'The sight of sound': Gebärdensprachdolmetschen auf der lautsprachlichen Theaterbühne am Beispiel einer gedolmetschten Aufführung von 'My fair lady' am Hans Otto Theater Potsdam

Während die Verdolmetschung lautsprachlicher Theateraufführungen in die Gebärdensprache in vielen Ländern selbstverständlich und regelmäßig angeboten wird, handelt es sich in Deutschland dabei noch um Einzelerscheinungen. Eine Ausnahme stellt das Hans Otto Theater Potsdam dar, das seit 1996 regelmäßig gedolmetschte Aufführungen anbietet und dabei die Methode des Shadow Interpreting nutzt. Am Beispiel einer gedolmetschten Aufführung von „My Fair Lady“ am Hans Otto Theater werden in dieser Arbeit folgende Aspekte der gebärdensprachlichen Verdolmetschung von Theateraufführungen untersucht: Stückauswahl, Dolmetscheranzahl und Rollenverteilung, Auswahl und Einführung von Namensgebärden der Figuren, Positionierung der Dolmetscher, Rollendarstellung und Rollenwechsel, Übertragung der akustischen Ebene des Aufführungstextes (linguistische und paralinguistische Informationen, Musik, Geräusche), äußere Erscheinung der Dolmetscher, Beleuchtung der Dolmetscher und die Inkorporation der Dolmetscher in die Aufgabe

Calculating confidence intervals for impact numbers

BACKGROUND: Standard effect measures such as risk difference and attributable risk are frequently used in epidemiological studies and public health research to describe the effect of exposures. Recently, so-called impact numbers have been proposed, which express the population impact of exposures in form of specific person or case numbers. To describe estimation uncertainty, it is necessary to calculate confidence intervals for these new effect measures. In this paper, we present methods to calculate confidence intervals for the new impact numbers in the situation of cohort studies. METHODS: Beside the exposure impact number (EIN), which is equivalent to the well-known number needed to treat (NNT), two other impact numbers are considered: the case impact number (CIN) and the exposed cases impact number (ECIN), which describe the number of cases (CIN) and the number of exposed cases (ECIN) with an outcome among whom one case is attributable to the exposure. The CIN and ECIN represent reciprocals of the population attributable risk (PAR) and the attributable fraction among the exposed (AF(e)), respectively. Thus, confidence intervals for these impact numbers can be calculated by inverting and exchanging the confidence limits of the PAR and AF(e). EXAMPLES: We considered a British and a Japanese cohort study that investigated the association between smoking and death from coronary heart disease (CHD) and between smoking and stroke, respectively. We used the reported death and disease rates and calculated impact numbers with corresponding 95% confidence intervals. In the British study, the CIN was 6.46, i.e. on average, of any 6 to 7 persons who died of CHD, one case was attributable to smoking with corresponding 95% confidence interval of [3.84, 20.36]. For the exposed cases, the results of ECIN = 2.64 with 95% confidence interval [1.76, 5.29] were obtained. In the Japanese study, the CIN was 6.67, i.e. on average, of the 6 to 7 persons who had a stroke, one case was attributable to smoking with corresponding 95% confidence interval of [3.80, 27.27]. For the exposed cases, the results of ECIN = 4.89 with 95% confidence interval of [2.86, 16.67] were obtained. CONCLUSION: The consideration of impact numbers in epidemiological analyses provides additional information and helps the interpretation of study results, e.g. in public health research. In practical applications, it is necessary to describe estimation uncertainty. We have shown that the calculation of confidence intervals for the new impact numbers is possible by means of known methods for attributable risk measures. Therefore, estimated impact numbers should always be complemented by appropriate confidence intervals

'The sight of sound': Gebärdensprachdolmetschen auf der lautsprachlichen Theaterbühne am Beispiel einer gedolmetschten Aufführung von 'My fair lady' am Hans Otto Theater Potsdam

Während die Verdolmetschung lautsprachlicher Theateraufführungen in die Gebärdensprache in vielen Ländern selbstverständlich und regelmäßig angeboten wird, handelt es sich in Deutschland dabei noch um Einzelerscheinungen. Eine Ausnahme stellt das Hans Otto Theater Potsdam dar, das seit 1996 regelmäßig gedolmetschte Aufführungen anbietet und dabei die Methode des Shadow Interpreting nutzt. Am Beispiel einer gedolmetschten Aufführung von „My Fair Lady“ am Hans Otto Theater werden in dieser Arbeit folgende Aspekte der gebärdensprachlichen Verdolmetschung von Theateraufführungen untersucht: Stückauswahl, Dolmetscheranzahl und Rollenverteilung, Auswahl und Einführung von Namensgebärden der Figuren, Positionierung der Dolmetscher, Rollendarstellung und Rollenwechsel, Übertragung der akustischen Ebene des Aufführungstextes (linguistische und paralinguistische Informationen, Musik, Geräusche), äußere Erscheinung der Dolmetscher, Beleuchtung der Dolmetscher und die Inkorporation der Dolmetscher in die Aufgabe

'The sight of sound': Gebärdensprachdolmetschen auf der lautsprachlichen Theaterbühne am Beispiel einer gedolmetschten Aufführung von 'My fair lady' am Hans Otto Theater Potsdam

Während die Verdolmetschung lautsprachlicher Theateraufführungen in die Gebärdensprache in vielen Ländern selbstverständlich und regelmäßig angeboten wird, handelt es sich in Deutschland dabei noch um Einzelerscheinungen. Eine Ausnahme stellt das Hans Otto Theater Potsdam dar, das seit 1996 regelmäßig gedolmetschte Aufführungen anbietet und dabei die Methode des Shadow Interpreting nutzt. Am Beispiel einer gedolmetschten Aufführung von „My Fair Lady“ am Hans Otto Theater werden in dieser Arbeit folgende Aspekte der gebärdensprachlichen Verdolmetschung von Theateraufführungen untersucht: Stückauswahl, Dolmetscheranzahl und Rollenverteilung, Auswahl und Einführung von Namensgebärden der Figuren, Positionierung der Dolmetscher, Rollendarstellung und Rollenwechsel, Übertragung der akustischen Ebene des Aufführungstextes (linguistische und paralinguistische Informationen, Musik, Geräusche), äußere Erscheinung der Dolmetscher, Beleuchtung der Dolmetscher und die Inkorporation der Dolmetscher in die Aufgabe

Calculation of NNTs in RCTs with time-to-event outcomes: A literature review

Abstract Background The number needed to treat (NNT) is a well-known effect measure for reporting the results of clinical trials. In the case of time-to-event outcomes, the calculation of NNTs is more difficult than in the case of binary data. The frequency of using NNTs to report results of randomised controlled trials (RCT) investigating time-to-event outcomes and the adequacy of the applied calculation methods are unknown. Methods We searched in PubMed for RCTs with parallel group design and individual randomisation, published in four frequently cited journals between 2003 and 2005. We evaluated the type of outcome, the frequency of reporting NNTs with corresponding confidence intervals, and assessed the adequacy of the methods used to calculate NNTs in the case of time-to-event outcomes. Results The search resulted in 734 eligible RCTs. Of these, 373 RCTs investigated time-to-event outcomes and 361 analyzed binary data. In total, 62 articles reported NNTs (34 articles with time-to-event outcomes, 28 articles with binary outcomes). Of the 34 articles reporting NNTs derived from time-to-event outcomes, only 17 applied an appropriate calculation method. Of the 62 articles reporting NNTs, only 21 articles presented corresponding confidence intervals. Conclusion The NNT is used as effect measure to present the results from RCTs with binary and time-to-event outcomes in the current medical literature. In the case of time-to-event data incorrect methods were frequently applied. Confidence intervals for NNTs were given in one third of the NNT reporting articles only. In summary, there is much room for improvement in the application of NNTs to present results of RCTs, especially where the outcome is time to an event.</p

Maturation-dependent differences in the re-innervation of the denervated dentate gyrus by sprouting associational and commissural mossy cell axons in organotypic tissue cultures of entorhinal cortex and hippocampus

Sprouting of surviving axons is one of the major reorganization mechanisms of the injured brain contributing to a partial restoration of function. Of note, sprouting is maturation as well as age-dependent and strong in juvenile brains, moderate in adult and weak in aged brains. We have established a model system of complex organotypic tissue cultures to study sprouting in the dentate gyrus following entorhinal denervation. Entorhinal denervation performed after 2 weeks postnatally resulted in a robust, rapid, and very extensive sprouting response of commissural/associational fibers, which could be visualized using calretinin as an axonal marker. In the present study, we analyzed the effect of maturation on this form of sprouting and compared cultures denervated at 2 weeks postnatally with cultures denervated at 4 weeks postnatally. Calretinin immunofluorescence labeling as well as time-lapse imaging of virally-labeled (AAV2- hSyn1-GFP) commissural axons was employed to study the sprouting response in aged cultures. Compared to the young cultures commissural/associational sprouting was attenuated and showed a pattern similar to the one following entorhinal denervation in adult animals in vivo. We conclude that a maturation-dependent attenuation of sprouting occurs also in vitro, which now offers the chance to study, understand and influence maturation-dependent differences in brain repair in these culture preparations

Re-innervation of the denervated dentate gyrus by sprouting associational and commissural mossy cell axons in organotypic tissue cultures of entorhinal cortex and hippocampus

Collateral sprouting of surviving axons contributes to the synaptic reorganization after brain injury. To study this clinically relevant phenomenon, we used complex organotypic tissue cultures of mouse entorhinal cortex (EC) and hippocampus (H). Single EC-H cultures were generated to analyze associational sprouting, and double EC-H cultures were used to evaluate commissural sprouting of mossy cells in the dentate gyrus (DG) following entorhinal denervation. Entorhinal denervation (transection of the perforant path) was performed at 14 days in vitro (DIV) and associational/commissural sprouting was assessed at 28 DIV. First, associational sprouting was studied in genetically hybrid EC-H cultures of beta-actin-GFPtg and wild-type mice. Using calretinin as a marker, associational axons were found to re-innervate almost the entire entorhinal target zone. Denervation experiments performed with EC-H cultures of Thy1-YFPtg mice, in which mossy cells are YFP-positive, confirmed that the overwhelming majority of sprouting associational calretinin-positive axons are mossy cell axons. Second, we analyzed associational/commissural sprouting by combining wild-type EC-H cultures with calretinin-deficient EC-H cultures. In these cultures, only wild-type mossy cells contain calretinin, and associational and commissural mossy cell collaterals can be distinguished using calretinin as a marker. Nearly the entire DG entorhinal target zone was re-innervated by sprouting of associational and commissural mossy cell axons. Finally, viral labeling of newly formed associational/commissural axons revealed a rapid post-lesional sprouting response. These findings demonstrate extensive and rapid re-innervation of the denervated DG outer molecular layer by associational and commissural mossy cell axons, similar to what has been reported to occur in juvenile rodent DG in vivo

ATP Release from Human Airway Epithelial Cells Exposed to Staphylococcus aureus Alpha-Toxin

Airway epithelial cells reduce cytosolic ATP content in response to treatment with S. aureus alpha-toxin (hemolysin A, Hla). This study was undertaken to investigate whether this is due to attenuated ATP generation or to release of ATP from the cytosol and extracellular ATP degradation by ecto-enzymes. Exposure of cells to rHla did result in mitochondrial calcium uptake and a moderate decline in mitochondrial membrane potential, indicating that ATP regeneration may have been attenuated. In addition, ATP may have left the cells through transmembrane pores formed by the toxin or through endogenous release channels (e.g., pannexins) activated by cellular stress imposed on the cells by toxin exposure. Exposure of cells to an alpha-toxin mutant (H35L), which attaches to the host cell membrane but does not form transmembrane pores, did not induce ATP release from the cells. The Hla-mediated ATP-release was completely blocked by IB201, a cyclodextrin-inhibitor of the alpha-toxin pore, but was not at all affected by inhibitors of pannexin channels. These results indicate that, while exposure of cells to rHla may somewhat reduce ATP production and cellular ATP content, a portion of the remaining ATP is released to the extracellular space and degraded by ecto-enzymes. The release of ATP from the cells may occur directly through the transmembrane pores formed by alpha-toxin

Crossed entorhino-dentate projections form and terminate with correct layer-specificity in organotypic slice cultures of the mouse hippocampus

The entorhino-dentate projection, i.e., the perforant pathway, terminates in a highly ordered and laminated fashion in the rodent dentate gyrus (DG): fibers arising from the medial entorhinal cortex (MEC) terminate in the middle molecular layer, whereas fibers arising from the lateral entorhinal cortex (LEC) terminate in the outer molecular layer of the DG. In rats and rabbits, a crossed entorhino-dentate projection exists, which originates from the entorhinal cortex (EC) and terminates in the contralateral DG. In contrast, in mice, such a crossed projection is reportedly absent. Using single and double mouse organotypic entorhino-hippocampal slice cultures, we studied the ipsi- and crossed entorhino-dentate projections. Viral tracing revealed that entorhino-dentate projections terminate with a high degree of lamina-specificity in single as well as in double cultures. Furthermore, in double cultures, entorhinal axons arising from one slice freely intermingled with entorhinal axons originating from the other slice. In single as well as in double cultures, entorhinal axons exhibited a correct topographical projection to the DG: medial entorhinal axons terminated in the middle and lateral entorhinal axons terminated in the outer molecular layer. Finally, entorhinal neurons were virally transduced with Channelrhodopsin2-YFP and stimulated with light, revealing functional connections between the EC and dentate granule cells. We conclude from our findings that entorhino-dentate projections form bilaterally in the mouse hippocampus in vitro and that the mouse DG provides a permissive environment for crossed entorhinal fibers