1 research outputs found
Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets
KSRP is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for cellular proliferation and inflammatory response factors. The selectivity of this mRNA degradation mechanism relies on KSRP recognition of AU-rich elements in the mRNA 3â˛UTR, that is mediated by KSRPâs KH domains. Our structural analysis shows that the inter-domain linker orients the two central KH domains of KSRPâand their RNA-binding surfacesâcreating a two-domain unit. We also show that this inter-domain arrangement is important to the interaction with KSRPâs RNA targets