Effects of endurance racing on horse plasma extracellular particle miRNA

Background: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. Objectives: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. Study design: Cohort study. Methods: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. Results: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. Conclusions: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise

Uma proposta de modelagem da lista de materiais Bill of materials' modeling: a proposal

Este trabalho propõe uma nova forma de estruturação da lista de materiais, para empresas fabricantes de bens de capital, apesar dos conceitos gerais serem passíveis de utilização em outros tipos de companhias de manufatura. A lista de materiais, como elemento fomentador da integração, é modelada com base em necessidades-chave de diversos usuários, desde o orçamento até a expedição. O objetivo é facilitar as atividades de administração de materiais e gestão da produção. A metodologia desenvolvida consiste em estruturar uma lista de materiais paralela, delineada em função do organograma fabril da empresa e da seqüência de fabricação do produto no tempo. Esses conceitos foram aplicados na formação de grupos de dispositivos dedicados, utilizados na fabricação de hidrogeradores, na SADE VIGESA S/A., fábrica de Araraquara.<br>This paper suggests a new idea to form a Bill of Materials (BOM), for heavy equipment manufacturing companies, with the general concepts applied in others kind of manufacturing companies. The bill of materials, as an essential element of integration, from marketing to delivery, aims to facilitate the activities of Production Planning and of the Control System. The methodology developed is based on a new approach to build parallel bill of materials, using concepts of pseudo levels and assemblies, mainly based on the industrial organizational structure of the company and on the sequence of product fabrication in time. The implementation of this system for dedicated fixtures resulted in expressive reduction of lead time, project hours, fabrication hours and costs