260 research outputs found
Vibrational and electronic entropy of β-cerium and γ-cerium measured by inelastic neutron scattering
Time-of-flight (TOF) inelastic neutron-scattering spectra were measured on β-cerium (double hcp) and γ-cerium (fcc) near the phase-transition temperature. Phonon densities of states (DOS) and crystal-field levels were extracted from the TOF spectra. A softening of the phonon DOS occurs in the transition from β- to γ-cerium, accounting for an increase in vibrational entropy of ΔSvibγ-β=(0.09±0.05)kB/atom. The entropy calculated from the crystal-field levels and a fit to calorimetry data from the literature were significantly larger in β-cerium than in γ-cerium below room temperature, but the difference was found to be negligible at the experimental phase-transition temperature. A contribution to the specific heat from Kondo spin fluctuations was consistent with the quasielastic magnetic scattering, but the difference between phases was negligible. To be consistent with the latent heat of the β-γ transition, the increase in vibrational entropy at the phase transition may be accompanied by a decrease in electronic entropy not associated with the crystal-field splitting or spin fluctuations. At least three sources of entropy need to be considered for the β-γ transition in cerium
Deficits in face recognition and consequent quality-of-life factors in individuals with cerebral visual impairment
A Scalable Correlator Architecture Based on Modular FPGA Hardware, Reuseable Gateware, and Data Packetization
A new generation of radio telescopes is achieving unprecedented levels of
sensitivity and resolution, as well as increased agility and field-of-view, by
employing high-performance digital signal processing hardware to phase and
correlate large numbers of antennas. The computational demands of these imaging
systems scale in proportion to BMN^2, where B is the signal bandwidth, M is the
number of independent beams, and N is the number of antennas. The
specifications of many new arrays lead to demands in excess of tens of PetaOps
per second.
To meet this challenge, we have developed a general purpose correlator
architecture using standard 10-Gbit Ethernet switches to pass data between
flexible hardware modules containing Field Programmable Gate Array (FPGA)
chips. These chips are programmed using open-source signal processing libraries
we have developed to be flexible, scalable, and chip-independent. This work
reduces the time and cost of implementing a wide range of signal processing
systems, with correlators foremost among them,and facilitates upgrading to new
generations of processing technology. We present several correlator
deployments, including a 16-antenna, 200-MHz bandwidth, 4-bit, full Stokes
parameter application deployed on the Precision Array for Probing the Epoch of
Reionization.Comment: Accepted to Publications of the Astronomy Society of the Pacific. 31
pages. v2: corrected typo, v3: corrected Fig. 1
Purification and characterization of platelet aggregation inhibitors from snake venoms
Proteins that inhibit glycoprotein (GP) IIb/IIIa mediated platelet aggregation have been purified from the venom of two snake species. A small platelet aggregation inhibitor (pl.AI), multisquamatin (Mr=5,700), was purified from Echis multisquamatus venom by hydrophobic interaction HPLC and two steps on C18 reverse phase HPLC. A larger pl.AI, contortrostatin (Mr=15,000), was purified by a similar HPLC procedure from the venom of Agkistrodon contortrix contortrix. Both pl.AIs inhibit ADP-induced human, canine and rabbit platelet aggregation using platelet rich plasma (PRP). Multisquamatin has an IC50 of 97 nM, 281 nM and 333 nM for human, canine and rabbit PRP, respectively. Contortrostatin has an IC50 of 49 nM, 120 nM and 1,150 nM for human, canine and rabbit PRP, respectively. In a competitive binding assay using 125I-7E3 (a monoclonal antibody to GPIIb/IIIa that inhibits platelet aggregation) both contortrostatin and multisquamatin demonstrated GPIIb/IIIa specific binding to human and canine platelets. The IC50 for contortrostatin displacement of 7E3 binding to human and canine GPIIb/IIIa is 27 nM and 16 nM, respectively and for multisquamatin it is 3 nM and 63 nM, respectively. Our results indicate that both pl.AIs inhibit platelet aggregation by binding with high affinity to GPIIb/IIIa.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31863/1/0000813.pd
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms. Application of these methodologies to understanding processes underlying DNA replication and repair has been limited to defined in vitro biochemical analysis and prokaryotic cells. In order to expand these techniques to eukaryotic systems, we have further developed a photo-activated localization microscopy-based method to directly visualize DNA-associated proteins in unfixed eukaryotic cells. We demonstrate that motion blurring of fluorescence due to protein diffusivity can be used to selectively image the DNA-bound population of proteins. We designed and tested a simple methodology and show that it can be used to detect changes in DNA binding of a replicative helicase subunit, Mcm4, and the replication sliding clamp, PCNA, between different stages of the cell cycle and between distinct genetic backgrounds
Elastically driven, intermittent microscopic dynamics in soft solids
Soft solids with tunable mechanical response are at the core of new material
technologies, but a crucial limit for applications is their progressive aging
over time, which dramatically affects their functionalities. The generally
accepted paradigm is that such aging is gradual and its origin is in slower
than exponential microscopic dynamics, akin to the ones in supercooled liquids
or glasses. Nevertheless, time- and space-resolved measurements have provided
contrasting evidence: dynamics faster than exponential, intermittency, and
abrupt structural changes. Here we use 3D computer simulations of a microscopic
model to reveal that the timescales governing stress relaxation respectively
through thermal fluctuations and elastic recovery are key for the aging
dynamics. When thermal fluctuations are too weak, stress heterogeneities
frozen-in upon solidification can still partially relax through elastically
driven fluctuations. Such fluctuations are intermittent, because of strong
correlations that persist over the timescale of experiments or simulations,
leading to faster than exponential dynamics.Comment: 7 pages, Supplementary Information include
Comparison of IFCC-calibrated HbA1c from laboratory and point of care testing systems
Objective: WHO, IDF and ADA recommend HbA1c ≥6.5% (48 mmol/mol) for diagnosis of diabetes with pre-diabetes 6.0% (42 mmol/mol) [WHO] or 5.7% (39 mmol/mol) [ADA] to 6.4% (47 mmol/mol). We have compared HbA1c from several methods for research relating glycaemic markers.Research design and methods: HbA1c was measured in EDTA blood from 128 patients with diabetes on IE HPLC analysers (Bio-Rad Variant II NU, Menarini HA8160 and Tosoh G8), point of care systems, POCT, (A1cNow+ disposable cartridges and DCA 2000®+ analyser), affinity chromatography (Primus Ultra2) and the IFCC secondary reference method (Menarini HA8160 calibrated using IFCC SRM protocol).Results: median (IQ range) on IFCC SRM was 7.5% (6.8–8.4) (58(51–68) mmol/mol) HbA1c with minimum 5.3%(34 mmol/mol)/maximum 11.9%(107 mmol/mol). There were positive offsets between IFCC SRM and Bio-Rad Variant II NU, mean difference (1SD), +0.33%(0.17) (+3.6(1.9) mmol/mol), r2 = 0.984, p < 0.001 and Tosoh G8, +0.22%(0.20) (2.4(2.2) mmol/mol), r2 = 0.976, p < 0.001 with a very small negative difference −0.04%(0.11) (−0.4(1.2) mmol/mol), r2 = 0.992, p < 0.001 for Menarini HA8160. POCT methods were less precise with negative offsets for DCA 2000®+ analyser −0.13%(0.28) (−1.4(3.1) mmol/mol), r2 = 0.955, p < 0.001 and A1cNow+ cartridges −0.70%(0.67) (−7.7(7.3) mmol/mol), r2 = 0.699, p < 0.001 (n = 113). Positive biases for Tosoh and Bio-Rad (compared with IFCC SRM) have been eliminated by subsequent revision of calibration.Conclusions: small differences observed between IFCC-calibrated and NGSP certified methods across a wide HbA1c range were confirmed by quality control and external quality assurance. As these offsets affect estimates of diabetes prevalence, the analyser (and calibrator) employed should be considered when evaluating diagnostic data.</p
Nilotinib and Imatinib Are Comparably Effective in Reducing Growth of Human Eosinophil Leukemia Cells in a Newly Established Xenograft Model
We developed a xenograft model of human Chronic Eosinophilic Leukemia (CEL) to study disease progression and remission-induction under therapy with tyrosine kinase inhibitors using imatinib and nilotinib as examples. The FIP1L1/PDGFRA+ human CEL cell lineEOL-1 was injected intravenously into scid mice, and MR imaging and FACS analysis of mouse blood samples were performed to monitor disease development and the effects of imatinib and nilotinib. Organ infiltration was analyzed in detail by immunohistochemistry after sacrifice. All animals developed CEL and within one week of therapy, complete remissions were seen with both imatinib and nilotinib, resulting in reduced total tumor volumes by MR-imaging and almost complete disappearance of EOL-1 cells in the peripheral blood and in tissues. The new model system is feasible for the evaluation of new tyrosine kinase inhibitors and our data suggest that nilotinib may be a valuable additional targeted drug active in patients with FIP1L1/PDGFRA+ CEL
Vibrational and electronic entropy of β-cerium and γ-cerium measured by inelastic neutron scattering
The western Arctic boundary current at 152°W : structure, variability, and transport
Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Deep Sea Research Part II: Topical Studies in Oceanography 56 (2009): 1164-1181, doi:10.1016/j.dsr2.2008.10.014.From August 2002 to September 2004 a high-resolution mooring array was maintained across the western Arctic boundary current in the Beaufort Sea north of Alaska. The array consisted of profiling instrumentation, providing a timeseries of vertical sections of the current. Here we present the first-year velocity measurements, with emphasis on the Pacific water component of the current. The mean flow is characterized as a bottom-intensified jet of O(15 cm s-1) directed to the east, trapped to the shelfbreak near 100 m depth. Its width scale is only 10-15 km. Seasonally the flow has distinct configurations. During summer it becomes surface-intensified as it advects buoyant Alaskan Coastal Water. In fall and winter the current often reverses (flows westward) under upwelling-favorable winds. Between the storms, as the eastward flow re-establishes, the current develops a deep extension to depths exceeding 700 m. In spring the bottom-trapped flow advects winter-transformed Pacific water emanating from the Chukchi Sea. The year-long mean volume transport of Pacific Water is 0.13±0.08 Sv to the east, which is less than 20% of the long-term mean Bering Strait inflow. This implies that most of the Pacific water entering the Arctic goes elsewhere, contrary to expected dynamics and previous modeling results. Possible reasons for this are discussed. The mean Atlantic water transport (to 800 m depth) is 0.047±0.026 Sv, also smaller than anticipated.AN was funded by the Swedish Research Council; RP, PF, and DT were funded by grant N00014-02-1-0317 of the Office of Naval Research
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