Enfermedades infecciosas gastrointestinales

El presente trabajo fue realizado en el Servicio de Internación Pediátrica NºII del Hospital Dr. Humberto Notti, ubicado en Banderas de los Andes 2603, en el departamento de Guaymallén. Está orientado a investigar la relación que existe entre la aparición de infecciones gastrointestinales y el origen social, tomando como muestra a los pacientes menores de 5 años internados en dicho servicio comprendidos entre los meses de septiembre, octubre y noviembre de 2013. El objetivo de esta investigación es encontrar y determinar la relación que existe entre las variables mencionadas anteriormente, como así también determinar y valorar los conocimientos previos que poseen los familiares del paciente, acerca de factores importantes que pueden influir como el lavado de manos, manipulación de alimentos y nutrición; es decir describir el aspecto socio cultural de los mismos.Fil: Lama, Janet M.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Escuela de Enfermería..Fil: Mancilla, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Escuela de Enfermería..Fil: Castro, Romina V.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Escuela de Enfermería.

Comparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroups

Indexación: Scopus.Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes. Two marked genogroups were identified, as confirmed by phylogenetic and phylogenomic relationships to the LF-89 and EM-90 reference strains, as well as by assessments of genomic structures. Different structural configurations were found for the six identified copies of the ribosomal operon in the P. salmonis genome, indicating translocation throughout the genetic material. Chromosomal divergences in genomic localization and quantity of genetic cassettes were also found for the Dot/Icm type IVB secretion system. To determine divergences between core-genomes, additional pan-genome descriptions were compiled for the so-termed LF and EM genogroups. Open pan-genomes composed of 2,924 and 2,778 genes and core-genomes composed of 2,170 and 2,228 genes were respectively found for the LF and EM genogroups. The core-genomes were functionally annotated using the Gene Ontology, KEGG, and Virulence Factor databases, revealing the presence of several shared groups of genes related to basic function of intracellular survival and bacterial pathogenesis. Additionally, the specific pan-genomes for the LF and EM genogroups were defined, resulting in the identification of 148 and 273 exclusive proteins, respectively. Notably, specific virulence factors linked to adherence, colonization, invasion factors, and endotoxins were established. The obtained data suggest that these genes could be directly associated with inter-genogroup differences in pathogenesis and host-pathogen interactions, information that could be useful in designing novel strategies for diagnosing and controlling P. salmonis infection. © 2017 Nourdin-Galindo, Sánchez, Molina, Espinoza-Rojas, Oliver, Ruiz, Vargas-Chacoff, Cárcamo, Figueroa, Mancilla, Maracaja-Coutinho and Yañez.https://www.frontiersin.org/articles/10.3389/fcimb.2017.00459/ful

Effect of different digestates derived from anaerobic co-digestion of Olive Mill Solid Waste (OMSW) and various microalgae as fertilizers for the cultivation of ryegrass

Aims The aim of this work was to evaluate the fertilizing effect of three anaerobic co-digestates on the growth of the herbaceous plant Lolium rigidum. Methods Nine treatments, combining different nutritional solutions (organic and inorganic) and number of fertilizations (one or two) were evaluated. Organic nutritive solution: plants grown with different olive mill solid waste (OMSW) -microalgae co-digestates: 75% OMSW-25% Raphidocelis subcapitata, volatile solids (VS) basis (OMSW-Rs); 50% OMSW- 50% Chlamydomonas Reinhardtii, VS basis (OMSW-Chl); and 75% OMSW-25% Secenedesmus quadricauda, VS basis (OMSW-Sq). Inorganic nutritive solution (INS): plants grown with inorganic Hoagland nutrient solution at 50%. After 60 days of experimentation, biometric and nutritional characteristics and photosynthetic activity were measured. Results The results showed a favourable growth, development and nutritional quality of L. rigidum plants when digestates obtained from the anaerobic co-digestion of OMSW-microalgae are used as organic nutritional solutions as opposed to INS ones. The highest total biomass of L. rigidum was obtained with the treatments that involved two fertilizations. No inhibition due to excess nutrients was observed. A higher root/shoot ratio was achieved with the digestates of OMSW-Rs and OMSW-Ch as compared to that obtained with OMSW-Sq (F = 17.23 p ≤ 0.001). The nitrogen shoot biomass obtained after the organic treatments with the above-mentioned co-digestates was higher than that obtained after the inorganic treatment. Net photosynthesis rates did not present differences in the co-digestates treatments, being equal or superior to the INS treatments. Conclusions The use of the anaerobic co-digestates from OMSW-microalgae can be considered a viable and promising alternative to inorganic fertilization

Overriding Plate Thickness as a Controlling Factor for Trench Retreat Rates in Narrow Subduction Zones

Slab width is a significant factor in controlling subduction zone dynamics, particularly the retreat velocities, which tend to decrease with wider slabs. However, observations of natural narrow subduction zones reveal no correlation between slab width and trench velocities. This suggests that other factors may exert a greater influence. In this study, we employ 3D numerical subduction models to systematically assess the impact of slab width, strength of slab coupling to the lateral plate (LP), and overriding plate (OP) thickness on trench kinematics and geometry. Our models focus on narrow slabs (400–1,200 km), and the results demonstrate that, in the case of narrow subduction zones, the slab width has little effect on trench migration rates and the viscous coupling at the lateral slab edge is only important for very narrow subduction zones (≤800 km). Conversely, the OP thickness emerges as a crucial factor, with increasing plate thickness leading to a strong decrease in trench velocities. These findings provide an explanation for the observed trench velocities in natural narrow subduction zones, where an inverse relationship with OP thickness is evident. Furthermore, our study reveals that not only slab width, but also the OP thickness and the slab coupling to the LP, significantly influence trench geometry. Strong lateral coupling promotes the formation of concave trench geometries, while thick overriding plates favor the development of “w”‐shaped geometries. Overall, a comprehensive understanding of subduction processes necessitates considering the interplay between slab width, OP thickness, and slab coupling to the LP

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

The drug abuse known to occur during growth of animals intended for food production, because of their use as either a prophylactic or therapeutic treatment, promotes the emergence of bacterial drug resistance. It has been reported that at least 25% of the foodborne isolates show drug resistance to one or more classes of antimicrobials (FAO 2018). There are diverse mechanisms that promote drug resistance. It is known that the use of sub-therapeutic doses of antibiotics in animals intended for food production promotes mutations of some chromosomal genes such as gyrA-parC and mphA, which are responsible for quinolone and azithromycin resistance, respectively. Also, the horizontal transfer of resistance genes as groups (“cassettes”) or plasmids makes the spread of resistance to different bacterial genera possible, among which there could be pathogens. The World Health Organization considers the emergence of multidrug-resistant pathogenic bacteria as a health problem, since the illnesses caused by them complicate the treatment and increase the morbidity and mortality rates. The complication in the illness treatment caused by a multidrug-resistant pathogen causes economic losses to patients for the payment of long stays in hospitals and also causes economic losses to companies due to the absenteeism of their workers

Blast Wave Characteristics and TNT Equivalent of Improvised Explosive Device at Small scaled Distances

A significant number of airblast test have been carried out with the purpose to characterise and analyse the properties of improvised explosive device (IED) with non-conventional explosives in terms of knowing the effects on people and/or structures. Small devices with 1.5 kg of explosive, initiated with a detonating cord have been studied. Seven different mixtures have been tested with two types of ammonium nitrate AN (technical and fertilizer) in different forms like prills or powder. In some cases, the ammonium nitrate has been mixed with fuel oil while in others, it has been mixed with aluminum. The TNT equivalent based on pressure, impulse, arrival time, positive phase duration and shock front velocity have been calculated and analysed for each mixture. Comparing the field test data obtained with respect to the representation of the UFC 3-340-02 values, it can be seen that the parameters measured are consistent. The IEDs with fertilizer ammonium nitrate do not detonate with the present charge conditions so the shockwave generated is only due to the detonating cord. When using the technical ammonium nitrate, ANFO can partially detonate and generate a potentially dangerous shockwave. Finally, the IED with AN and aluminum produces a TNT equivalent close to one when the technical AN is used