4,214 research outputs found
Metal cooldown, flow instability, and heat transfer in two-phase hydrogen flow
Studies of the properties of five metals with varying tube-wall thickness, with or without and internal coating of trifluorochloroethylene polymer, show that wall characteristics influence flow stability, affect heat transfer coefficients, and influence the transition point from dry- to wet-wall flow
Arginine mutation alters binding of a human monoclonal antibody to antigens linked to systemic lupus erythematosus and the antiphospholipid syndrome
Objective: Previous studies have shown the importance of somatic mutations and arginine residues in the complementarity-determining regions (CDRs) of pathogenic anti-double-stranded DNA (anti-dsDNA) antibodies in human and murine lupus, and in studies of murine antibodies, a role of mutations at position 53 in VH CDR2 has been demonstrated. We previously demonstrated in vitro expression and mutagenesis of the human IgG1 monoclonal antibody B3. The present study was undertaken to investigate, using this expression system, the importance of the arginine residue at position 53 (R53) in B3 VH.
Methods: R53 was altered, by site-directed mutagenesis, to serine, asparagine, or lysine, to create 3 expressed variants of VH. In addition, the germline sequence of the VH3-23 gene (from which B3 VH is derived) was expressed either with or without arginine at position 53. These 5 new heavy chains, as well as wild-type B3 VH, were expressed with 4 different light chains, and the resulting antibodies were assessed for their ability to bind to nucleosomes, -actinin, cardiolipin, ovalbumin, 2-glycoprotein I (2GPI), and the N-terminal domain of 2GPI (domain I), using direct binding assays.
Results: The presence of R53 was essential but not sufficient for binding to dsDNA and nucleosomes. Conversely, the presence of R53 reduced binding to -actinin, ovalbumin, 2GPI, and domain I of 2GPI. The combination B3 (R53S) VH/B3 VL bound human, but not bovine, 2GPI.
Conclusion: The fact that the R53S substitution significantly alters binding of B3 to different clinically relevant antigens, but that the alteration is in opposite directions depending on the antigen, implies that this arginine residue plays a critical role in the affinity maturation of antibody B3
Structure and magnetic properties of MnII[N(CN)2]2(pyrazine). An antiferromagnet with an interpenetrating 3-D network structure
Journal ArticleMn[N(CN)2]2(pyz) (pyz = pyrazine) orders antiferromagnetically at low temperature and possesses intralayer u-NCNCN and interlayer u-pyz ligands that form a pseudo-ReO3 interpenetrating network structure
Magnetic order in the quasi-one-dimensional spin 1/2 chain, copper pyrazine dinitrate
We present the first evidence of magnetic order in the quasi-one-dimensional
spin 1/2 molecular chain compound, copper pyrazine dinitrate Cu(C4H4N2)(NO3)2}.
Zero field muon-spin relaxation measurements made at dilution refrigerator
temperatures show oscillations in the measured asymmetry, characteristic of a
quasistatic magnetic field at the muon sites. Our measurements provide
convincing evidence for long range magnetic order below a temperature
T_N=107(1) mK. This leads to an estimate of the interchain coupling constant of
|J'|/k_B=0.046 K and to a ratio |J'/J| = 4.4 x 10^-3.Comment: 4 pages, 3 figures. Submitted to Physical Review Letter
Crystal structure and magnetic properties of [Fe{N(CN)2}2(MeOH)2] a 2-D layered network consisting of hydrogen-bonded 1-D chains
Journal ArticleA novel 2-D layered network structure [Fe{N(CN)2}2(MeOH)2] was synthesized and characterized by X-ray crystallography, vibrational, and magnetic susceptibility. The neutral 2-D stair-like framework consists of hydrogen-bonded infinite 1-D {Fe[N(CN)2]2} ribbons that pack in a staggered arrangement where nearest-neighboring chains are slipped a/2 relative to one another. Two methanol molecules are coordinated to the FeII center via the oxygen atoms in a trans configuration resulting in a compressed FeN4O2 octahedron. Hydrogen-bond interactions occur via N(2)...H(1)--O(1) where N(2) is the amide nitrogen atom of a nearby ribbon. The magnetic susceptibility was interpreted according to an S=2 expression which includes the Weiss constant and zero-field splitting giving g=2.04, 0=-2.0 K, and D=-1.7 K. Intrachain exchange interactions were determined from a fit to an S=2 antiferromagnetic chain model leading to g=2.04 and J/kB=0.23 K. Further interchain interaction via the hydrogen bond was determined by incorporation of a molecular-field correction term yielding J'/kB=-0.02 K indicating very weak antiferromagnetic coupling between chains
Interpenetrating three-dimensional rutile-like frameworks. Crystal structure and magnetic properties of MnII[C(CN)3]2
Journal ArticleThe interpenetrating double-density rutile-like structure and magnetic properties of MnII[C(CN)3]2 are determined
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