3 research outputs found
Specific Proteomic Response of <i>Unio pictorum</i> Mussel to a Mixture of Glyphosate and Microcystin-LR
Cyanobacterial
toxins and pesticides regularly impact freshwaters.
Microcystin-LR is one of the most toxic and common cyanobacterial
toxins whereas glyphosate is the active ingredient of a widely use
herbicide. As filter feeders, freshwater mussels are particularly
exposed. Like many native bivalve species, <i>Unio pictorum</i> suffers from a continuous decline in Europe. In order to get a deeper
insight of its response to contaminants, <i>U. pictorum</i> was exposed to either 10 Ī¼g L<sup>ā1</sup> of microcystin-LR
or 10 Ī¼g L<sup>ā1</sup> of glyphosate or a mixture of
both. Proteins of the digestive glands were extracted and analyzed
by DIGE. Gel analysis revealed 103 spots with statistical variations,
and the response seems to be less toward glyphosate than to microcystin-LR.
Specific spots have variations only when exposed to the mixture, showing
that there is an interaction of both contaminants in the responses
triggered. The proteins of 30 spots have been identified. They belong
mostly to the cytoskeleton family, but proteins of the oxidative pathway,
detoxification, and energetic metabolism were affected either by glyphosate
or microcystin-LR or by the mixture. These results demonstrate the
importance to study contaminants at low concentrations representative
of those found in the field and that multicontaminations can lead
to different response pathways
Specific Proteomic Response of <i>Unio pictorum</i> Mussel to a Mixture of Glyphosate and Microcystin-LR
Cyanobacterial
toxins and pesticides regularly impact freshwaters.
Microcystin-LR is one of the most toxic and common cyanobacterial
toxins whereas glyphosate is the active ingredient of a widely use
herbicide. As filter feeders, freshwater mussels are particularly
exposed. Like many native bivalve species, <i>Unio pictorum</i> suffers from a continuous decline in Europe. In order to get a deeper
insight of its response to contaminants, <i>U. pictorum</i> was exposed to either 10 Ī¼g L<sup>ā1</sup> of microcystin-LR
or 10 Ī¼g L<sup>ā1</sup> of glyphosate or a mixture of
both. Proteins of the digestive glands were extracted and analyzed
by DIGE. Gel analysis revealed 103 spots with statistical variations,
and the response seems to be less toward glyphosate than to microcystin-LR.
Specific spots have variations only when exposed to the mixture, showing
that there is an interaction of both contaminants in the responses
triggered. The proteins of 30 spots have been identified. They belong
mostly to the cytoskeleton family, but proteins of the oxidative pathway,
detoxification, and energetic metabolism were affected either by glyphosate
or microcystin-LR or by the mixture. These results demonstrate the
importance to study contaminants at low concentrations representative
of those found in the field and that multicontaminations can lead
to different response pathways