73 research outputs found
Preparation of Isotopically Labeled Ribonucleotides for Multidimensional NMR Spectroscopy of RNA
A general method for large scale preparation of uniformly isotopically labeled ribonucleotides and RNAs is described. Bacteria are grown on isotopic growth medium, and their nucleic acids are harvested and degraded to mononucleotides. These are enzymatically converted into ribonucleoside triphosphates, which are used in transcription reactions in vitro to prepare RNAs for NMR studies. For 15N-labeling, E.coli is grown on15N-ammonium sulfate, whereas for 13C-labeling, Methylophilus methylotrophus is grown on 13C-methanol, which is more economical than 13C-glucose. To demonstrate the feasibility and utility of this method, uniformly 13C-labeled ribonucleotides were used to synthesize a 31 nucleotide HIV TAR RNA that was analyzed by 3D-NMR. This method should find widespread use in the structural analysis of RNA by NMR
Gap Anisotropy and de Haas-van Alphen Effect in Type-II Superconductors
We present a theoretical study on the de Haas-van Alphen (dHvA) oscillation
in the vortex state of type-II superconductors, with a special focus on the
connection between the gap anisotropy and the oscillation damping. Numerical
calculations for three different gap structures clearly indicate that the
average gap along extremal orbits is relevant for the magnitude of the extra
damping, thereby providing a support for experimental efforts to probe gap
anisotropy through the dHvA signal. We also derive an analytic formula for the
extra damping which gives a good fit to the numerical results.Comment: 5 pages, 1 figure, changes in Introductio
SDSS J115517.35+634622.0: A Newly Discovered Gravitationally Lensed Quasar
We report the discovery of SDSSJ115517.35+634622.0, a previously unknown
gravitationally lensed quasar. The lens system exhibits two images of a quasar, with an image separation of 1{\farcs}832 \pm 0.007 . Near-IR
imaging of the system reveals the presence of the lensing galaxy between the
two quasar images. Based on absorption features seen in the Sloan Digital Sky
Survey (SDSS) spectrum, we determine a lens galaxy redshift of .
The lens is rather unusual in that one of the quasar images is only
0{\farcs}22\pm0{\farcs}07 () from the center of the
lens galaxy and photometric modeling indicates that this image is significantly
brighter than predicted by a SIS model. This system was discovered in the
course of an ongoing search for strongly lensed quasars in the dataset from the
SDSS.Comment: 18 pages, 6 figures. Accepted for publication in A
SDSS J092455.87+021924.9: an Interesting Gravitationally Lensed Quasar from the Sloan Digital Sky Survey
We report the discovery of a new gravitationally lensed quasar from the Sloan
Digital Sky Survey, SDSS J092455.87+021924.9 (SDSS J0924+0219). This object was
selected from among known SDSS quasars by an algorithm that was designed to
select another known SDSS lensed quasar (SDSS 1226-0006A,B). Five separate
components, three of which are unresolved, are identified in photometric
follow-up observations obtained with the Magellan Consortium's 6.5m Walter
Baade telescope at Las Campanas Observatory. Two of the unresolved components
(designated A and B) are confirmed to be quasars with z=1.524; the velocity
difference is less than 100 km sec^{-1} according to spectra taken with the W.
M. Keck Observatory's Keck II telescope on Mauna Kea. A third stellar
component, designated C, has the colors of a quasar with redshift similar to
components A and B. The maximum separation of the point sources is 1.78". The
other two sources, designated G and D, are resolved. Component G appears to be
the best candidate for the lensing galaxy. Although component D is near the
expected position of the fourth lensed component in a four image lens system,
its properties are not consistent with being the image of a quasar at z~1.5.
Nevertheless, the identical redshifts of components A and B and the presence of
component C strongly suggest that this object is a gravitational lens. Our
observations support the idea that a foreground object reddens the fourth
lensed component and that another unmodeled effect (such as micro- or
milli-lensing) demagnificates it, but we cannot rule out the possibility that
SDSS0924+0219 is an example of the relatively rare class of ``three component''
lens systems.Comment: 24 pages, 6 figures, accepted by A
Recommended from our members
The Arabidopsis thaliana nucleotide sugar transporter GONST2 is a functional homolog of GONST1.
Glycosylinositolphosphorylceramides (GIPCs) are the predominant lipid in the outer leaflet of the plasma membrane. Characterized GIPC glycosylation mutants have severe or lethal plant phenotypes. However, the function of the glycosylation is unclear. Previously, we characterized Arabidopsis thaliana GONST1 and showed that it was a nucleotide sugar transporter which provides GDP-mannose for GIPC glycosylation. gonst1 has a severe growth phenotype, as well as a constitutive defense response. Here, we characterize a mutant in GONST1's closest homolog, GONST2. The gonst2-1 allele has a minor change to GIPC headgroup glycosylation. Like other reported GIPC glycosylation mutants, gonst1-1gonst2-1 has reduced cellulose, a cell wall polymer that is synthesized at the plasma membrane. The gonst2-1 allele has increased resistance to a biotrophic pathogen Golovinomyces orontii but not the necrotrophic pathogen Botrytis cinerea. Expression of GONST2 under the GONST1 promoter can rescue the gonst1 phenotype, indicating that GONST2 has a similar function to GONST1 in providing GDP-D-Man for GIPC mannosylation
Postoperative Increase in Occiput–C2 Angle Negatively Impacts Subaxial Lordosis after Occipito–Upper Cervical Posterior Fusion Surgery
Study DesignRetrospective case series.PurposeTo elucidate the impact of postoperative occiput–C2 (O–C2) angle change on subaxial cervical alignment.Overview of LiteratureIn the case of occipito–upper cervical fixation surgery, it is recommended that the O–C2 angle should be set larger than the preoperative value postoperatively.MethodsThe present study included 17 patients who underwent occipito–upper cervical spine (above C4) posterior fixation surgery for atlantoaxial subluxation of various etiologies. Plain lateral cervical radiographs in a neutral position at standing were obtained and the O–C2 angle and subaxial lordosis angle (the angle between the endplates of the lowest instrumented vertebra (LIV) and C7 vertebrae) were measured preoperatively and postoperatively soon after surgery and ambulation and at the final follow-up visit.ResultsThere was a significant negative correlation between the average postoperative alteration of O–C2 angle (DO–C2) and the average postoperative alteration of subaxial lordosis angle (Dsubaxial lordosis angle) (r=–0.47, p=0.03).ConclusionsThere was a negative correlation between DO–C2 and Dsubaxial lordosis angles. This suggests that decrease of mid-to lower-cervical lordosis acts as a compensatory mechanism for lordotic correction between the occiput and C2. In occipito-cervical fusion surgery, care must be taken to avoid excessive O–C2 angle correction because it might induce mid-to-lower cervical compensatory decrease of lordosis
Theory of de Haas-van Alphen Effect in Type-II Superconductors
Theory of quasiparticle spectra and the de Haas-van Alphen (dHvA) oscillation
in type-II superconductors are developed based on the Bogoliubov-de Gennes
equations for vortex-lattice states. As the pair potential grows through the
superconducting transition, each degenerate Landau level in the normal state
splits into quasiparticle bands in the magnetic Brillouin zone. This brings
Landau-level broadening, which in turn leads to the extra dHvA oscillation
damping in the vortex state. We perform extensive numerical calculations for
three-dimensional systems with various gap structures. It is thereby shown that
(i) this Landau-level broadening is directly connected with the average gap at
H=0 along each Fermi-surface orbit perpendicular to the field H; (ii) the extra
dHvA oscillation attenuation is caused by the broadening around each extremal
orbit. These results imply that the dHvA experiment can be a unique probe to
detect band- and/or angle-dependent gap amplitudes. We derive an analytic
expression for the extra damping based on the second-order perturbation with
respect to the pair potential for the Luttinger-Ward thermodynamic potential.
This formula reproduces all our numerical results excellently, and is used to
estimate band-specific gap amplitudes from available data on NbSe_2, Nb_3Sn,
and YNi_2B_2C. The obtained value for YNi_2B_2C is fairly different from the
one through a specific-heat measurement, indicating presence of gap anisotropy
in this material. C programs to solve the two-dimensional Bogoliubov-de Gennes
equations are available at http://phys.sci.hokudai.ac.jp/~kita/index-e.html .Comment: 16 pages, 11 figure
Postoperative Increase in Occiput–C2 Angle Negatively Impacts Subaxial Lordosis after Occipito–Upper Cervical Posterior Fusion Surgery
Study DesignRetrospective case series.PurposeTo elucidate the impact of postoperative occiput–C2 (O–C2) angle change on subaxial cervical alignment.Overview of LiteratureIn the case of occipito–upper cervical fixation surgery, it is recommended that the O–C2 angle should be set larger than the preoperative value postoperatively.MethodsThe present study included 17 patients who underwent occipito–upper cervical spine (above C4) posterior fixation surgery for atlantoaxial subluxation of various etiologies. Plain lateral cervical radiographs in a neutral position at standing were obtained and the O–C2 angle and subaxial lordosis angle (the angle between the endplates of the lowest instrumented vertebra (LIV) and C7 vertebrae) were measured preoperatively and postoperatively soon after surgery and ambulation and at the final follow-up visit.ResultsThere was a significant negative correlation between the average postoperative alteration of O–C2 angle (DO–C2) and the average postoperative alteration of subaxial lordosis angle (Dsubaxial lordosis angle) (r=–0.47, p=0.03).ConclusionsThere was a negative correlation between DO–C2 and Dsubaxial lordosis angles. This suggests that decrease of mid-to lower-cervical lordosis acts as a compensatory mechanism for lordotic correction between the occiput and C2. In occipito-cervical fusion surgery, care must be taken to avoid excessive O–C2 angle correction because it might induce mid-to-lower cervical compensatory decrease of lordosis
Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach
How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging–related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting. Among the screened proteins, we selected α-crystallin B-subunit (αB-crystallin) and peripherin for further investigation because these proteins were previously reported to be related to central nervous system pathologies. Immunohistochemistry and Western blotting revealed significant upregulation of αB-crystallin and peripherin expression in aged rat spinal cord. Further exploration is needed to elucidate the precise mechanism and potential role of these upregulated proteins in spinal cord aging processes
- …