating the effects of organic and low-input conventional management systems on soil fertility and arable crop production, we selected six fields bordered by hedgerows, three under each management system. Here, we analyzed the carabid assemblages and the slug abundance. Samplings took place in five different periods, across 1 yr of observations. The carabid abundances were similar in organic and conventional fields. The Shannon–Wiener diversity index (H’) showed a higher value in the conventional fields, although in the organic fields, a higher number of species were observed. The multivariate analysis described similar carabid communities, but excluding the period factor, it showed a significant influence of the management system. There was no difference between the captures of traps placed along the hedgerow and in the middle, whereas in the conventional fields, the hedgerow traps captured a higher number of specimens, showing a role of the hedgerow as carabid reservoir. The slugs were present mainly while green manure was grown on the organic fields where also Poecilus cupreus Linné, 1758 (Coleoptera: Carabidae) was captured abundantly

Battery Electric Buses or Fuel Cell Electric Buses? A Decarbonization Case Study in the City of Brescia, Italy

Nowadays, designing and adopting sustainable and greener transport systems is of upmost interest. The European Commission and different EU countries are developing plans and programs—but also delivering resources—aimed at the decarbonization of cities and transport by 2030. In this paper, the case study of the city of Brescia, a city of about 200,000 inhabitants located in northern Italy, is addressed. Specifically, a preliminary operational and financial feasibility study is performed assuming the replacement of the entire compressed natural gas (CNG) powered bus fleet of a specific line; the two alternatives considered are battery electric buses (BEBs) and fuel cell electric buses (FCEBs). For the comparison and evaluation of the two alternatives, specific economic parameters of the three alternatives (BEB, FCEB and the current solution CNGB) were considered: CAPEX (CAPital EXpenditure) and OPEX (OPerational EXpenditure). This allowed us to determine the TCO (total cost of ownership) and TCRO (total cost and revenues of ownership) along three annuities (2022, 2025 and 2030). For the BEB alternative, the TCO and TCRO values are between EUR 0.58/km and EUR 0.91/km. In the case of the FCEB solution, the values of TCO and TCRO are between EUR 1.75/km and EUR 2.15/km. Considering the current CNGB solution, the TCO and TCRO values range between EUR 1.43/km and EUR 1.51/km

Rye (Secale cereale L.) and squarrose clover (Trifolium squarrosum L.) cover crops can increase their allelopathic potential for weed control when used mixed as dead mulch

Cover crops are essential tools in agro-ecosystems for reduc- ing the reliance on synthetic inputs and associated environmental risks. Alongside their benefits to soil fertility, cover crops can con- trol weeds by their competitive and allelopathic attributes. Laboratory and field experiments were conducted to assess the allelopathic potential of two cover crop species, rye (Secale cereale L.) and squarrose clover (Trifolium squarrosum L.), alone or in a mixture, on seed germination and growth of arable weeds. Aqueous extracts of the two cover crops and their mixture were tested in a bioassay on Conyza canadensis (L). Cronq., Amaranthus retroflexus L. and Digitaria sanguinalis (L.) Scop. In vitro effects of aqueous extracts varied in a dose-dependent manner, with cover crops and weed species. All three extracts were able to reduce the germination of A. retroflexus (–87%) consider- ably. Inhibitory effects by rye and mixture extracts on radicle growth of all weed species ranged between 51 and 82%. Rye extract was the best at reducing shoot length of C. canadensis and D. sanguinalis (–39 to 44%), while squarrose clover was more effective on A. retroflexus (–79%). Plant extracts also delayed the germination time of weed species with a substantial effect of the mixture on C. canadensis seeds. In the field experiment, no signif- icant weed suppression was provided by cover crop residues incorporated as green manure compared to control plots, despite tillage being more effective in reducing weed density than no-till. Still, the mulch of the mixture controlled weed emergence signif- icantly better than single cover crop mulches. The chemical char- acterization of cover crop residues, both shoots and roots, revealed a notable richness of allelopathic phenolic acids and flavonoids, which may constitute potential natural herbicides through slow decomposition. From the analysis of the aqueous extracts, other non-analysed and/or unidentified water-soluble allelopathic com- pounds should underlie the phytotoxicity observed in vitro, at least for rye. For cover crop mixture, positive interactions among plant materials leading to a better release of allelochemicals and weed- ing effectiveness are discussed according to chemical profiles and field data. Our study demonstrated the allelopathic activity of the cover crops and their potential to be included in weed manage- ment strategies according to cropping system needs. Additional trials are needed to confirm the performance of cover crop residues under field conditions

Light reduction affected agronomic performance and nutritive value of temporary grassland swards in a Mediterranean rainfed plot trial

In Italy, traditional olive orchards are characterised by low tree density (100-300 ha-1) allowing the cultivation of forage and crops under the tree canopy (Paris et al., 2019). Eichhorn et al. (2006), reported that in Central Italy there are 20000 ha of farmland identified as a silvoarable olive orchard. The intercropping of perennial legumes and trees is a key strategy to improve nutrient cycle of silvoarable systems, due to the higher amount of nitrogen (N) accumulated in stable forms in soil due by biological nitrogen-fixation (Hernandez-Esteban et al., 2019; Sanna et al., 2019), leading to a request for reduction of inorganic N fertilisation. Perennial legumes can also provide a continuous soil cover during the entire year reducing soil loss risk (Vallebona et al., 2016). In the Mediterranean basin, the most important perennial legume is alfalfa (Medicago sativa L.). Previous studies reported that alfalfa nutritive value was not negatively affected by tree presence (Mantino et al., 2021), whereas legume production was reduced due the competition for resources such as water (Nasielski et al., 2015), nutrients (Isaac et al., 2014) and light (Mantino et al., 2021). In Tuscany, sulla (Hedysarum coronarium L.) an autochthonous biennial legume is appreciated for its rusticity, productivity, and quality and it is intercropped with Italian ryegrass (Lolium multiflorum Lam.) for a better utilisation as pasture. In 2019, a rainfed field plot trial was established to evaluate agronomic performance and nutritive value of different perennial forage species grown under different levels of light reduction, aiming to start a selection of shade tolerant forage crops. In October, the plot trial was established in Pisa, on a clay-loam soil with pH of 8.1 and 2.5 % w/w of organic matter content in the topsoil (0-0.3 m). Before sowing, 100 kg ha-1 of P2O5 were applied. The experimental layout complies with a two-factor randomized complete block design with four replicates (18 m2 sizing each plot). The first factor included five different swards: i) sulla cv. Silvan, (ii) ryegrass cv. Teanna, (iii) mix of sulla cv. Silvan and ryegrass, 50:50 (iv) mix of sulla cv. Silvan, sulla cv. Chiara Stella and sulla cv. Bellante 33:33:33 and (v) alfalfa cv. Messe. The second factor included three increasing shading levels: S0) the control representing full light availability, S25) and S50), corresponding to a reduction of potential light availability of 25 and 50% respectively. As previously tested by Varella et al. (2011), shading was provided by woody slats, N-S oriented, 2.0 m long and 0.10 m wide, with a distance between each slat of 0.10 m for S50 and 0.20 m for S25, covering a total surface of 4 m2. After sowing, slats were placed at 0.8 m above ground level. Yield and nutritive value of herbage mass and N2 fixation were evaluated for two consecutive years. Herbage biomass was not affected by the reduction of the 50% of light in ryegrass and ryegrass-sulla mixture while it was negatively affected in alfalfa and sulla. Conversely, the 25% of shade level had no effect on legume yield