Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)

A new system, the Toxorhynchites-fluorescent antibody (TFA) test in which the larvae of Toxorhynchites splendens mosquitoes were used for the detection of bluetongue virus (BTV) from Culicoides midges, was developed. Twenty-seven pools of Culicoides midges were collected from bluetongue-prone areas of Tamil Nadu by use of the light-trap and suction-trap methods. A suspension of each pool was injected intrathoracically into T. splendens IV instar larvae and inoculated onto Vero cell monolayers. An indirect fluorescent antibody technique and an immunoperoxidase test were used to detect BTV antigen in smears of crushed midges, crushed larval head smears after incubation for 7 d at 28° and cell monolayers showing cytopathic effects 48 h post inoculation. The suspensions were also injected intravenously into embryonated chicken eggs, and the characteristic BTV-induced lesion(s), viz. cherry-red appearance of embryos, were observed after 48 h. Virus was confirmed by a qualitative neutralization test conducted simultaneously in embryonated chicken eggs. A total of seven out of 27 samples (26%) were positive for the presence of BTV antigen in all the diagnostic systems used. Since BTV propagates readily in experimentally infected T. splendens larvae and the BTV antigen can be detected by the fluorescent antibody technique with a sensitivity comparable to that for virus propagated in tissue culture and embryonated eggs, the TFA system can be adopted as a new method for the isolation of BTV from vectors. The advantages of the TFA system are discussed.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

On the source of QPO of the black hole candidate GRS1915+105: some new observations and their interpretation

A few classes of the light curve of the black hole candidate GRS 1915+105 have been analyzed in detail. We discover that unlike the previous findings, QPOs occasionally occur even in the so-called `On' or softer states. Such findings may require a revision of the accretion/wind scenario of the black hole candidates. We conjecture that considerable winds which is produced in `Off' states, cool down due to Comptonization, and falls back to the disk and creating an excess accretion rate to produce the so-called `On' state. After the drainage of the excess matter, the disk goes back to the `Off' state. Our findings strengthen the shock oscillation model for QPOs.Comment: MNRAS style. MNRAS (To appear

Comparing the influence of natural farming on cotton (Gossypium hirsutum) yield and economics with integrated and organic farming

Cotton, a vital global cash crop, influences the economy and sustainability. Natural farming is a cost-effective, eco-friendly method. This study examines Natural Farming's effects on cotton yield and the economics of cotton compared to Integrated and organic farming. Field experiments were conducted during 2021-22 and 2022-23 at Tamil Nadu Agricultural College, Coimbatore. The experiment consisted of eight treatments, laid out in Randomized Block Design and replicated thrice. The treatments consist of control (T1), various Indian Natural farming practices including Beejamirit as a seed treatment, Ghanajeevamirit as basal, Jeevamirit with irrigation water, crop residue mulch, intercroping with pulses (T2 to T5), Organic farming practices (T6), Integrated crop management (ICM) practices with organic pest control (T7) and ICM with chemical pest (T8). The results indicated a significant increase in yield components: sympodial branches plant-1 (12.85 and 13.40), number of squares (29.28 and 31.28), bolls plant-1 (10.74 and 11.32), and boll weight (4.30 and 4.38 g) under Integrated Crop Management practices in 2021-22 and 2022-23, respectively. However, organic farming practices, followed by natural farming practices, were at par with integrated crop management. Integrated crop management practices resulted in significantly higher seed cotton yields (2178 and 2232 kg/ha in 2021-22 and 2022-23, respectively) than organic and natural farming practices with a higher cost of cultivation and a gross return. Complete natural farming outperformed organic and integrated farming in cotton, yielding higher net returns and B: C ratios. This approach holds promise for enhancing yield, economic viability and environmental sustainability in cotton cultivation

Generalized Kac-Moody Algebras from CHL dyons

We provide evidence for the existence of a family of generalized Kac-Moody(GKM) superalgebras, G_N, whose Weyl-Kac-Borcherds denominator formula gives rise to a genus-two modular form at level N, Delta_{k/2}(Z), for (N,k)=(1,10), (2,6), (3,4), and possibly (5,2). The square of the automorphic form is the modular transform of the generating function of the degeneracy of CHL dyons in asymmetric Z_N-orbifolds of the heterotic string compactified on T^6. The new generalized Kac-Moody superalgebras all arise as different `automorphic corrections' of the same Lie algebra and are closely related to a generalized Kac-Moody superalgebra constructed by Gritsenko and Nikulin. The automorphic forms, Delta_{k/2}(Z), arise as additive lifts of Jacobi forms of (integral) weight k/2 and index 1/2. We note that the orbifolding acts on the imaginary simple roots of the unorbifolded GKM superalgebra, G_1 leaving the real simple roots untouched. We anticipate that these superalgebras will play a role in understanding the `algebra of BPS states' in CHL compactifications.Comment: LaTeX, 35 pages; v2: improved referencing and discussion; typos corrected; v3 [substantial revision] 44 pages, modularity of additive lift proved, product representation of the forms also given; further references adde

Maximum Power Point Tracking for Cascaded PV-Converter Modules Using Two-Stage Particle Swarm Optimization

The paper presents a novel two-stage particle swarm optimization (PSO) for the maximum power point tracking (MPPT) control of a PV system consisting of cascaded PV-converter modules, under partial shading conditions (PSCs). In this scheme, the grouping method of the shuffled frog leaping algorithm (SFLA) is incorporated with the basic PSO algorithm, ensuring fast and accurate searching of the global extremum. An adaptive speed factor is also introduced to improve its convergence speed. A PWM algorithm enabling permuted switching of the PV sources is applied. The method enables this PV system to achieve the maximum power generation for any number of PV and converter modules. Simulation studies of the proposed MPPT scheme are performed on a system having two chained PV buck-converter modules and a dc-ac H-bridge connected at its terminals for supplying an AC load. The results show that this type of PV system allows each module to achieve the maximum power generation according its illumination level without affecting the others, and the proposed new control method gives significantly higher power output compared with the conventional P&O and PSO methods

How Do Black Holes Predict the Sign of the Fourier Coefficients of Siegel Modular Forms?

Single centered supersymmetric black holes in four dimensions have spherically symmetric horizon and hence carry zero angular momentum. This leads to a specific sign of the helicity trace index associated with these black holes. Since the latter are given by the Fourier expansion coefficients of appropriate meromorphic modular forms of Sp(2,Z) or its subgroup, we are led to a specific prediction for the signs of a subset of these Fourier coefficients which represent contributions from single centered black holes only. We explicitly test these predictions for the modular forms which compute the index of quarter BPS black holes in heterotic string theory on T^6, as well as in Z_N CHL models for N=2,3,5,7.Comment: LaTeX file, 17 pages, 1 figur

Spectral Signature of Wind Generation From The Post-Shock Region in GRS1915+105 Accretion Disk

Accretion and outflows are common in systems which include black holes. Especially important is the case of the well known micro-quasar GRS1915+105 in our own galaxy, where super-luminal outflows are detected. We present a few observation which are suggestive of an outflow which is generated very close to the black hole, within a few tens of Schwarzschild radii. In the presence of mass loss (e.g., an outflowing wind), the electron density of matter within the centrifugal pressure supported region (which generates hard X-rays) goes down and it is easier to cool these electrons by soft photons coming from the Keplerian disk. If, on the other hand, the post-shock region gains mass from outside, the spectra would be harder. These properties of spectral `softening' of the low state and `hardening' of the high state have been detected in several days of RXTE data of GRS1915+105 which we present here.Comment: 10 latex pages with four figures (Submitted Astronomy and Astrophysics, May 2000

How do Muslim service users, caregivers, and community members in Malappuram, Kerala, use their faith to address the challenges associated with mental ill health?

Our aim was to explore the role religion and spiritual beliefs play in dealing with the challenges associated with mental ill health among the Muslim community in Malappuram, Kerala. Twenty-four interviews were conducted with patients of Islamic faith diagnosed with a mental health condition (n = 10) in urban (Ponnani) and rural (Vailathur) area of Malappuram, a Muslim majority district in Kerala, their family carers (n = 8) and community members (n = 6). Four key themes were derived, namely (1) Attribution to supernatural factors, (2) Relying on “God’s will”, (3) Prayer, and (4) Traditional healing. Faith was seen to be a prerequisite for any treatment, including modern medicine, to work. Even within a single faith group there can be considerable variation in belief and practice, with more pious participants disapproving of the reliance on local traditional healers and belief systems, highlighting the value of paying attention to the detail of local beliefs and practices

Syntaxin 16 is a master recruitment factor for cytokinesis

Recently it was shown that both recycling endosome and endosomal sorting complex required for transport (ESCRT) components are required for cytokinesis, in which they are believed to act in a sequential manner to bring about secondary ingression and abscission, respectively. However, it is not clear how either of these complexes is targeted to the midbody and whether their delivery is coordinated. The trafficking of membrane vesicles between different intracellular organelles involves the formation of soluble N-ethylmalei­mide–sensitive factor attachment protein receptor (SNARE) complexes. Although membrane traffic is known to play an important role in cytokinesis, the contribution and identity of intracellular SNAREs to cytokinesis remain unclear. Here we demonstrate that syntaxin 16 is a key regulator of cytokinesis, as it is required for recruitment of both recycling endosome–associated Exocyst and ESCRT machinery during late telophase, and therefore that these two distinct facets of cytokinesis are inextricably linked