Profession, Professionalität, Professionalisierung

Alignment of LH receptor amino acid sequences obtained from the UniProt database ( http://www.uniprot.org ). Homo sapiens LHCGR (UniProt identifier: P22888), Mus musculus Lhr (P30730) and Rattus norvegicus Lhr (P16235) sequences were aligned by the UniProt online tool Clustal Omega 1.2.1 ( http://www.uniprot.org/align ). Boxes indicate sequence divergence; :=conservation of strong groups;. = conservation of weak groups or no consensus. (DOC 92 kb

Study Design.

<p>Patients subjected to prophylactic infliximab [IFX] 5 mg/Kg after surgery and in full remission after 3 years of therapy showed endoscopic recurrence in 83% of cases when the medication was stopped. Those with recurrence (n = 10) re-started IFX in a dose optimization study in which we showed that 3 mg/Kg were sufficient to re-induce and maintain endoscopic remission for 1 year in all patients (ref.2). Five of these ten patients participated in the current study. Antibodies To Infliximab [ATI] and Infliximab Trough Levels [ITL] were measured and compared to those of CD patients who did not undergo surgery in remission on a standard 5 mg/Kg IFX dose. After an additional 18 months the patients treated with 3 mg/Kg IFX underwent colonoscopy.</p

Infliximab concentration (A), Highly Sensitive [HS]–CRP (B) and Fecal Calprotectin (C) during infliximab therapy.

<p>Empty long dash lines: control patients (treated with infliximab 5 mg/Kg); Filled lines: study subjects (treated with infliximab 3 mg/Kg). Square dot lines indicate the upper limit of the normal range for HS-CRP and Fecal Calprotectin. Values are reported as mean and standard error (vertical bars). The asterisk denotes a significant difference (p < 0.05) between controls and study subjects at a given time point during the 8-week therapeutic interval.</p

Additional file 2: of Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro

Comparison between LH- and hCG-induced production of 17alpha-OH-progesterone measured by LC/MS. In order to confirm the reliability of testosterone levels detected by immunoassay (Fig. 4), the hormone was measured in 24 h-stimulated cells by EC80 LH and hCG, in the presence of 500 μM IBMX. Total steroid levels were measured by LC/MS and represented as means ± SEM (ng/ml; n = 2). (TIF 351 kb

Additional file 3: of Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro

Androstenedione levels measured by LC/MS, in LH- or hCG-stimulated Leydig cells. Androstenedione was measured in 24 h-stimulated cells by EC80 LH and hCG, in the presence of 500 μM IBMX. Hormone levels were means ± SEM (ng/ml; n = 2). (TIF 372 kb