16 research outputs found
Pharmacodynamic basis of herbal medicine / Manuchair Ebadi.
pharmacy bookfair2015Includes bibliographical references (p. 661-672) and index.699 pages
Antisense Oligonucleotide-Mediated Inhibition of Metallothionein Protein Synthesis in Neuroblastoma IMR 32 and Chang Liver Cells in Culture
Patterns of Cellular Uptake and Effects on Cell Survival Using Antimetallothionein Oligodeoxyribonucleotide Conjugates in vitro
The modulatory effect of substance P on rat pineal norepinephrine release and melatonin secretion
The 27-kDa Heat Shock Protein Confers Cytoprotective Effects through a β2-Adrenergic Receptor Agonist-Initiated Complex with β-Arrestin
Heat shock proteins represent an emerging model for the coordinated,
multistep regulation of apoptotic signaling events. Although certain aspects
of the biochemistry associated with heat shock protein cytoprotective effects
are known, little information is found describing the regulation of heat shock
protein responses to harmful stimuli. During screening for noncanonical β
adrenergic receptor signaling pathways in human urothelial cells, using mass
spectroscopy techniques, an agonist-dependent interaction with β-arrestin
and the 27-kDa heat shock protein was observed in vitro. Formation of this
β-arrestin/Hsp27 complex in response to the selective β adrenergic
receptor agonist isoproterenol, was subsequently confirmed in situ by
immunofluorescent colocalization studies. Radioligand binding techniques
characterized a homogeneous population of the β2 adrenergic receptor
subtype expressed on these cells. Using terminal deoxynucleotidyl transferase
biotin-dUTP nick end labeling, immunoblot analysis and quantitation of
caspase-3 activity to detect apoptosis, preincubation of these cells with
isoproterenol was found to be sufficient for protection against programmed
cell death initiated by staurosporine. RNA interference strategies confirmed
the necessity for Hsp27 as well as both β-arrestin isoforms to confer
this cytoprotective consequence of β adrenergic receptor activation in
this cell model. As a result, these studies represent the first description of
an agonist-dependent relationship between a small heat shock protein and
β-arrestin to form a previously unknown antiapoptotic
“signalosome.