Generation and Analysis of Chromosomal Contact Maps of Bacteria

International audienceThis methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest

MatP-mediated positioning of chromosome terminus regions in V. choleraeV.\ cholerae

International audience$V.\ cholerae$ is a curved rod-shaped bacterium with a single polar flagellum. Its genome is distributed on two circular chromosomes, ChrI and ChrII. Each carries a single origin of replication, $oriI$ and $oriII$, withreplication terminating in a diametrically opposite zone, $terI$ and $terII$. ChrII is derived from a megaplasmid. Its replication begins after duplication of $crtS$, a sequence located in the middle of the left replication arm of ChrI, and ends at the same time as ChrI replication. Cell division is coordinatedwith the replication/segregation cycle by SlmA, an inhibitor of FtsZ-ring assembly that is bound all along ChrI and ChrII but $terI$ and $terII$. $terI$ and $terII$ positioning is key to SlmA-mediated control of cell division. ChrI encodes a homologue of the $Escherichia\ coli$ MatP protein. $E.\ coli$ MatP insulates the ter domain of the $E.\ coli$ chromosome and dictate the positioning of sister ter copies at the centre of the cell by connecting them to the FtsZ-ring

Smart homes in 2012: when the floor becomes intelligent for people with loss of autonomy

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