Xanthurenic acid translocates proapoptotic Bcl-2 family proteins into mitochondria and impairs mitochondrial function

BACKGROUND: Xanthurenic acid is an endogenous molecule produced by tryptophan degradation, produced in the cytoplasm and mitochondria. Its accumulation can be observed in aging-related diseases, e.g. senile cataract and infectious disease. We previously reported that xanthurenic acid provokes apoptosis, and now present a study of the response of mitochondria to xanthurenic acid. RESULTS: Xanthurenic acid at 10 or 20 μM in culture media of human aortic smooth muscle cells induces translocation of the proteins Bax, Bak, Bclx(s), and Bad into mitochondria. In 20 μM xanthurenic acid, Bax is also translocated to the nucleus. In isolated mitochondria xanthurenic acid leads to Bax and Bclx(s )oligomerization, accumulation of Ca(2+), and increased oxygen consumption. CONCLUSION: Xanthurenic acid interacts directly with Bcl-2 family proteins, inducing mitochondrial pathways of apoptosis and impairing mitochondrial functions

Variation in sport participation, fitness and motor coordination with socioeconomic status among Flemish children

Socioeconomic status (SES) is often indicated as a factor that influences physical activity and associated health outcomes. This study examined the relationship between SES and sport participation, morphology, fitness and motor coordination in a sample of 1955 Flemish children 6-11 years of age. Gender, age and SES-specific values for morphologic dimensions, amount and type of sport participation and fitness and motor coordination tests were compared. SES was positively and significantly associated with sport participation and sports club membership in both sexes. Although differences were not consistently significant, morphologic dimensions and tests of fitness and motor coordination showed a trend in favor of children from higher SES. The results suggest that public and local authorities should consider providing equal opportunities for children in all social strata and especially those in the lower SES to experience the beneficial effects of sport participation through which they can enhance levels of physical fitness and motor coordination

Assessment of reliability in isokinetic testing among adolescent basketball players

Background. The reproducibility of day-to-day testing of isokinetic concentric and eccentric muscular actions among adolescent basketball players aged 14 to 16 years and relationships of mean within-subject variation in two isokinetic testing sessions with chronological age, biological maturation (estimated age at peak height velocity), training experience, body size, lower-body morphology, and initial strength performance were evaluated. Material and Methods. The sample included 27 basketball players who completed replicate test sessions of 5 repetitions of reciprocal concentric and eccentric knee extensions and flexions at 60 degrees s(-1). A randomly selected subsample of 8 players completed a third testing session to confirm reliability estimates. Results. Coefficients of variation (CV) between sessions 1 and 2 ranged from 8.1% to 17.4%, and intraclass coefficients (ICCs) ranged from 0.72 to 0.89. For sessions 1 and 3, CVs ranged from 3.9% to 6.0%, and ICCs ranged from 0.95 to 0.99. The initial level of strength of eccentric knee flexion (r=-0.43) and eccentric knee extension (r=-0.42) were correlated (P<0.05) with eccentric knee extension within-variation between two sessions. Training experience (r=-0.37, P<0.05) and initial values of concentric knee flexion (r=-0.62, P<0.01) were correlated with concentric knee flexion within-subject differences. Within-subject variation of eccentric knee extension was correlated (P<0.05) with chronologic age (r=0.41), estimated age at peak height velocity (r=-0.38), body size (r=0.41 to 0.47), and leg volume (r=0.39). Conclusions. Familiarization sessions may improve the reliability of concentric and eccentric knee isokinetic strength testing at 60 s(-1) in adolescent basketball players. Age, maturity status, and training experience of young athletes should be considered when testing knee isokinetic strength at 60 degrees s(-1)

A prospective analysis of the injury incidence of young male professional football players on artificial turf

Background: The effects of synthetic surfaces on the risk of injuries is still debated in literature and the majority of published data seems to be contradictory. For such reasons the understanding of injury incidence on such surfaces, especially in youth sport, is fundamental for injury prevention. Objectives: The aim of this study was to prospectively report the epidemiology of injuries in young football players, playing on artificial turfs, during a one sports season. Patients and Methods: 80 young male football players (age 16.1 ± 3.7 years; height 174 ± 6.6 cm; weight 64.2 ± 6.3 kg) were enrolled in a prospective cohort study. The participants were then divided in two groups; the first included players age ranging from 17 to 19 (OP) whereas the second included players age ranging from 13 to 16 (YP). Injury incidence was recorded prospectively, according to the consensus statement for soccer. Results: A total of 107 injuries (35 from the OP and 72 from the YP) were recorded during an exposure time of 83.760 hours (incidence 1.28/1000 per player hours); 22 during matches (incidence 2.84/1000 per player hours, 20.5%) and 85 during training (incidence 1.15/1000 per player hours, 79.5%). Thigh and groin were the most common injury locations (33.6% and 21.5%, respectively) while muscle injuries such as contractures and strains were the most common injury typologies (68.23%). No statistical differences between groups were displayed, except for the rate of severe injuries during matches, with the OP displaying slightly higher rates compared to the YP. Severe injuries accounted for 10.28% of the total injuries reported. The average time lost due to injuries was 14 days. Re-injuries accounted for 4.67% of all injuries sustained during the season. Conclusions: In professional youth soccer injury rates are reasonably low. Muscle injuries are the most common type of injuries while groin and thigh the most common locations. Artificial turf pitches don’t seem to contribute to injury incidence in young football players

Tranzicija u Željezari Sisak

Transition coupled with agression towards Croatia, as well as the collapse of the former market have brought a series of dramatical and unplanned changes to Željezara Sisak. The much, needed business transformation is being achieved unsuccessfully by developing a series of Programs, hiring international management consultants as well as strategic business partners. However, due to the failure of previously set strategy the possible company recovery also fails, and Željezara Sisak ends up bancrupt. Because the first attempt at privatization was not successful, Željezara Sisak is now faced with a difficult decision regarding the choice of its new strategic partner/owner.Agresija na Hrvatsku i raspad bivšeg tržišta donosi niz dramatičnih, neplaniranih promjena u Željezari Sisak. Preokret u poslovanju pokušava se ostvariti izradom niza Programa restrukturiranja, angažiranjem inozemnih konzultanata i strateških partnera. Nažalost, zbog odustajanja od utvrđene strategije propada sanacija i Željezara Sisak završava u stečaju. Privatizacijom se u prvom pokušaju ne uspijeva, te je pred Željezarom Sisak presudna odluka o izboru novog strateškog partnera/vlasnika

Spatially-explicit land use change emissions and carbon payback times of biofuels under the Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA)

The Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA) requires airlines to offset their greenhouse gas (GHG) emissions above 2019 levels by either buying carbon offsets or using Sustainable Aviation Fuels (SAFs). These are drop-in jet fuels made from biomass or other renewable resources that reduce GHG emissions by at least 10 % compared to kerosene and meet certain sustainability criteria. This study assesses the direct land use change (DLUC) emissions of SAF, i.e., GHG emissions from on-site land conversion from previous uses (excluding primary forests, peatlands, wetlands, and protected and biodiversity-rich areas) into alternative feedstocks, considering spatial variability in global yields and land carbon stocks. The results provide DLUC values and carbon payback times at 0.5-degree resolution for six SAF pathways, with and without irrigation and a medium-input intensity, according to CORSIA sustainability criteria. When excluding CORSIA non-compliant areas, soybean SAF shows the highest mean DLUC factor (31.9 ± 20.7 gCO2/MJ), followed by reed canary grass and maize. Jatropha SAF shows the lowest mean DLUC factor (3.6 ± 31.4 gCO2/MJ), followed by miscanthus and switchgrass. The latter feedstocks show potential for reducing GHG emissions over large areas but with relatively greater variability. Country-average DLUC values are higher than accepted ILUC ones for all pathways except for maize. To ensure the GHG benefits of CORSIA, feedstocks must be produced in areas where not only carbon stocks are relatively low but also where attainable yields are sufficiently high. The results help identify locations where the combination of these two factors may be favourable for low-DLUC SAF production. Irrigated miscanthus offers the highest SAF production potential (2.75 EJ globally) if grown on CORSIA-compliant cropland and grassland areas, accounting for ∼1/5 of the total kerosene used in 2019. Quantifying other environmental impacts of SAFs is desirable to understand sustainability trade-offs and financial constraints that may further limit production potentials. © 2024 The Author(s)All co-authors acknowledge the financial support of the EU H2020 project ALTERNATE “Assessment on Alternative Aviation Fuels Development” (GA # 875538) and the MSCA-IF-2020 project GIFTS “Global Interlinkages in Food Trade Systems” (GA #101029457). This research is also supported by María de Maeztu Excellence Unit 2023- 2027 Ref. CEX2021-001201-M, funded by MCIN/AEI/10.130 39/501100011033; and by the Basque Government through the BERC 2022-2025 program. All co-authors thank Dr. Hugo Valin for his initial contributions to the analysis for this publication

Lens epithelial cell apoptosis and intracellular Ca(2+) increase in the presence of xanthurenic acid

BACKGROUND: Xanthurenic acid is an endogenous product of tryptophan degradation by indoleamine 2,3-dioxygenase (IDO). We have previously reported that IDO is present in mammalian lenses, and xanthurenic acid is accumulated in the lenses with aging. Here, we studied the involvement of xanthurenic acid in the human lens epithelial cell physiology. METHODS: Human lens epithelial cells primary cultures were used. Control cells, and cells in the presence of xanthurenic acid grow in the dark. Western blot analysis and immunofluorescence studies were performed. RESULTS: In the presence of xanthurenic acid human lens epithelial cells undergo apoptosis-like cell death. In the control cells gelsolin stained the perinuclear region, whereas in the presence of 10 μM xanthurenic acid gelsolin is translocated to the cytoskeleton, but does not lead to cytoskeleton breakdown. In the same condition caspase-3 activation, and DNA fragmentation was observed. At low (5 to 10 μM) of xanthurenic acid concentration, the elongation of the cytoskeleton was associated with migration of mitochondria and cytochrome c release. At higher concentrations xanthurenic acid (20 μM and 40 μM) damaged mitochondria were observed in the perinuclear region, and nuclear DNA cleavage was observed. We observed an induction of calpain Lp 82 and an increase of free Ca(2+) in the cells in a xanthurenic acid concentration-dependent manner. CONCLUSIONS: The results show that xanthurenic acid accumulation in human lens epithelial cells disturbs the normal cell physiology and leads to a cascade of pathological events. Xanthurenic acid induces calpain Lp82 and caspases in the cells growing in the dark and can be involved in senile cataract development