The one-dimensional (1D) ddPCR results from Bio-Rad QX100TM Droplet Reader of <i>18S rRNA Plasmodium</i> genus detection and quantification assay.

<p>The different amplitudes of positive droplets were observed when different PCR annealing temperatures were applied (A10: 65°C, D10: 62°C, E10: 60°C, F10: 59°C, H10: 57°C, F09: 60°C for negative control). Threshold for positive detection is 2500.</p

Workflow of the study.

<p>Workflow of the study.</p

Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).

<p>Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).</p

Probit analysis.

<p>The 95% probability of detecting parasitaemia as low as 10.674 parasites/mL when high volume of blood samples was applied with <i>Plasmodium</i> genus ddPCR assay.</p

The column bar graphs show <i>18S rRNA</i> copies/mL of blood obtained from single reaction ddPCR and duplex ddPCR assays.

<p>Mean 95% CI of <i>18S rRNA</i> of <i>P</i>. <i>falciparum</i> (5a), <i>P</i>. <i>vivax</i> (5b), <i>P</i>. <i>malariae</i> (5c) and <i>P</i>. <i>ovale</i> (5d).</p

MOESM2 of Plasmodium falciparum Kelch 13 mutations and treatment response in patients in Hpa-Pun District, Northern Kayin State, Myanmar

Additional file 2. Parasite clearance curves for individual patients

MOESM1 of Plasmodium falciparum Kelch 13 mutations and treatment response in patients in Hpa-Pun District, Northern Kayin State, Myanmar

Additional file 1. Genetic markers of artemisinin resistance

MOESM3 of Genetic polymorphisms in the circumsporozoite protein of Plasmodium malariae show a geographical bias

Additional file 3. NAPG tetrapeptide repeats in Plasmodium malariae field isolates from Thailand, Myanmar, Lao PDR, and Bangladesh

Patterns of allelic diversity in the Kolkata <i>P. vivax</i> population.

<p>1. Three antigenic and 8 microsatellite genetic loci.</p><p>2. He: heterozygosity.</p><p>3. The alleles were numbered and the number of the most commonly identified allele and the corresponding proportion (%) of all identified alleles are shown. When two are equal, both are shown.</p

MOESM4 of Genetic polymorphisms in the circumsporozoite protein of Plasmodium malariae show a geographical bias

Additional file 4. Average number of the tetrapeptide repeats: NAAG and NDAG, between the Asian and African samples (A) at the country level and (B) at the continent level