Building Information Modeling as an Effective Process for the Sustainable Re-Shaping of the Built Environment

This paper focuses on the definition of a method supported by digital processes for a sustainable and user-orientated re-design of the existing building stock. Based on the analysis of the methodological and procedural aspects of the computational approach to architectural design in relation to different performance conditions, the research addresses the adoption of Building Information Modeling (BIM), intended as a powerful method for coordinating the complexity of the multiple, interdisciplinary and conflicting aspects involved in the rehabilitation of buildings. In addition to the advantages in terms of control and management, the BIM process has proven its effectiveness in tackling the issue of sustainability, allowing all actors involved in the research to share information and pro-actively control various outcomes of a building’s performance, such as energy and environmental quality. To show the opportunities and limitations of the digital management in information-based processes, the activities carried out in the framework of the European Horizon 2020 project “Pro-GET-onE—Proactive synergy of inteGrated Efficient Technologies on buildings’ Envelopes” are reported. The research, based on a case study method, which is applied to a student residence in Athens, demonstrates that BIM possesses great potentialities for developing effective and efficient construction and renovation processes toward buildings with high quality standards

Development and validation of a rapid LC-MS/MS method for the detection of 182 novel psychoactive substances in whole blood

INTRODUCTION: The analysis of novel psychoactive substances (NPS) represents a challenge in forensic toxicology, due to the high number of compounds characterized by different structures and physicochemical properties both among different subclasses and within a single subclass of NPS. The aim of the present work is the development and validation of a targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection of NPS in whole blood.MATERIALS AND METHODS: A protein-precipitation based LC-MS/MS method for the detection of more than 180 NPS was developed and validated by assessing the following parameters: selectivity, linearity, accuracy, precision, limit of detection (LOD) and of quantification (LOQ) recovery, and matrix effect. Then, the method was applied to real forensic samples.RESULTS: The method allowed the identification of 132 synthetic cannabinoids, 22 synthetic opioids, and 28 substances among synthetic cathinones, stimulants, and other drugs. Validation was successfully achieved for most of the compounds. Linearity was in the range of 0.25-10ng/ml for synthetic cannabinoids and 0.25-25ng/ml for other drugs. Accuracy and precision were acceptable according to international guidelines. Three cases tested positive for fentanyl and ketamine, in the setting of emergency room administration.CONCLUSIONS: The present methodology represents a fast, not expensive, wide-panel method for the analysis of more than 180 NPS by LC-MS/MS, which can be profitably applied both in a clinical context and in postmortem toxicology

Epigenetic Variability across Human Populations: A Focus on DNA Methylation Profiles of the KRTCAP3, MAD1L1 and BRSK2 Genes

Natural epigenetic diversity has been suggested as a key mechanism in microevolutionary processes due to its capability to create phenotypic variability within individuals and populations. It constitutes an important reservoir of variation potentially useful for rapid adaptation in response to environmental stimuli. The analysis of population epigenetic structure represents a possible tool to study human adaptation and to identify external factors that are able to naturally shape human DNA methylation variability. The aim of this study is to investigate the dynamics that create epigenetic diversity between and within different human groups. To this end, we first used publicly available epigenome-wide data to explore population-specific DNA methylation changes that occur at macro-geographic scales. Results from this analysis suggest that nutrients, UVA exposure and pathogens load might represent the main environmental factors able to shape DNA methylation profiles. Then, we evaluated DNA methylation of candidate genes (KRTCAP3, MAD1L1, and BRSK2), emerged from the previous analysis, in individuals belonging to different populations from Morocco, Nigeria, Philippines, China, and Italy, but living in the same Italian city. DNA methylation of the BRSK2 gene is significantly different between Moroccans and Nigerians (pairwise t-test: CpG 6 P-value = 5.2*10 (-) (3); CpG 9 P-value = 2.6*10 (-) (3); CpG 10 P-value = 3.1*10 (-) (3); CpG 11 P-value = 2.8*10 (-) (3)). Comprehensively, these results suggest that DNA methylation diversity is a source of variability in human groups at macro and microgeographical scales and that population demographic and adaptive histories, as well as the individual ancestry, actually influence DNA methylation profiles

From lifetime to evolution: Timescales of human gut microbiota adaptation

Human beings harbor gut microbial communities that are essential to preserve human health. Molded by the human genome, the gut microbiota (GM) is an adaptive component of the human superorganisms that allows host adaptation at different timescales, optimizing host physiology from daily life to lifespan scales and human evolutionary history. The GM continuously changes from birth up to the most extreme limits of human life, reconfiguring its metagenomic layout in response to daily variations in diet or specific host physiological and immunological needs at different ages. On the other hand, the microbiota plasticity was strategic to face changes in lifestyle and dietary habits along the course of the recent evolutionary history, that has driven the passage from Paleolithic hunter-gathering societies to Neolithic agricultural farmers to modern Westernized societie

Complex interplay between neutral and adaptive evolution shaped differential genomic background and disease susceptibility along the Italian peninsula

The Italian peninsula has long represented a natural hub for human migrations across the Mediterranean area, being involved in several prehistoric and historical population movements. Coupled with a patchy environmental landscape entailing different ecological/cultural selective pressures, this might have produced peculiar patterns of population structure and local adaptations responsible for heterogeneous genomic background of present-day Italians. To disentangle this complex scenario, genome-wide data from 780 Italian individuals were generated and set into the context of European/Mediterranean genomic diversity by comparison with genotypes from 50 populations. To maximize possibility of pinpointing functional genomic regions that have played adaptive roles during Italian natural history, our survey included also ∼250,000 exomic markers and ∼20,000 coding/regulatory variants with well-established clinical relevance. This enabled fine-grained dissection of Italian population structure through the identification of clusters of genetically homogeneous provinces and of genomic regions underlying their local adaptations. Description of such patterns disclosed crucial implications for understanding differential susceptibility to some inflammatory/autoimmune disorders, coronary artery disease and type 2 diabetes of diverse Italian subpopulations, suggesting the evolutionary causes that made some of them particularly exposed to the metabolic and immune challenges imposed by dietary and lifestyle shifts that involved western societies in the last centuries

Stochastic neutral modelling of the Gut Microbiota’s relative species abundance from next generation sequencing data

BACKGROUND: Interest in understanding the mechanisms that lead to a particular composition of the Gut Microbiota is highly increasing, due to the relationship between this ecosystem and the host health state. Particularly relevant is the study of the Relative Species Abundance (RSA) distribution, that is a component of biodiversity and measures the number of species having a given number of individuals. It is the universal behaviour of RSA that induced many ecologists to look for theoretical explanations. In particular, a simple stochastic neutral model was proposed by Volkov et al. relying on population dynamics and was proved to fit the coral-reefs and rain forests RSA. Our aim is to ascertain if this model also describes the Microbiota RSA and if it can help in explaining the Microbiota plasticity. RESULTS: We analyzed 16S rRNA sequencing data sampled from the Microbiota of three different animal species by Jeraldo et al. Through a clustering procedure (UCLUST), we built the Operational Taxonomic Units. These correspond to bacterial species considered at a given phylogenetic level defined by the similarity threshold used in the clustering procedure. The RSAs, plotted in the form of Preston plot, were fitted with Volkov’s model. The model fits well the Microbiota RSA, except in the tail region, that shows a deviation from the neutrality assumption. Looking at the model parameters we were able to discriminate between different animal species, giving also a biological explanation. Moreover, the biodiversity estimator obtained by Volkov’s model also differentiates the animal species and is in good agreement with the first and second order Hill’s numbers, that are common evenness indexes simply based on the fraction of individuals per species. CONCLUSIONS: We conclude that the neutrality assumption is a good approximation for the Microbiota dynamics and the observation that Volkov’s model works for this ecosystem is a further proof of the RSA universality. Moreover, the ability to separate different animals with the model parameters and biodiversity number are promising results if we think about future applications on human data, in which the Microbiota composition and biodiversity are in close relationships with a variety of diseases and life-styles

The Preliminary Design of a Volvo Open 70’.

I have followed the last Volvo Ocean Race during the first year of my study; I had a great interest to discover the rule that governed that great example of yacht design but I was only starting to get my first ideas on yacht design. With the oncoming of the new rule I have had a great opportunity to bring forward this design study and produce the preliminary design of a Volvo Open 70’

Novel Paradigms in the Cultural Heritage Digitization with Self and Custom-Built Equipment

In the field of the Cultural Heritage (CH), image-based 2D and 3D digital acquisition is today the most common technique used to create digital replicas of existing artifacts. This is carried out for many reasons, such as the following: research, analysis, preservation, conservation, communication, and valorization. These activities usually require complementary specialized equipment, tailored to specific purposes in order to achieve the desired results. This equipment is not easy-to-find on the market, it is not always affordable for museums operators; it is sometimes expensive, and it usually needs tricky customizations. However, the development in recent years of more generalized, versatile, and affordable instruments and technologies has led to new approaches, leveraging a new generation of low-cost, adaptable equipment. This paper presents custom-made equipment following this new path, designed to provide optimized results through calibrated tools alongside the software to make it work. The essay focuses specifically on the self-production of instruments for the digital reproduction of ancient drawings, manuscripts, paintings, and other museum artifacts and their transformative impact on digitization techniques. The outcomes of self and custom-built equipment specifically produced for the contexts described in this paper highlight their potential to foster interdisciplinary collaboration, facilitate scholarly research, enhance conservation efforts, and promote cultural exchange. The final goal is to propose inexpensive equipment that is easy to use (even by not specifically trained operators) and that provides remarkable quality

3-D Digital Modeling and Giuliano da Sangallo's Designs for Santa Maria delle Carceri in Prato

The paper introduces some studies on Santa Maria delle Carceri in Prato, one of the most representative central-plan churches of the Renaissance, which relies on a rich archival documentation made of an original drawing by Giuliano da Sangallo in the Taccuino Senese and several surveys of the today’s building. Introducing a specific methodology based on documents, reference studies and digital three-dimensional models, volumes and proportions of the church were investigated as a natural evolution of the design method followed by Giuliano. Several models have been authored, developing a described procedure, to end up with a digital reconstruction of the building as it could have been if the original idea expressed by Sangallo in the Taccuino would have been fulfilled

Visualization and Fruition of Cultural Heritage in the Knowledge-Intensive Society: New Paradigms of Interaction With Digital Replicas of Museum Objects, Drawings, and Manuscripts

The knowledge-intensive society paradigm fosters relationships between technology and human actors with data, values, and knowledge that become mutual drivers for social innovation. The cultural heritage sector is naturally influenced by this vision, and museums and cultural institutions have a prominent role in dissemination of cultural values. This chapter focuses on a method developed to combine the power of the computer visualization technology with the cultural elements spread across collections, introducing some notes and remarks on how digital replicas of drawings, manuscripts, and museum objects can be successfully employed to spread knowledge. Through a custom application called ISLe, aimed at visualizing 3D models that accurately replicate the original items, some experiences in the production of digital replicas are introduced, highlighting opportunities and criticalities to be considered in the adoption of technology that can be potentially shared and exploited by many possible figures involved in cultural heritage