Giant meningioma in paranasal sinuses: an atypical nasal occupation

info:eu-repo/semantics/publishedVersio

The gastropod statolith elemental composition by EPMA

Gastropods statoliths are biogenic structures of CaCO3, analogous to fish otoliths. Their application in sclerochronology is recent and may be advantageous comparing to the most traditional organisms used hitherto. Moreover, since elements bind within calcified structures, statoliths theoretically incorporate chemical information from the surrounding environment over time. So, besides being used for age estimation and growth studies, statoliths elemental fingerprinting is recognized as natural tag to track larval dispersal and may also be considered as a potential marker of environmental change and/or pollution. However there is a lack of information on the elemental composition of adult gastropod statoliths. Here we characterize Nassariusreticulatus (Gastropoda: Caenogastropoda) statoliths elemental composition by electron microprobe analysis (EMPA).One statolith from each of 3 adults was prepared for EMPA (Figure 1) and showed the same structural pattern: a nucleus circled by a metamorphic ring and, in addition to this core, 4 other rings delineating 5 increments (Figure 1F). General composition was assessed by Energy and Wavelength Dispersive Spectroscopy revealing C, Ca, O, Sr, Na, S and Mg as the most representative elements. These were then quantified in 3 points per increment. Carbon was not determined since surfaces were C-coated and the amount of oxides of Ca, Sr, Na, S and Mg was estimated from the valence. In addition, Ba – trace element in statoliths of some gastropods larvae – and Al, Cu, Hg, Sn – known as environmental pollutants – were also sought in one of the statoliths. For such, mean values for Ca, Sr, Na, S, Mg and O were set as the sample matrix. Standards and mean detection limits by element and statolith are indicated in Table 1.Concentrations of Ca, O, Na, Sr and S varied between 3.7x105-4.2x105, 1.5x105-1.7x105, 2.5x103-6.1x103, 1.3x103-8.9x103 and 4.7x102-1.3x103 ppm, respectively; Mg, which was not always detected, ranged from 2.0x102-8.6x102 ppm. No significant differences in these elements mean concentrations were found between increments, except Na in one statolith between increments 1 and 5 (higher at the edge; Dunn’s test s=2.014, p&gt;0.05). Thus the general elemental composition is nearly constant throughout lifespan (i.e. along statoliths radiuses). Regarding trace elements: Sn was never detected; Al and Hg were at 22.2% of the punctual analyses conducted while Cu was at 66.7% and Ba at 88.9%. Concentrations of Al, Hg, Cu and Ba varied between 55-71, 176-180, 183-302 and 647-969 ppm, respectively.EMPA proved to be a useful technique in sclerochronology, allowing the characterization of the overall composition of gastropods statoliths and the quantification of elements in specific locations with high resolution.This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the grant SFRH/BPD/70368/2010.</jats:p

When agriculture rules over the territory: Drystone walls

The objectives of this paper are mainly the dissemination of knowledge about drystone walls, a “minor heritage”, and the awareness of its importance in the territory characterization. These objectives are achieved when this theme is disclosed and explained. Heritage is protected and appreciated only if it is understood. The expected practical impact is essentially related to the recognition of this type of heritage. The main methodology consists of bibliographical and iconography collection, field surveys and exchange of ideas with the local people. The approach is made from the point of view of how the practiced agriculture characterizes the territory, not only its structure, because is also necessary to know the local typologies of drystone walls to allow to characterize them. Some characteristics of drystone walls were discovered in this investigation, which are directly related to the territory where they are inserted. The following limitations were found: the gradual replacement of drystone walls with masonry walls; changes in the type of agriculture; the difficulty of finding masters who have the know-how about drystone walls; the recognition of this heritage by the local population; and the gradual local population diminution. Practical implications are an improved sense of belonging and identity as the knowledge and appreciation of this heritage increases the pride of the population which was verified in the field surveys. The originality of this paper is the object of study, the Parish of Cernache do Bonjardim, Portugal, since there are very few works about it, also with added value to the dissemination of this type of heritage and its potential exploitation and protection by local population. It is possible to renew theterritory of these walls and use it for touristic, educational and cultural purposes, encouraging the local economy.info:eu-repo/semantics/publishedVersio

NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs

AbstractP75NTR is a type I integral membrane protein that plays a key role in neurotrophin signaling. However, structural data for the receptor in various functional states are sparse and controversial. In this work, we studied the spatial structure and mobility of the transmembrane and intracellular parts of p75NTR, incorporated into lipid-protein nanodiscs of various sizes and compositions, by solution NMR spectroscopy. Our data reveal a high level of flexibility and disorder in the juxtamembrane chopper domain of p75NTR, which results in the motions of the receptor death domain being uncoupled from the motions of the transmembrane helix. Moreover, none of the intracellular domains of p75NTR demonstrated a propensity to interact with the membrane or to self-associate under the experimental conditions. The obtained data are discussed in the context of the receptor activation mechanism

Structural Basis of p75 Transmembrane Domain Dimerization

Dimerization of single span transmembrane receptors underlies their mechanism of activation. p75 neurotrophin receptor plays an important role in the nervous system, but the understanding of p75 activation mechanism is still incomplete. The transmembrane (TM) domain of p75 stabilizes the receptor dimers through a disulfide bond, essential for the NGF signaling. Here we solved by NMR the three-dimensional structure of the p75-TM-WT and the functionally inactive p75-TM-C257A dimers. Upon reconstitution in lipid micelles, p75-TM-WT forms the disulfide-linked dimers spontaneously. Under reducing conditions, p75-TM-WT is in a monomer-dimer equilibrium with the Cys(257) residue located on the dimer interface. In contrast, p75-TM-C257A forms dimers through the AXXXG motif on the opposite face of the α-helix. Biochemical and cross-linking experiments indicate that AXXXG motif is not on the dimer interface of p75-TM-WT, suggesting that the conformation of p75-TM-C257A may be not functionally relevant. However, rather than mediating p75 homodimerization, mutagenesis of the AXXXG motif reveals its functional role in the regulated intramembrane proteolysis of p75 catalyzed by the γ-secretase complex. Our structural data provide an insight into the key role of the Cys(257) in stabilization of the weak transmembrane dimer in a conformation required for the NGF signaling.This work was supported in part by Russian Science Foundation Project 14-50-00131 (to A. S. A.) (NMR structural studies) and Spanish Ministry of Economy and Competitiveness (MINECO) Project BFU2013-42746-P (to M. V.). The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.S

The Synthetic Image TEsting Framework (SITEF) for the evaluation of multi-spectral image segmentation algorithms

ABSTRACT One of the most challenging tasks in Remote Sensing at present is how to handle the huge amounts of image data acquired every day by the existing Earth Observation Satellites (EOS). An alternative approach to the standard per-pixel analysis of multi-spectral EOS images has evolved over the last decade. Instead of focusing on individual image pixels, the object-based image analysis approach consists of partitioning an image into meaningful image-objects. One of the reasons for the development of object-based methods has been the dramatic increase in commercially available high resolution digital remote sensing imagery, with spatial resolutions of 5.0 m and finer [1]. Also it has been recognised that the image pixel is not a &quot;natural&quot; element of an image scene. A common element of all object-based image analysis systems is the segmentation stage, where the image is partitioned in a number of objects (or segments), which is clearly a critical stage of the whole process. If the segmentation fails to identify as an object a given element present in the image, the subsequent stages will generally be unable to recognise or to classify this element. An evaluation of the abilities and limitations of the segmentation algorithms used is therefore an important aspect of any object based image analysis system. However, there is no established standard procedure for the evaluation of the segmentation results produced for EOS images The purpose of this work is to present the Synthetic Image TEsting Framework (SITEF), a tool to evaluate the performance of segmentation algorithms on multi-spectral images. The method is based on the production of synthetic images with the spectral characteristics of the image pixels extracted from a signature multi-spectral image The methodology used here is an evolution of the method described in REFERENCES [1] G.J. Hay, G. Castilla, M.A. Wulder, J.R. Ruiz, &quot;An automated object-based approach for the multiscale image segmentation of forest scene