Pengaruh Lama Rebusan Daun Sirih terhadap Responsifitas Mastitis Sapi Perah

Mastitis is udder inflammation caused by bacteria that entry through the teat. Economically, mastitis is very detrimental to farmers because it can reduce milk production. The purpose of this study is to provide an alternative teat dipping solution that is safe, inexpensive, and easily found by the farmers. The research was carried out from September to October 2019 at the Claretian Benlutu Monastery Ranch, South Central Timor. The method used in this study is a randomized block design (CRD) consisting of 4 treatments 3 replications so that there are 12 experimental units. The treatments given are as follows R0 = plain water, R1 = commercial antiseptic solution, R2 = betel leaf decoction 45 minutes and R3 = decoction of betel leaf 60 minutes. The variables observed in this study were the result of mastitis detection and milk acidity test (pH test). The results showed that betel leaf stew did not have a significant effect on the results of the California Mastitis Test and the acidity level (pH). It can be concluded that the betel leaf decoction has the same ability as a commercial antiseptic in preventing mastitis in dairy cattle at Claretian Benlutu Ranch.Mastitis merupakan peradangan ambing yang dapat disebabkan oleh masuknya bakteri melalui lubang puting. Secara ekonomi, mastitis sangat merugikan peternak karena dapat menurunkan produksi susu. Tujuan dari penelitian ini untuk memberikan alternatif larutancelup puting yang aman, murah dan mudah ditemukan oleh masyarakat dan peternak. Penelitian ini dilaksanakan pada bulan September sampai Oktober 2019 di Peternakan Biara Claretian Benlutu, TimorTengahSelatan.Metode yang digunakan adalah Rancangan AcakLengkap (RAL) yang terdiri dari 4 perlakuan 3 ulangan sehingga terdapat 12 unit percobaan.Perlakuan yang diberikan adalah sebagai berikutR0=Air biasa, R1=Larutan antiseptik komersial, R2=Rebusandaunsirih 45 menit dan R3=Rebusandaunsirih 60 menit. Variabel yang diamatiadalahhasildeteksi mastitis dan uji derajatkeasaman susu (uji pH). Hasil penelitianmenunjukkan pemberian rebusan daun sirihmemberikan pengaruh yangtidak signifikan terhadap hasildeteksi mastitis dan tingkat keasaman (pH). Disimpulkan bahwa rebusan daun sirih mempunyai kemampuan yang sama dengan antisep komersil dalam mencegah terjadinya mastitis pada ternak sapi perah di Peternakan Claretian Benlutu

Randomly Diluted e_g Orbital-Ordered Systems

Dilution effects on the long-range ordered state of the doubly degenerate $e_g$ orbital are investigated. Quenched impurities without the orbital degree of freedom are introduced in the orbital model where the long-range order is realized by the order-from-disorder mechanism. It is shown by the Monte-Carlo simulation and the cluster-expansion method that a decrease in the orbital ordering temperature by dilution is remarkable in comparison with that in the randomly diluted spin models. Tiltings of orbitals around impurity cause this unique dilution effects on the orbital systems. The present theory provides a new view point for the recent experiments in KCu$_{1-x}$Zn$_x$F$_3$.Comment: 4 pages, 4 figure

pH responsive biomineralization onto chitosan grafted biodegradable substrates

Bioactive composites that enable the formation of an apatite layer onto the surface are important in the development of osteoconductive biomaterials in orthopaedic applications. In this work, the surface of biodegradable and bioactive substrates, composed of poly(L-lactic acid) (PLLA) reinforced with Bioglass , was modified by coupling chitosan to the surface, using plasma activation. The wettability of the modified films was analysed by contact angle (CA) measurements as a function of pH. It was found that this surface property changed significantly with pH. Moreover, the apatite formation on the surface upon immersion of the modified films in a simulated body fluid (SBF) solution was analysed at pH 5.4 and pH 7.4 by scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS). It was found that such modification, together with the effect of pH, could block the formation of apatite onto the biodegradable substrate upon immersion in a simulated body fluid solution when the pH changed to 5.4. On the other hand, a dense apatite layer was formed at pH 7.4. For the unmodified substrates an apatite layer was formed at both pHs. These results suggest that the formation of apatite or possibly other kinds of minerals could be controlled by such a smart surface, in this case pH-responsive

Effect of The Inclusion of Butterfly Pea (Clitoria ternatea) and Green Sea Weed (Ulva lactuca) in The Complete Feed on The Concentration of Blood Metabolites of Male Kacang Goats

This experiment aimed to investigate the effect of including  Clitoria ternatea and Ulva lactuca in the complete feed on the concentration of blood metabolites of male kacang goats. Four male kacang goats were involved in this experiment arranged following a Latin Square Design with 4 treatments and 4 periods as replication. The treatments were R0= native grass hay 60%+concentrate 40%, R1= native grass hay 30%+concentrate 40%+ Clitoria ternatea 30%, R2= native grass hay 30%+concentrate 40%+ Clitoria ternatea 15%+ Ulva lactuca 15% and R3= native grass hay 30%+ concentrate 40%+Ulva lactuca 30%. Data were analyzed using analysis of variance (ANOVA) and followed by the Duncan Multiple Range Test when significant differences were found. The results showed that  feeding a complete feed containing  Clitoria ternatea and Ulva lactuca did not significantly (P>0,05) affect the concentration of urea, glucose and  plasm total protein in the blood of male kacang goats. Therefore, it can be concluded the inclusion of Clitoria ternatea and Ulva lactuca has no effect on the concentration of blood metabolites in male kacang goats

Two-dimensional open microfluidic devices by tuning the wettability on patterned superhydrophobic polymeric surface

We present a simple and economical method to produce a potential open microfluidic polymeric device. Biomimetic superhydrophobic surfaces were prepared on polystyrene using a phase separation methodology. Patterned two-dimensional channels were imprinted on the superhydrophobic substrates by exposing the surface to plasma or UV–ozone radiation. The wettability of the channels could be precisely controlled between the superhydrophobic and superhydrophilic states by changing the exposure time. The ability of superhydrophilic paths to drive liquid flows in a horizontal position was found to be significantly higher than for the case of hydrophilic paths patterned onto smooth surfaces.(undefined

High-throughput evaluation of interactions between biomaterials, proteins and cells using patterned superhydrophobic substrates

We propose a new low cost platform for high-throughput analysis that permits screening the biological performance of independent combinations of biomaterials, cells and culture media. Patterned superhydrophobic flat substrates with controlled wettable spots are used to produce microarray chips for accelerated multiplexing evaluation.This work was partially supported by Fundação para a Ciência e Tecnologia (FCT) under project PTDC/FIS/68517/2006

Combinatorial analysis of marine based biomaterials: high-throughput analysis of the effect of nanostructured multilayers on cell behaviour

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface allowing generate irreversible bonding. Adhesive secreted proteins of mussels present an unusual amino acid 3,4-dihydroxyphenylalanine (DOPA). Inspired by the struc- ture and properties of mussel adhesive proteins, layer-by-layer (LbL) coatings based on polymers that contain catechol groups were devel- oped. We used dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry to form thin and surface-adherent dopamine films. The multilayer films were developed by electrostatic interactions using chitosan (CHT) as polycation and HA-DN as polyanion. Multilay- ers films of CHT and HA were used as control. The formation of these films was investigated in-situ by quartz crystal microbalance with dissi- pation monitoring (QCM-D). Afterwards, many combinations of the marine inspired biomaterials were analysed in a high-throughput (HTS) way. Such multilayers were constructed and individually dis- posed on isolated transparent spots, patterned onto biomimetic super- hydrophobic substrates. The adhesion properties of the coatings in the chips were also analyzed. In vitro tests using two distinct cell sources were carried out to evaluate the biological performance of the different combinations of multilayers that could be useful in different biomedical applications, including tissue engineering

Layer-by-layer deposition of antimicrobial polymers on cellulosic fibers : a new strategy to develop bioactive textiles

In recent years, there has been an increase of infectious diseases caused by different microorganisms and the development of antibiotic resistance. In this way, the search for new and efficient antibacterial materials is imperative. The main polysaccharides currently used in the biomedical and pharmaceutical domains are chitin and its derivative chitosan (CH) and alginates (ALG). In this study, a simple technique of Layer by Layer (LbL) of applying polycation CH and polyanion ALG was used to prepare CH/ALG multilayers on cotton samples via the electrostatic assembly with success. The CH/ALG cotton samples (functionalized) were investigated for their antibacterial properties towards Staphylococcus aureus and Klebsiella pneumonia using the international standard method JIS L 1902:2002. The antibacterial activity of the functionalized samples was tested in terms of bacteriostatic and bactericidal activity, and results showed that the samples exhibited a bacteriostatic effect on the two bacteria tested, as expected. In addition, samples with five layers (CH/ALG/CH/ALG/CH) were more effective in inhibiting bacterial growth. This new coating for cellulosic fibers is a new strategy and may open new avenues for the development of antimicrobial polymers with potential application in health-care field.The authors would like to thank Fundacao para a Ciencia e Tecnologia (FCT) for the funding granted concerning the project - PTDC/EBB-BIO/113671/2009 (FCOMP-01-0124-FEDER-014752) Skin2Tex. Also, we would like to thank Fundo Europeu de Desenvolvimento Regional (FEDER) through COMPETE - Programa Operacional Factores de Competitividade (POFC) for the co-funding

Wettable arrays onto superhydrophobic surfaces for bioactivity testing of inorganic nanoparticles

Poly(l-lactic acid) superhydrophobic surfaces prepared by a phase-separation methodology were treated with 30 min exposition of UV/O3 irradiation using hollowed masks in order to obtain patterned superhydrophilic squared-shaped areas. These wettable areas successfully confined bioactive glass nanoparticles (BG-NPs), by dispensing and drying individual droplets of BG-NPs suspensions. The obtained biomimetic chips were used to test the in vitro bioactivity of binary (SiO2–CaO) and ternary (SiO2–CaO–P2O5) nanoparticles produced using sol–gel chemistry by immersing such substrate in simulated body fluid (SBF). From SEM and EDX it was possible to conclude that the ternary system promoted an enhanced apatite deposition. This work shows the potential of using such flat disposable matrices in combinatory essays to easily evaluate the osteoconductive potential of biomaterials using small amounts of different samples.Fundação para a Ciência e a Tecnologia (FCT) - PTDC/QUI/69263/2006