Changes in the protein content of Lamellidens marginalis from Jayakwadi dam at paithan during different seasons (M.S) India

In the present study, variation in the protein content in soft body tissues of Lamellidensmarginalis were collected from Jayakwadi dam, at Paithan near Aurangabad was observed during different seasons. As variation in the environmental conditions, it shown an effect on protein contents in the tissues like, mantle, hepatopancreas, gonad and foot. Protein content maximum found in gonads throughout all the three seasons, whereas mantle shows minimum values of protein. There are great fluctuations in the values of protein during different seasons

Energy Metering by using Power Line Communication

Everywhere automation is needed to reduce the work. We decided to put in to practice the meter reading using power line communication. It can be measure real time and also save the time. It had better user interface and digital data analysis. Data was send over existing carrier by using power line that's minimizes the complexity and cost of system. Energy meter reading was a monotonous and costly work. The meter reader people run through each meter and catch the meter reading manually to issue the bill which will later be entered in the billing software for payment automation. If the manual meter reading and bill data entry process can be automated then it minimizes the hard task and financial wastage system. It was used for data collecting from the meter and processing the collected data for billing and other decision purposes. We had proposed an automatic meter reading system which was low cost, high performance and cover highest coverage area. In the data receiving and processing unit meter reading was collected from the transceiver which controlled by another microcontroller. There was computer application that will take the data from the microcontroller. This was help to avoid any tampering or break down of energy. DOI: 10.17762/ijritcc2321-8169.15027

HPLC ANALYSIS OF HUMAN URINE FOR OXALATE CONTENT

Objective: In the present communication, development and validation of reverse phase-high performance liquid chromatography method have been carried out for estimation of oxalate content in the urine of human volunteers with recurrent kidney stone disease and healthy status.Methods: The analysis of oxalic acid has been carried out on KYA TECH HiQ Sil C18HS column using a mobile phase of methanol: 0.001 N acetic acid in water (50:50, v/v) with a flow rate of 1 ml/min and detection wavelength, 237 nm.Results: Analysis of oxalate content was carried out using single point calibration method with retention at 2.705 min with good resolution parameters. Urine sample collected from kidney stone patients and healthy volunteers over the period of 24 h were analyzed and it has been found that concentration of oxalate in healthy volunteers is less than 12 µg/ml whereas that in case of kidney stone patients is in the range of 39-151 µg/ml and this data can be utilized for further interpretations about oxalate content in healthy and kidney stone diseased volunteers. This method was validated as per united states food and drug administration (USFDA) guidelines by the study of accuracy, precision, linearity, range, selectivity, the lower limit of quantitation, extraction recovery studies and stability studies for determining oxalate content in the urine of human volunteers. As relative standard deviations of oxalate content estimated are less than 5 percent, the method can be claimed accurate, precise, sensitive and selective for determining oxalate content in the urine of human volunteers.Conclusion: The results are satisfactory, proving the effectiveness of the method for analysis of oxalate content from other biological fluids with few optimizations

ASSESSMENT OF ANTIMICROBIAL EFFICACY OF KOHL/KAJAL PREPARED BY DIFFERENT INDIAN METHODS AGAINST SELECTED MICROBIAL STRAINS

Objective: To prepare and evaluate different types of Kajal formulations and evaluation of its antimicrobial activity along with preliminary verification of the content responsible for the said effect. Methods: We have prepared kajal formulations by use of different metal plates, marble tile, ghee and Aloe vera mucilage and tried to verify the antimicrobial effect attributed to the formulation by these substances. Results: Carbon soot obtained from the use of copper plate showed more antimicrobial potential against Staphylococcus aureus, Pseudomonas aeruginosa and E. coli, with zones of inhibition 18±0.235 mm, 17±0.124 mm and 19±0.528 mm respectively. Also this formulation at different concentrations when compared with Ciprofloxacin exhibited promising results. Moreover, this formulation when used with Ciprofloxacin at a concentration of (50:50) revealed a synergistic effect against the clinically resistant strains of P. aeruginosa, with zone of inhibition 22±0.578 mm and 20±0.987 mm at a concentration of 10 and 5 µg ml-1 respectively, whereas, Ciprofloxacin exhibited zone of inhibition of 26±0.457 mm and 24±0.751 mm at the similar concentrations. To assess the effectiveness of Aloe vera we used marbles tiles for collection of carbon soot. The zones of inhibition observed for Kohl formulations prepared by using carbon soot collected from marble tiles impregnated with Aloe vera mucilage exhibited less antimicrobial activity than that of copper soot against the selected microbial strains. Conclusion: All the prepared kajal formulations exhibited antimicrobial activity. Aloe vera and copper soot is responsible for significant antimicrobial activity and when combined with Ciprofloxacin it showed synergistic activity against the clinically resistant strains of P. aeruginosa

Implementation of Decoy Deception based Detection System for Ransomware Attack

Ransomware poses a dangerous threat to  cybersecurity. Data as well as rights owned by the user are  adversely impacted. The situation has become considerably  more critical as a result of the emergence of new ransomware  varieties and Ransomware-as-a-Service. In this paper, we  presented a novel deception-based and behaviour-based  method for real-time ransomware detection. In order to avoid  any loss before ransomware is discovered, we build pretend  files and directories for nefarious behaviours. We conducted a  pilot study using Locky, and the results demonstrate the  effectiveness of our strategy with little system resource usage  and geographical cost.&nbsp

Role Based Secure Data Access Control for Cost Optimized Cloud Storage Using Data Fragmentation While Maintaining Data Confidentiality

The paper proposes a role-based secure data access control framework for cost-optimized cloud storage, addressing the challenge of maintaining data security, privacy, integrity, and availability at lower cost. The proposed framework incorporates a secure authenticity scheme to protect data during storage or transfer over the cloud. The framework leverages storage cost optimization by compressing high-resolution images and fragmenting them into multiple encrypted chunks using the owner's private key. The proposed approach offers two layers of security, ensuring that only authorized users can decrypt and reconstruct data into its original format. The implementation results depicts that the proposed scheme outperforms existing systems in various aspects, making it a reliable solution for cloud service providers to enhance data security while reducing storage costs

In vitro florigenesis: an efficient regeneration system avoiding time consuming vegetative phase in popular Indian soybean variety JS-335

458-466Soybean (Glycine max L.) is a major legume crop cultivated principally as protein rich bean. Improving quality and yield received considerable attention from researchers. Here, we explored molecular approaches to improve soybean quality and yield. In spite of recalcitrance, soybean plants successfully regenerated through complex and time consuming in vitro regeneration protocols via organogenesis and/or somatic embryogenesis and being used for transgenic development. Transformation efficiency is highly dependent on the regeneration as not all the cells transformed lead to the recovery of viable plant regeneration. Consequently, efficient in vitro regeneration found to be directly associated with the recovery of transformants. In the present study, we standardized the in vitro florigenesis using cotyledonary node with axillary bud as explant of soybean variety JS-335. Flower buds were directly induced from proximal end of the explant on Murashige-Skoog (MS) medium augmented with thidiazuron (TDZ) and α naphthalene acetic acid (NAA). TDZ proved a potential growth regulator to induce in vitro florigenesis. As a result of in vitro fertilization, pods were developed from flowers and matured within 40-45 days on hormone-free medium. Pods and seed set under in vitro conditions resemble pods and seeds produced under in vivo conditions. This pathway of in vitro florigenesis showed great potential for successful induction of in vitro flowering, which in turn can be explored in producing transgenic soybean seeds in popular Indian soybean genotype without escaping transgene

Soil and Herbage Heavy Metal/ Trace Element Variability and Relationships at Farm and Regional Level.

End of Project ReportVariability of heavy metal trace/element amounts in soil and herbage from 3 farms encompassing 26.5, 33.6 and 21.6 ha and in a 230 km2 surrounding area has been estimated. Variability was least in the farm with the greatest soil physical uniformity. It increased in line with increasing sampling area. In soil, variability was least (CV 10%) for sesquioxide metals, iron and aluminium, and elements chromium and nickel. It was highest where there was evidence of geochemical contamination with cadmium and selenium. In general, variability was higher (>20% CV) for extractable components including copper, zinc and manganese. Regression analysis of elements in soil showed up a number of useful associations particularly for the volatiles selenium, sulphate sulphur, mercury, cobalt, vanadium and fluorine with soil organic matter. Associations were also found between fluorine, vanadium and chromium. • Elements in herbage exhibited two types of associations (i) those reckoned to be within the plant and confined mostly to major elements and (ii) those involving metals associated primarily with soil and indicative of the extent to which herbage was contaminated by soil. • Elements that showed lower variability in soil exhibited large variability in herbage. These included iron, aluminium, chromium, vanadium and fluorine, where the cause was attributed to contamination of herbage by soil. It was suggested that iron could be used almost equally as well as titanium as an indicator of the degree to which herbage has been contaminated by soil. • Long term monitoring of herbage for selenium content highlighted the unreliability of relying on a single determination to characterise the selenium status of pasture

In vitro florigenesis: an efficient regeneration system avoiding time consuming vegetative phase in popular Indian soybean variety JS-335

Soybean (Glycine max L.) is a major legume crop cultivated principally as protein rich bean. Improving quality and yield received considerable attention from researchers. Here, we explored molecular approaches to improve soybean quality and yield. In spite of recalcitrance, soybean plants successfully regenerated through complex and time consuming in vitro regeneration protocols via organogenesis and/or somatic embryogenesis and being used for transgenic development. Transformation efficiency is highly dependent on the regeneration as not all the cells transformed lead to the recovery of viable plant regeneration. Consequently, efficient in vitro regeneration found to be directly associated with the recovery of transformants. In the present study, we standardized the in vitro florigenesis using cotyledonary node with axillary bud as explant of soybean variety JS-335. Flower buds were directly induced from proximal end of the explant on Murashige-Skoog (MS) medium augmented with thidiazuron (TDZ) and α naphthalene acetic acid (NAA). TDZ proved a potential growth regulator to induce in vitro florigenesis. As a result of in vitro fertilization, pods were developed from flowers and matured within 40-45 days on hormone-free medium. Pods and seed set under in vitro conditions resemble pods and seeds produced under in vivo conditions. This pathway of in vitro florigenesis showed great potential for successful induction of in vitro flowering, which in turn can be explored in producing transgenic soybean seeds in popular Indian soybean genotype without escaping transgene

SCN5A mutations in 442 neonates and children: genotype-phenotype correlation and identification of higher-risk subgroups.

Aims To clarify the clinical characteristics and outcomes of children with SCN5A-mediated disease and to improve their risk stratification. Methods and results A multicentre, international, retrospective cohort study was conducted in 25 tertiary hospitals in 13 countries between 1990 and 2015. All patients ≤16 years of age diagnosed with a genetically confirmed SCN5A mutation were included in the analysis. There was no restriction made based on their clinical diagnosis. A total of 442 children {55.7% boys, 40.3% probands, median age: 8.0 [interquartile range (IQR) 9.5] years} from 350 families were included; 67.9% were asymptomatic at diagnosis. Four main phenotypes were identified: isolated progressive cardiac conduction disorders (25.6%), overlap phenotype (15.6%), isolated long QT syndrome type 3 (10.6%), and isolated Brugada syndrome type 1 (1.8%); 44.3% had a negative electrocardiogram phenotype. During a median follow-up of 5.9 (IQR 5.9) years, 272 cardiac events (CEs) occurred in 139 (31.5%) patients. Patients whose mutation localized in the C-terminus had a lower risk. Compound genotype, both gain- and loss-of-function SCN5A mutation, age ≤1 year at diagnosis in probands and age ≤1 year at diagnosis in non-probands were independent predictors of CE. Conclusion In this large paediatric cohort of SCN5A mutation-positive subjects, cardiac conduction disorders were the most prevalent phenotype; CEs occurred in about one-third of genotype-positive children, and several independent risk factors were identified, including age ≤1 year at diagnosis, compound mutation, and mutation with both gain- and loss-of-function