Hepatitis B vaccination status and knowledge, attitude, and practice regarding Hepatitis B among preclinical medical students of a medical college in Nepal.

BackgroundHepatitis B imposes a major public health problem with an increased risk of occupational exposure among unvaccinated health care workers. This study was conducted to determine the Hepatitis B vaccination status, along with the knowledge, attitude, and practice regarding Hepatitis B, among preclinical medical students of a medical college in Nepal.Materials and methodsThis descriptive study was conducted among preclinical students of a medical college in Kathmandu, Nepal from 6th July to 14th July 2020. The whole sampling technique was used. Data were collected using a pretested, self-administered questionnaire which was emailed to individuals and analyzed with the statistical package for social sciences version-22.ResultsA total of 181 students participated in the study out of 198, giving a response rate of 91.4%. Among the study participants, only 67 (37%) were fully vaccinated against Hepatitis B while 71 (39.2%) were never vaccinated. For the majority (74.6%) of the non-vaccinated participants, the main reason for not getting vaccination was a lack of vaccination programs. Half the study participants (n = 92, 50.8%) had good knowledge, attitude and practice regarding hepatitis B. The median knowledge, attitude and practice scores towards Hepatitis B were 61.00 (57.00-66.00), 20(18.00-21.00) and 21(19.00-23.00) respectively.ConclusionsThe majority of preclinical medical students were not fully vaccinated against Hepatitis B and only half of them had acceptable knowledge, attitude and practice towards Hepatitis B, which makes them vulnerable to the infection. This might represent the situation of not only Nepal, but also all South Asian countries, and creates concern about whether students take the vaccination programs seriously. Since unavailability of vaccination program is the main cause of non-vaccination, we strongly recommend the provision of the Hepatitis B vaccination program to the preclinical medical students

Benedikt syndrome in a 74‐year‐old hypertensive woman: A case report

Abstract Benedikt syndrome is a rare neurological disorder of the midbrain. Herein, we present a case of Benedikt syndrome, who presented with left‐sided body weakness, right oculomotor nerve palsy, cerebellar ataxia, and Holmes tremor in the left upper limb following midbrain infarction. She was treated with aspirin, clopidogrel, and amiodarone

Soil Fertility Assessment and Mapping of Agricultural Research Station, Jaubari, Illam, Nepal

Soil fertility evaluation is a prerequisite factor for sustainable planning of a particular region. Considering this, a study was conducted to determine the soil fertility status of the Agricultural Research Station, Jaubari, Illam, Nepal. In total, 78 soil samples were collected using soil sampling auger randomly from a depth of 0-20 cm. The texture, pH, OM, N, P2O5, K2O, Ca, Mg, S, B, Fe, Zn, Cu and Mn status of the samples were analyzed in the laboratory of Soil Science Division, Khumaltar by following standard analytical methods. The soil fertility maps of the observed parameters were prepared through Arc-GIS 10.1 software. The observed data revealed that soil was brown (10YR 4/3), dark grayish brown (10YR 4/2), dark yellowish brown (10YR 4/4) and yellowish brown (10YR 5/6) in colour, and the structure was granular. Similarly, the sand, silt and clay content were 53.84±1.06%, 34.34±0.83% and 11.82±0.47%, respectively and were indicated as sandy loam and loam in texture. The soil was very acidic (pH 3.85±0.04), and very low in available boron (0.26±0.06mg/kg) and available sulphur (0.59±0.15mg/kg). The available calcium (188.7±31.30mg/kg), available magnesium (50.98±5.0mg/kg) and available manganese (5.16±0.90mg/kg) were low. Likewise, available potassium (110.91±7.30mg/kg), available zinc (1.19±0.31mg/kg) and available copper (0.95±0.05mg/kg) content were medium. Similarly, organic matter (7.88±0.32%), total nitrogen (0.27±0.01%) and available phosphorus (36.53±5.66mg/kg) were high, and available iron (39.5±2.17 mg/kg) was very high.  International Journal of EnvironmentVolume-6, Issue-3, Jun-Aug 2017, page: 46-70</p

Prevalence and pattern of dyslipidemia in Nepalese individuals with type 2 diabetes

Abstract Background Atherogenic dyslipidemia is an important modifiable risk factor for cardiovascular disease among patients of type 2 diabetes mellitus. Timely detection and characterization of this condition help clinicians estimate future risk of cardiovascular disease and take appropriate preventive measures. The aim of this study was to determine the prevalence, pattern and predictors of dyslipidemia in a cohort of Nepalese patients with type 2 diabetes. Results We found mixed dyslipidemia as the most prevalent (88.1%) and isolated dyslipidemia (10.1%) as the least prevalent forms of dyslipidemia in our patients. The most prevalent form of single dyslipidemia was high LDL-C (73.8%) and combined dyslipidemia was high TG, high LDL-C and low HDL-C (44.7%). Prevalence of all single and mixed dyslipidemia was higher in patients with poor glycemic control and hypertension. The glycemic status of patients correlated with their fasting serum lipid profile. Dyslipidemia was associated mainly with male gender, poor glycemic control and hypertension. Conclusions Atherogenic dyslipidemia is associated mainly with male gender, poor glycemic control and hypertension. It is highly prevalent in Nepalese patients with type 2 diabetes. Urgent lifestyle modification, sustained glycemic control and aggressive lipid lowering treatment plans are necessary to minimize the future risk of cardiovascular disease in this population

MOESM1 of Prevalence and pattern of dyslipidemia in Nepalese individuals with type 2 diabetes

Additional file 1. Survey questionnaire and data collection form for screening dyslipidemia in Nepalese individuals with type 2 diabetes

Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal

Abstract Background Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. Resistance to antifungal agents has increased during the last decade. Thus, identification of Candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to speciate Candida species and to determine antifungal susceptibility pattern of Candida species to antifungal agents. Methods A total of 100 consecutive Candida species were isolated from 1248 clinical specimens over 7 months period. Growths on Sabouraud dextrose agar were evaluated for colony appearance, macroscopic examination, Gram staining, germ tube test and urea hydrolysis test. Further, they were processed for Candida speciation on CHROMagar. Antifungal susceptibility testing was performed as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document. Results Out of 100 Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans Candida species, Candida tropicalis (20%) was the predominant isolate followed by Candida glabrata (14%). Regarding antifungal susceptibility pattern, Candida species were more susceptible to clotrimazole (82%) followed by fluconazole (64%) and miconazole (44%). Conclusions Candida albicans was the predominant species responsible for various Candidal infections. Among commonly used antifungal drugs clotrimazole, miconazole and fluconazole were most effective

Draft genome sequence of an isolate of Extensively drug-resistant Mycobacterium tuberculosis from Nepal

Extensively drug-resistant (XDR) Mycobacterium tuberculosis has become a challenge to the treatment of tuberculosis (TB) in several countries, including Nepal. Here, we report for the first time the draft genome sequence of an isolate of XDR-TB collected in Nepal and describe single-nucleotide variations associated with its extensively drug-resistant phenotype