A new SYBR green real-time PCR assay for semi-quantitative detection of Echinococcus multilocularis and Echinococcus canadensis DNA on bilberries (Vaccinium myrtillus) : Food and Waterborne Parasitology

Berries and vegetables are potential transmission vehicles for eggs of pathogenic parasites, such as Echinococcus spp. We developed a SYBR Green based semi-quantitative real-time PCR (qPCR) method for detection of Echinococcus multilocularis and Echinococcus canadensis DNA from berry samples. A set of primers based on the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene was designed and evaluated. To assess the efficacy of the assay, we spiked bilberries (Vaccinium myrtillus) with a known amount of E. multilocularis eggs. The detection limit for the assay using the NAD1_88 primer set was 4.37 × 10−5 ng/μl of E. multilocularis DNA. Under artificial contamination of berries, 50 E. multilocularis eggs were reliably detected in 250 g of bilberries. Analytical sensitivity of the assay was determined to be 100% with three eggs. As an application of the assay, 21 bilberry samples from Finnish market places and 21 bilberry samples from Estonia were examined. Previously described sieving and DNA extraction methods were used, and the samples were analyzed for E. multilocularis and E. canadensis DNA using semi-quantitative real-time PCR and a melting curve analysis of the amplified products. Echinococcus DNA was not detected in any of the commercial berry samples. This easy and fast method can be used for an efficient detection of E. multilocularis and E. canadensis in bilberries or other berries, and it is applicable also for fruits and vegetables. © 2019 The AuthorsPeer reviewe

Non-tuberculous Mycobacteria can Cause Disseminated Mycobacteriosis in Cats

Mycobacteriosis caused by non-tuberculous mycobacteria (NTM) is a rising concern in human medicine both in immunocompromised and immunocompetent patients. In cats, mycobacteriosis caused by NTM is considered mostly to be a focal or dermal infection, with disseminated disease mostly caused by Mycobacterium avium. We describe three cases of disseminated mycobacteriosis in cats, caused by Mycobacterium malmoense, Mycobacterium branderi/shimoidei and M. avium, with no identified underlying immunosuppression. In all cases, extracellular mycobacteria were seen in the pulmonary epithelium, intestinal lumen and glomerular tufts, which could affect the shedding of the organism. The present study highlights the importance of mycobacteriosis as a differential even in immunocompetent animals. Considering the close relationship of owners and pets and the potential presence of free mycobacteria in secretions, cats should be considered as a possible environmental reservoir for mycobacteria. (C) 2018 Elsevier Ltd. All rights reserved.Peer reviewe

Epidemic situation of cestodiasis in domestic reindeer on reindeer farms in the Murmansk Oblast

The paper presents the results of the cestodiasis epidemic situation in domestic reindeer in the farms of the Murmansk Oblast. The studies were performed in 2018–2019 during the routine slaughter of reindeer at slaughter houses APC “Tundra” and APC HFE SEN “Olenevod” located in settlements Lovozero, Krasnoschelye, and Sosnovka. Totally 4,048 carcasses of domestic reindeer were tested, 2,812 out of them – in Lovozero, 396 – in Sosnovka, and 840 – in Krasnoschelye. During the meat inspection the parenchymal organs were examined for cestode cysts. When detected they were sampled and gross specimens were prepared using standard parasitological methods. 56 samples of internal organs of deer suspected in tapeworm infestation were collected from the inspected carcasses, in 25 of them tapeworms were detected and in the rest of the samples parasites were not detected. The tapeworm species were determined at the Department of Veterinary Biosiences, Faculty of Veterinary Medicine, University of Helsinki. The test performed revealed echinococcosis (Echinococcus canadensis) and cysticercosis (Taenia hydatigena). Most lesions were detected in liver where the agent’s larvae cysts are observed. It was established that the level of domestic reindeer infestation with the agents of cysticercosis in APC “Tundra” was 0.5%, echinococcosis – 0.04%, in APC HFE SEN “Olenevod” cysticercosis was diagnosed in 0.81% cases, echinococcosis was not detected. On the whole 0.62% of reindeer on reindeer farms were infested with cestodes. Measures taken for prevention of helminth infestation in domestic and farm animals bear good results

A new SYBR green real-time PCR assay for semi-quantitative detection of Echinococcus multilocularis and Echinococcus canadensis DNA on bilberries (Vaccinium myrtillus) : Food and Waterborne Parasitology

Berries and vegetables are potential transmission vehicles for eggs of pathogenic parasites, such as Echinococcus spp. We developed a SYBR Green based semi-quantitative real-time PCR (qPCR) method for detection of Echinococcus multilocularis and Echinococcus canadensis DNA from berry samples. A set of primers based on the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene was designed and evaluated. To assess the efficacy of the assay, we spiked bilberries (Vaccinium myrtillus) with a known amount of E. multilocularis eggs. The detection limit for the assay using the NAD1_88 primer set was 4.37 × 10−5 ng/μl of E. multilocularis DNA. Under artificial contamination of berries, 50 E. multilocularis eggs were reliably detected in 250 g of bilberries. Analytical sensitivity of the assay was determined to be 100% with three eggs. As an application of the assay, 21 bilberry samples from Finnish market places and 21 bilberry samples from Estonia were examined. Previously described sieving and DNA extraction methods were used, and the samples were analyzed for E. multilocularis and E. canadensis DNA using semi-quantitative real-time PCR and a melting curve analysis of the amplified products. Echinococcus DNA was not detected in any of the commercial berry samples. This easy and fast method can be used for an efficient detection of E. multilocularis and E. canadensis in bilberries or other berries, and it is applicable also for fruits and vegetables. © 2019 The AuthorsPeer reviewe

Enabling the Galileo high accuracy service with open-source software: integration of HASlib and RTKLIB

The Galileo high accuracy service (HAS) is a free-of-charge service designed to deliver decimeter-level accuracy in real-time precise point positioning (PPP) applications using global navigation satellite systems (GNSS). With the intention of facilitating the use of HAS corrections with open access tools, we present the open-source library named HASlib and its integration with another open-source library named RTKLIB. HASlib decodes the Reed–Solomon encoded Galileo E6 navigation data pages and outputs the corrections in commonly used formats. This enables the utilization of HAS with conventional GNSS receivers and PPP engines that lack native support for HAS formats. For instance, the outputs from HASlib enable the use of HAS corrections in RTKLIB. In order to validate this integration, we demonstrate that HAS can allow decimeter-level accuracy using only free-of-charge services and tools. We have obtained a 3D root mean square error below 20 cm (1 sigma) after a convergence time of 10–90 min in Finland. This accuracy has overcome classical real-time solutions with broadcast and satellite-based augmentation system (SBAS) data by one order of magnitude. Compared to post-processed multi-GNSS PPP, HAS corrections required longer convergence times, given the real-time nature. Furthermore, our assessment revealed that the longer convergence time, compared to prior literature, was attributed to RTKLIB filtering procedure and geometry deficiencies in high latitudes. Nevertheless, once convergence was attained, a sub-decimeter level of accuracy was observed in both horizontal and vertical components. These findings highlight the effectiveness of Galileo HAS, HASlib, and RTKLIB as powerful tools for providing open-access to real-time PPP solutions

The Bologna Stone: history's first persistent luminescent material

In 1603, the Italian shoemaker Vincenzo Cascariolo found that a stone (baryte) from the outskirts of Bologna emitted light in the dark without any external excitation source. However, the calcination of the baryte was needed prior to this observation. The stone later named as the Bologna Stone was among the first luminescent materials and the first documented material to show persistent luminescence. The mechanism behind the persistent emission in this material has remained a mystery ever since. In this work, the Bologna Stone (BaS) was prepared from the natural baryte (Bologna, Italy) used by Cascariolo. Its properties, e. g. impurities (dopants) and their valences, luminescence, persistent luminescence and trap structure, were compared to those of the pure BaS materials doped with different (transition) metals (Cu, Ag, Pb) known to yield strong luminescence. The work was carried out by using different methods (XANES, TL, VUV-UV-vis luminescence, TGA-DTA, XPD). A plausible mechanism for the persistent luminescence from the Bologna Stone with Cu+ as the emitting species was constructed based on the results obtained. The puzzle of the Bologna Stone can thus be considered as resolved after some 400 years of studies