Cardiopoietic cell therapy for advanced ischemic heart failure: results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial

Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort

The first derivative plot of raw intensities of individual molecules with respect to time.

<p>The activities of the enzymes are relatively stable between the heating pulses. The sudden increases of the activities observed in the graph are due to the heating pulse when the solution did not have time to reach room temperature. Those frames are excluded from the analysis.</p

Depiction of the energy landscape.

<p>An enzyme with a characteristic rate k₁ is trapped in a local minimum. When a heat pulse is introduced (ΔT), the enzyme changes its conformation and relaxes into a different minimum. The new conformation exhibits a different rate (k₂). After sequential pulses, the enzyme can adopt different conformations leading to different rates (k₃, k₄).</p

Bar graph representing the percentage of enzyme population gaining activity after each heating pulse.

<p>The error bars represent variations from four different experiments.</p

Histograms of activity distribution for single β-galactosidase molecules after each heating cycle.

<p>(A) Activity distribution of single β-galactosidase molecules before heating. The mean turnover number for the population of single β-galactosidase molecules of the enzyme population is calculated as 420 sec⁻¹. (B,C,D,E) Activity distribution of single enzyme molecules after each sequential heating pulse. The mean turnovers numbers are 426 sec⁻¹, 418 sec⁻¹, 427 sec⁻¹ and 411 sec⁻¹ respectively. The bin size for all the histograms is 50 sec⁻¹. Measurements were made at 20°C.</p

Activity traces and changes in individual β-galactosidase molecules between heat pulses.

<p>(A) Activity traces of all the individual β-galactosidase molecules taken from a single experiment. Six images were taken between each heating pulse and the activity was obtained (R² = 0.9 or greater). (B) Only four individual enzyme activity traces are shown to make it clear how the activities change after each heating pulse. The error bars correspond to linear fitting of data between heating pulses. (C, D, E, F) Histogram of the activity changes resulting from heat pulses. Negative values correspond to a decrease in the activity and positive values correspond to an increase in the activity after a heat pulse. The average changes in activity are 4 sec⁻¹, -10 sec⁻¹, 9 sec⁻¹, and -15 sec⁻¹ respectively for C, D, E and F.</p

Single enzymes trapped in wells and raw intensities of the trajectories.

<p>(A) Image of fiber bundle with trapped active enzymes. Each enzyme generates a high local concentration of fluorescent resorufin. (B) Raw fluorescence intensities of the trajectories of individual β-galactosidase molecules.</p

Observing Single Enzyme Molecules Interconvert between Activity States upon Heating

<div><p>In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules are trapped in femtoliter reaction chambers and the individual enzymes are subjected to short heating pulses. When heating pulses are introduced into the system, the enzyme molecules switch between different activity states. Furthermore, we observe that the changes in activity are random and do not correlate with the enzyme's original activity. This study demonstrates that different stable conformations play an important role in the static heterogeneity reported previously, resulting in distinct long-lived activity states of enzyme molecules in a population.</p></div

Observations of enzyme activities before and after heating.

<p>(A) Plot of the turnover number of enzymes before heating vs. turnover number after the fourth heating. No correlation between activities was observed. (B) Bar graph of sum of absolute activity changes for all enzymes after each heating pulse. No dramatic change is observed between different heating pulses.</p

Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.

The angiomotin (Amot)-Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remains unclear. We found that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. The conditional deletion of Amot and Yap1 in neurons led to a decrease in the complexity of Purkinje cell dendritic trees, abnormal cerebellar morphology, and impairments in motor coordination. Our results indicate that the function of Amot and Yap1 in dendrite growth does not rely on interactions with TEA domain (TEAD) transcription factors or the expression of Hippo pathway-dependent genes. Instead, Amot and Yap1 regulate dendrite development by affecting the phosphorylation of S6 kinase and its target S6 ribosomal protein