THE RELATIONSHIP OF PHYSICAL ACTIVITY WITH THE QUALITY OF HEALTH OF ADOLESCENTS IN SELATAN MINAHASA DISTRICT

AbstrakSaat ini kualitas hidup menjadi salah satu kriteria yang digunakan untuk mengevaluasi intervensi pelayanan kesehatan. Remaja menjadi salah satu penentu dalam meningkatkan kualitas hidup masyarakat. Kebaruan dalam penelitian ini karena meneliti tentang hubungan aktifitas fisik dengan kualitas hidup kesehatan remaja di Kabupaten Minahasa Selatan. Tujuan penelitian ini ialah untuk mengalisa hubungan aktifitas fisik dengan kualitas hidup kesehatan remaja mulai dari kesehatan fisik, psikologis, sosial dan kesehata lingkungan di Kabupaten Minahasa Selatan. Penelitian ini menggunakan metode penelitian kuantitatif dengan pendekatan cross-sectional yang dilakukan pada bulan September sampai Desember 2021 di SMP dan SMA dengan jumlah sampel 431 responden menggunaka uji Kruskall Wallis. Hasil penelitian menunjukan bahwa untuk domain fisik p=0,863; domain psikologis p=0,967; domain sosial p=0,638; dan domain lingkungan p=0,725. Kesimpulan penelitian ini yaitu aktifitas fisik tidak berhubungan dengan kualitas hidup kesehatan remaja di Kabupaten Minahasa SelatanKata Kunci : Aktifitas fisik; Kualitas Hidup, Remaja AbstractCurrently, quality of life is one of the criteria used to evaluate health care interventions. Adolescents are one of the determinants in improving the quality of life of the community. The novelty of this study is that it examines the relationship between physical activity and the quality of life of adolescent health in the South Minahasa Regency. The purpose of this study is to analyze the relationship between physical activity and the quality of life of adolescent health starting from physical, psychological, social, and environmental health in the South Minahasa Regency. This research uses quantitative research methods with a cross-sectional approach conducted from September to December 2021 in junior and senior high schools with a total sample of 431 respondents using the Kruskal Wallis test. The results showed that for the physical domain p = 0.863; psychological domain p=0.967; social domain p=0.638; and the environment domain p=0.725. The conclusion of this study is that physical activity is not related to the quality of life of adolescent health in South Minahasa RegencyKeywords: Physical activity; Quality of Life, Teen

Molecular Insight into the Pharmacological Potential of <i>Clerodendrum minahassae</i> Leaf Extract for Type-2 Diabetes Management Using the Network Pharmacology Approach

Background and Objectives: The increasing occurrence and prevalence of type-2 diabetes mellitus (T2DM) have led to a growing interest in researching available treatment alternatives. Clerodendrum minahassae, a native plant species of North Sulawesi, has been a focus of ethnopharmacological studies due to its significance contributions to drug development, particularly its potential antidiabetic properties. This study investigated the pharmacological potential of Clerodendrum minahassae (CM) leaf extract for managing type-2 diabetes (T2DM) using a network pharmacology approach. Materials and Methods: Active compounds were extracted from CM leaves, and their interactions with target proteins in T2DM were explored through various in silico analyses. Results: SAR analysis using Way2Drug Pass Online identified 29 bioactive CM leaf extract compounds with promise as T2DM treatments. Additionally, 26 of these met Ro5 criteria for favorable drug-likeness. Most compounds exhibited positive pharmacodynamic and pharmacokinetic profiles, with 22 considered safe, while 7 posed potential toxicity risks when ingested individually. CM leaf extract targeted 60 T2DM-related proteins, potentially affecting T2DM via cytokine regulation, particularly in proteins linked to metabolic processes, cellular response to angiotensin, and the sphingosine-1-phosphate signaling pathway. The network pharmacology analysis identified five genes targeted by CM leaf extract, namely, STAT3, MAPK1, ESR1, PIK3R1, and NFKB1. Among these genes, PIK3R1’s interaction with the insulin receptor (INSR) positions it as a crucial candidate gene due to its pivotal role in insulin signal transduction during T2DM development. Conclusions: This research sheds light on the therapeutic potential of CM leaf extract for treating T2DM. This potential is attributed to the diverse array of bioactive compounds present in the extract, which have the capacity to interact with and inhibit proteins participating in the insulin signal transduction pathway crucial for the progression of T2DM. The findings of this study may open up possibilities for future applications of CM leaf extract in the development of novel T2DM treatments

In Vitro Test of Antioxidant Activity of Leilem Leaf Ethanol Extract (Clerodendrum minahassae) Using DPPH and FRAP Methods

The risk of degenerative diseases is increasing due to unhealthy lifestyles. Many people consume junk food, smoke, and do not protect themselves from sun exposure and pollution, exposing their bodies to free radicals. Free radicals can cause oxidative stress, necessitating the need for antioxidants to neutralize them. The leilem plant (Clerodendrum minahassae) is an endemic plant from North Sulawesi that is often used in traditional dishes and medicine. Moreover, leilem leaves are believed to be a source of natural antioxidants. This study aimed to determine the efficacy of leilem leaf ethanol extract as a source of antioxidants. The antioxidant potential was evaluated using phytochemical screening and antioxidant assays such as 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods, with ascorbic acid as a comparison. The level of antioxidant activity was measured by determining the IC50 value, which corresponds to the concentration of extract required to inhibit 50% of free radicals. The results of phytochemical screening showed that the ethanol extract of leilem (Clerodendrum minahassae) leaves contains active compounds such as phenols, flavonoids, saponins, tannins, steroids/triterpenoids, and alkaloids, indicating a high potential for antioxidant activity. The IC50 value for the DPPH method was 78.799 ppm, and the IC50 value for the FRAP method was 92.127 ppm. These results demonstrate that the ethanol extract of leilem leaves has strong antioxidant activity in both the DPPH and FRAP methods