5 research outputs found
Development of the bioartificial vaginal wall: An in vitro stage
Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9Β±32.5 ΞΌm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition. Β© Bionika Media Ltd
Π ΠΠΠ ΠΠΠΠ’ΠΠ ΠΠΠΠΠ Π’ΠΠ€ΠΠ¦ΠΠΠΠ¬ΠΠΠ Π‘Π’ΠΠΠΠ ΠΠΠΠΠΠΠΠ©Π: IN VITRO Π‘Π’ΠΠΠΠ―
Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9Β±32.5 ΞΌm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition. Β© Bionika Media Ltd.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π½Π΅ΡΠΊΠ°Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½ΠΎΠ²ΠΎΠ³ΠΎ Π½ΠΎΡΠΈΡΠ΅Π»Ρ, ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²Π»Π°Π³Π°Π»ΠΈΡΠ°. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠ»ΠΎΠΊΠ½ΠΈΡΡΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π° ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ. ΠΠ»Ρ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π²ΡΠ΅ΠΉ ΡΠΎΠ»ΡΠΈ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄, Π΄Π»Ρ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π²Π»Π°Π³Π°Π»ΠΈΡΠ° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ - ΡΡΠ°ΡΠΈΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π²Π»Π°Π³Π°Π»ΠΈΡΠ° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ EpCAM ΠΈ p63, ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π²ΠΈΠΌΠ΅Π½ΡΠΈΠ½ ΠΈ Π³Π»Π°Π΄ΠΊΠΎΠΌΡΡΠ΅ΡΠ½ΡΠΉ Π°ΠΊΡΠΈΠ½. ΠΠΎΡΠ»Π΅ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π±ΡΠ»ΠΈ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ Π² ΠΌΠ°ΡΡΠΈΠΊΡΠ΅ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ 1,5 ΠΌΠΌ; ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠ°ΡΠΏΠΎΠ»Π°Π³Π°Π»ΠΈΡΡ ΠΏΠ»ΠΎΡΠ½ΡΠΌ ΠΏΠ»Π°ΡΡΠΎΠΌ Π½Π° Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ, ΠΏΠΎΠ³ΡΡΠΆΠ°ΡΡΡ Π½Π° Π³Π»ΡΠ±ΠΈΠ½Ρ 88,9Β±32,5 ΠΌΠΊΠΌ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½Π°Ρ ΡΠΊΠ°Π½Π΅ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½Π°Ρ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ Π±Π»ΠΈΠ·ΠΊΠ° ΠΏΠΎ ΡΠ²ΠΎΠ΅ΠΉ Π°ΡΡ
ΠΈΡΠ΅ΠΊΡΠΎΠ½ΠΈΠΊΠ΅ ΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΌΡ ΡΠΎΡΡΠ°Π²Ρ Π½Π°ΡΠΈΠ²Π½ΠΎΠΌΡ Π²Π»Π°Π³Π°Π»ΠΈΡΡ
Nonwoven polycaprolactone scaffolds for tissue engineering: The choice of the structure and the method of cell seeding
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions
ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π° Π΄Π»Ρ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΈΠΈ: Π²ΡΠ±ΠΎΡ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΡΠΏΠΎΡΠΎΠ±Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ
Nonwoven polycaprolactone materials produced by electrospinning are perspective internal prosthetic implants. Seeding these implants with multipotent mesenchymal stromal cells stimulates the replacement of the prosthesis with recipient's own connective tissue. Electrospinning method was used for producing polycaprolactone matrices differing in thickness, pore diameter, fiber size, and biomechanical properties. Labeled cells were seeded on scaffolds in three ways: (1) static, (2) dynamic, and (3) directed flow of the cell suspension generated by capillary action. Cell distribution on the surface and the interior of the scaffolds was studied; the metabolic activity of cells was measured by MTT assay. Static seeding method yielded fully confluence of cells covered the entire scaffold surface, but the cells were located primarily in the upper third of the matrix. Dynamic method proved to be effective only for scaffolds of thickness greater than 500 microns, irrespective of the pore diameter. The third method was effective only for scaffolds with the pore diameter of 20-30 microns, regardless of the material thickness. Resorbable nonwoven polycaprolactone electrospun materials have appropriate biomechanical properties and similar to native tissue matrix structures for internal prosthesis. The choice of the most effective cell seeding method depends on the spatial characteristics - the material thickness, pore diameter, and fibers size, which are determined by the electrospinning conditions.ΠΠ΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΡΠ²Π»ΡΡΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ°ΠΌΠΈ Π΄Π»Ρ ΡΠ½Π΄ΠΎΠΏΡΠΎΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΠΊΠΈΡ
ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π·Π°ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅Π·Π° ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΎΠ΅Π΄ΠΈΠ½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΡΡ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ°. Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ΅Ρ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π½Π΅ΡΠΊΠ°Π½ΡΡ
Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠ° ΠΏΠΎΠ»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½ΠΎΠ²ΡΡ
ΠΌΠ°ΡΡΠΈΡ, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΡ
ΡΡ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ. ΠΠ°ΡΠ΅Π»Π΅Π½ΠΈΠ΅ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΌΠ΅ΡΠ΅Π½ΡΠΌΠΈ ΠΌΡΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΠΌΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΏΡΠΏΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°ΡΠΈΠΊΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΡΠ΅ΠΌΡ ΡΠΏΠΎΡΠΎΠ±Π°ΠΌΠΈ: ΡΡΠ°ΡΠΈΡΠ½ΡΠΌ, Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ°. ΠΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΈ ΡΠΎΠ»ΡΠΈΠ½Π΅ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ², ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ·ΠΌΠ΅ΡΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΠ’Π’-ΡΠ΅ΡΡΠ°. Π‘ΡΠ°ΡΠΈΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» ΠΏΠΎΠ»ΡΡΠΈΡΡ Π½ΠΎΡΠΈΡΠ΅Π»ΠΈ Ρ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΡΠΌ ΠΏΠΎΠΊΡΡΡΠΈΠ΅ΠΌ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΊΠ»Π΅ΡΠΊΠΈ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ ΡΠ°ΡΠΏΠΎΠ»Π°Π³Π°Π»ΠΈΡΡ Π² Π²Π΅ΡΡ
Π½Π΅ΠΉ ΡΡΠ΅ΡΠΈ ΠΌΠ°ΡΡΠΈΠΊΡΠ°. ΠΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠΊΠ°Π·Π°Π»ΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ Π±ΠΎΠ»Π΅Π΅ 500 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ. ΠΠ΅ΡΠΎΠ΄ Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° Π±ΡΠ» ΡΡΡΠ΅ΠΊΡΠΈΠ²Π΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Π΄Π»Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Ρ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡ 20-30 ΠΌΠΊΠΌ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΠΈΠΎΡΠ΅Π·ΠΎΡΠ±ΠΈΡΡΠ΅ΠΌΡΠ΅ Π½Π΅ΡΠΊΠ°Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎ-Π»ΠΈΠΊΠ°ΠΏΡΠΎΠ»Π°ΠΊΡΠΎΠ½Π°, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠΈΠΌΠΈ Π±ΠΈΠΎΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ Π΄Π»Ρ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΡΡΠ΅Π½ΠΎΠΊ Π±ΡΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΡΠΈ, ΠΈΠΌΠ΅ΡΡ ΡΡ
ΠΎΠ΄Π½ΠΎΠ΅ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠΌ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅. ΠΡΠ±ΠΎΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° Π·Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ Π΅Π³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ - ΡΠΎΠ»ΡΠΈΠ½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°, Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΏΠΎΡ ΠΈ Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, ΠΊΠΎΡΠΎΡΡΠ΅, Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΡΡ ΡΡΠ»ΠΎΠ²ΠΈΡΠΌΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠΌΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°
Bioengineered Bile Duct: the project resume and state of the art in 2018
Aim. Development of the physiologically relevant tissue-engineered graft for repair of bile duct injures (Dyuzheva et al., 2016). <br><br>Conclusions. Designed scaffold showed no cytotoxicity, both BM-MSCs and cholangiocytes migrated into the depth of fibrous material. The constructs was biodegradable in various model mediums: deionized water, phosphate buffer, bile, full culture medium. The next step is pre-clinical trials on rabbits and minipigs for assessment of implantation safety and efficacy. We suppose that this tubular multilayered tissue-engineered construct will be capable to integration in native tissues after implantation and may be used to injured bile duct reparation