Exobiology and the search for biological signatures on Mars

In preparation for a Mars Rover/Sample return mission, the mission goals and objectives must be identified. One of the most important objectives must address exobiology and the question of the possibility of the origin and evolution of life on Mars. In particular, key signatures or bio-markers of a possible extinct Martian biota must be defined. To that end geographic locations (sites) that are likely to contain traces of past life must also be identified. Sites and experiments are being defined in support of a Mars rover sample return mission. In addition, analyses based on computer models of abiotic processes of CO2 loss from Mars suggest that the CO2 from the atmosphere may have precipitated as carbonates and be buried within the Martian regolith. The carbon cycle of perennially frozen lakes in the dry valley of Antarctica are currently being investigated. These lakes were purported to be a model system for the ancient Martian lakes. By understanding the dynamic balance between the abiotic vs. biotic cycling of carbon within this system, information is gathered which will enable the interpretation of data obtained by a Mars rover with respect to possible carbonate deposits and the processing of carbon by biological systems. These ancient carbonate deposits, and other sedimentary units would contain traces of biological signatures that would hold the key to understanding the origin and evolution of life on Mars, as well as Earth

Effects of Flight on Gene Expression and Aging in the Honey Bee Brain and Flight Muscle

Honey bees move through a series of in-hive tasks (e.g., “nursing”) to outside tasks (e.g., “foraging”) that are coincident with physiological changes and higher levels of metabolic activity. Social context can cause worker bees to speed up or slow down this process, and foragers may revert back to their earlier in-hive tasks accompanied by reversion to earlier physiological states. To investigate the effects of flight, behavioral state and age on gene expression, we used whole-genome microarrays and real-time PCR. Brain tissue and flight muscle exhibited different patterns of expression during behavioral transitions, with expression patterns in the brain reflecting both age and behavior, and expression patterns in flight muscle being primarily determined by age. Our data suggest that the transition from behaviors requiring little to no flight (nursing) to those requiring prolonged flight bouts (foraging), rather than the amount of previous flight per se, has a major effect on gene expression. Following behavioral reversion there was a partial reversion in gene expression but some aspects of forager expression patterns, such as those for genes involved in immune function, remained. Combined with our real-time PCR data, these data suggest an epigenetic control and energy balance role in honey bee functional senescence

Carbon budget and national gross domestic product in the framework of the Paris Climate Agreement

In 2015 an unprecedented effort was made in Paris by the countries adhering to the United Nations Framework Convention on Climate Change to decrease the CO2 emissions due to the close relationships of greenhouse gases with global warming. Under the previous Kyoto Protocol, only advanced countries were committed to reduce greenhouse emissions while under the Paris Climate Agreement all countries were committed to fight against global warming. The urgency of real action has been prompted by extreme events like bushfires, heatwaves, and the ongoing pandemic. Given the strong commitments, it looks interesting, seen that all countries are involved, to verify if any sustainability pattern is evident. Our approach is encouraging, as the downward emission trend shows a high increase in sustainability between 2027 and 2037. Without exacerbating the climate discussion, we used the national gross domestic product (hereafter: GDP) as environmental indicator to propose for the first time an allometric ranking of countries that need to change drastically their energy policy to meet their climate commitments. Any sustainability downturn in one country, especially if advanced, might rationally bring concern about the actual prospects of other countries which are all committed to the Paris Climate Agreement. But the departure from the annual allometric model GDP−CO2 may be much greater than can be accounted for by statistical expectations, as for Ukraine, Uzbekistan and Turkmenistan that are entering a sustainable condition where their CO2 emissions will be lower than they would have been without the Paris Climate Agreement

Alternative Cropping Systems with Self Reseeding Annual Legumes in a Mediterranean Environment

Self-reseeding winter annual legumes (subclover and snail medic) have the potential to induce a significant shift towards a less energy-intensive and more environmentally friendly management in the modern and specialized cereal cropping systems. Nevertheless their use is practically unknown in mixed stands with winter cereals, where they can contribute to supply nitrogen and to reduce herbicides input. We conceived an alternative cropping system where an annual legume performs, respectively, as living mulch in a winter cereal (winter wheat), as a cover crop after reseeding and as dry mulch for the succeeding summer cereal (corn). Trials carried out at the Tuscia University (Central Italy) in the period 1995-1997 showed that almost all the tested legumes cultivars were able both to grow sufficiently as a living mulch in the wheat and to provide a good re-establishment and an abundant mulch after the wheat harvest for the next corn. Trifolium yanninicum cv Trikkala and Trifolium subterraneum cvs Karridale and Mount Barker ranked first in reseeding capacity (up to 400 seedlings m-2) and mulch production (up to 5 t ha-1). No difference in grain yield between wheat with a living mulch and wheat in pure stand was found when legumes, such as Trifolium yanninicum cvs Trikkala and Larisa, Trifolium brachycalycinum cv Clare, Trifolium subterraneum cv Nungarin and Medicago scutellata cv Kelson, were grown in binary mixture with wheat. The aboveground biomass production of the succeeding irrigated corn crop was largely dependent on the amount of legume dry mulch left upon the ground

Legumes for Organic Cropping Systems

A field experiment which focuses on the yield performance of both durum wheat and sunflower included in a twoyear rotation between wheat/subterranean clover and sunflower was carried out in an organic farm in Central Italy. Three factors were included in the experiment according to a factorial design: a) two different previous crop sequences (four years of alfalfa, four years of annual crops); b) three fertilization levels (without and with animal manure incorporated at 15 or 40 cm depth); c) three durum wheat genotypes (Appulo, Arcangelo and Daunia cvs). The results obtained show that a previous alfalfa meadow allows the succeeding crops (wheat and sunflower) to achieve an appropriate and stable grain yield (about 4 and 3 t ha-1, respectively) even without organic manure for two consecutive cycles. When the durum wheat follows an annual crop sequence, grain yield appears unstable and the different levels of organic fertilizer determine a negative effect in the first cycle and a positive effect in the second cycle. The reseeding capability of sunflower was reduced by animal manure treatments and, consequently, the subclover biomass and its nitrogen content were generally lower in fertilized plots. Anyway, subclover biomass, which was ploughed in after reseeding as a green manure for sunflower, proved to be effective in stabilizing sunflower grain yield, especially in plots without any organic fertilization

Muscle biopsy and cell cultures: potential diagnostic tools in hereditary skeletal muscle channelopathies.

Hereditary muscle channelopathies are caused by dominant mutations in the genes encoding for subunits of muscle voltage- gated ion channels. Point mutations on the human skeletal muscle Na+ channel (Nav1.4) give rise to hyperkalemic periodic paralysis, potassium aggravated myotonia, paramyotonia congenita and hypokalemic periodic paralysis type 2. Point mutations on the human skeletal muscle Ca2+ channel give rise to hypokalemic periodic paralysis and malignant hyperthermia. Point mutations in the human skeletal chloride channel ClC-1 give rise to myotonia congenita. Point mutations in the inwardly rectifying K+ channel Kir2.1 give rise to a syndrome characterized by periodic paralysis, severe cardiac arrhythmias and skeletal alterations (Andersen's syndrome). Involvement of the same ion channel can thus give rise to different phenotypes. In addition, the same mutation can lead to different phenotypes or similar phenotypes can be caused by different mutations on the same or on different channel subtypes. Bearing in mind, the complexity of this field, the growing number of potential channelopathies (such as the myotonic dystrophies), and the time and cost of the genetic procedures, before a biomolecular approach is addressed, it is mandatory to apply strict diagnostic protocols to screen the patients. In this study we propose a protocol to be applied in the diagnosis of the hereditary muscle channelopathies and we demonstrate that muscle biopsy studies and muscle cell cultures may significantly contribute towards the correct diagnosis of the channel involved. DNAbased diagnosis is now a reality for many of the channelopathies. This has obvious genetic counselling, prognostic and therapeutic implications

Mars rover sample return: An exobiology science scenario

A mission designed to collect and return samples from Mars will provide information regarding its composition, history, and evolution. At the same time, a sample return mission generates a technical challenge. Sophisticated, semi-autonomous, robotic spacecraft systems must be developed in order to carry out complex operations at the surface of a very distant planet. An interdisciplinary effort was conducted to consider how much a Mars mission can be realistically structured to maximize the planetary science return. The focus was to concentrate on a particular set of scientific objectives (exobiology), to determine the instrumentation and analyses required to search for biological signatures, and to evaluate what analyses and decision making can be effectively performed by the rover in order to minimize the overhead of constant communication between Mars and the Earth. Investigations were also begun in the area of machine vision to determine whether layered sedimentary structures can be recognized autonomously, and preliminary results are encouraging

Habitat Patch Diversity Evaluation for Sustainability: a Case Study of a Rural Area in Central Italy

Landscape analysis is regarded as a new tool for monitoring and judging land use patterns in terms of sustainability of human activity systems at local level. A case study of evaluation for sustainability based on habitat patch diversity in an ecoregion of Central Italy is presented. In this region, ongoing land use patterns reflect both historical adaptation to local environmental constraints and positive, social-oriented management. More protective land use patterns are mostly widespread in fragile physiographic conditions like those of the mountain areas, where woodland, shrub, and grassland patches are larger and cover more than 90% of the land. This situation is regarded as a positive outcome of the traditional public ownership regime, because public lands amount to more than 70% in the mountain areas. The hilly areas, where public property drops to 28%, presents landscape metrics showing a well balanced situation between agricultural land use and protective native woods and grasslands, which provides a fine-grained and harmonious Mediterranean landscape. In the low-land areas, with anthropic pressure and more favourable conditions for crop productivity, there is much more agricultural land, even if some mitigation in terms of biodiversity maintenance is offered by the presence of hedgerow ecotones. In these areas, landscape analysis is not able to supply meaningful information about cropping system design and practices which can maintain a sustainable level of soil fertility and quality of natural resources and processes, and further analysis at cropping system level should be carried out

Mars Rover Sample Return: A sample collection and analysis strategy for exobiology

For reasons defined elsewhere it is reasonable to search for biological signatures, both chemical and morphological, of extinct life on Mars. Life on Earth requries the presence of liquid water, therefore, it is important to explore sites on Mars where standing bodies of water may have once existed. Outcrops of layered deposits within the Valles Marineris appear to be ancient lake beds. Because the outcrops are well exposed, relatively shallow core samples would be very informative. The most important biological signature to detect would be organics, microfossils, or larger stromato-like structures, although the presence of cherts, carbonates, clays, and shales would be significant. In spite of the limitations of current robotics and pattern recognition, and the limitations of rover power, computation, Earth communication bandwidth, and time delays, a partial scenario was developed to implement such a scientific investigation. The rover instrumentation and the procedures and decisions and IR spectrometer are described in detail. Preliminary results from a collaborative effort are described, which indicate the rover will be able to autonomously detect stratification, and hence will ease the interpretation burden and lead to greater scientific productivity during the rover's lifetime

Ribonuclear inclusions as biomarker of myotonic dystrophy type 2, even in improperly frozen or defrozen skeletal muscle biopsies

Myotonic dystrophy type 2 (DM2) is a dominantly inherited disorder caused by a CCTG repeat expansion in intron 1 of ZNF9 gene. The size and the somatic instability of DM2 expansion complicate the molecular diagnosis of DM2. In situ hybridization represents a rapid and sensitive method to obtain a definitive diagnosis in few hours, since it allows the direct visualization of the mutant mRNA foci on skeletal muscle sections. This approach makes the muscle biopsy an important tool for definitive diagnosis of DM2. Consequently, a rapid freezing at ultra cold temperature and a good storage of muscle specimens are essential to avoid morphologic alterations and nucleic acids degradation. However incorrect freezing or thawing may accidentally occur. In this work we report that fluorescence in situ hybridization may be applied on improperly frozen or inappropriately stored muscle biopsies since foci of mutant mRNA are well preserved and can still be detected in muscle sections no more useful for histopathological evaluation