54 research outputs found
Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation
Generation and Analysis of Chromosomal Contact Maps of Bacteria
International audienceThis methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest
MatP-mediated positioning of chromosome terminus regions in
International audience is a curved rod-shaped bacterium with a single polar flagellum. Its genome is distributed on two circular chromosomes, ChrI and ChrII. Each carries a single origin of replication, and , withreplication terminating in a diametrically opposite zone, and . ChrII is derived from a megaplasmid. Its replication begins after duplication of , a sequence located in the middle of the left replication arm of ChrI, and ends at the same time as ChrI replication. Cell division is coordinatedwith the replication/segregation cycle by SlmA, an inhibitor of FtsZ-ring assembly that is bound all along ChrI and ChrII but and . and positioning is key to SlmA-mediated control of cell division. ChrI encodes a homologue of the MatP protein. MatP insulates the ter domain of the chromosome and dictate the positioning of sister ter copies at the centre of the cell by connecting them to the FtsZ-ring
Condensin- and replication-mediated bacterial chromosome folding and origin condensation revealed by Hi-C and super-resolution imaging
The organization and architecture of <em>Bacillus subtilis</em> chromosome revealed by super-resolution microscopy and HiC
Smart homes in 2012: when the floor becomes intelligent for people with loss of autonomy
International audienc
- …