In vitro modelling of epithelial and stromal interactions in non-malignant and malignant prostates

To study the effects of stromal epithelial cell interactions on prostate cancer metastasis, we have used primary human prostatic stromal cells derived from malignant and non-malignant tissues and established epithelial cell lines from normal (PNT1a and PNT2-C2) and tumour (PC-3, DU145 and LNCaP) origins. The effects of stromal cells on epithelial cell growth were studied in direct and indirect (using culture inserts) co-culture and by exposure to stromal cell-conditioned medium (assessed by MTT assay). The influence of stromal cells on epithelial cell invasion was measured using matrigel invasion chambers and on epithelial cell motility using time lapse microscopy. Results indicated that epithelial cell line growth was similarly unaffected or inhibited by stromal cells derived from malignant (n = 8) or non-malignant tissue (n = 8). In contrast, PNT2-C2 and PC-3 cells were found to be the least and the most invasive and motile epithelia respectively. Stromal cultures enhanced the invasion of both epithelial cells, but no differences were observed between the use of malignant and non-malignant tissues. All stromal cultures modestly stimulated PNT2-C2 motility but displayed a greater stimulation of PC-3 cell motility, while stromal cells derived from malignant tissue stimulated PNT2-C2 and PC-3 cell motility more than stromal cultures from non-malignant tissues. © 2000 Cancer Research Campaig

On the precise connection between the GRW master-equation and master-equations for the description of decoherence

We point out that the celebrated GRW master-equation is invariant under translations, reflecting the homogeneity of space, thus providing a particular realization of a general class of translation-covariant Markovian master-equations. Such master-equations are typically used for the description of decoherence due to momentum transfers between system and environment. Building on this analogy we show the exact relationship between the GRW master-equation and decoherence master-equations, further providing a collisional decoherence model formally equivalent to the GRW master-equation. This allows for a direct comparison of order of magnitudes of relevant parameters. This formal analogy should not lead to confusion on the utterly different spirit of the two research fields, in particular it has to be stressed that the decoherence approach does not lead to a solution of the measurement problem. Building on this analogy however the feasibility of the extension of spontaneous localization models in order to avoid the infinite energy growth is discussed. Apart from a particular case considered in the paper, it appears that the amplification mechanism is generally spoiled by such modifications.Comment: 9 pages, latex, no figures, to appear on J. Phys.

Anaemia and blood transfusion in African children presenting to hospital with severe febrile illness

BACKGROUND: Severe anaemia in children is a leading cause of hospital admission and a major cause of mortality in sub-Saharan Africa, yet there are limited published data on blood transfusion in this vulnerable group. METHODS: We present data from a large controlled trial of fluid resuscitation (Fluid Expansion As Supportive Therapy (FEAST) trial) on the prevalence, clinical features, and transfusion management of anaemia in children presenting to hospitals in three East African countries with serious febrile illness (predominantly malaria and/or sepsis) and impaired peripheral perfusion. RESULTS: Of 3,170 children in the FEAST trial, 3,082 (97%) had baseline haemoglobin (Hb) measurement, 2,346/3,082 (76%) were anaemic (Hb <10 g/dL), and 33% severely anaemic (Hb <5 g/dL). Prevalence of severe anaemia varied from 12% in Kenya to 41% in eastern Uganda. 1,387/3,082 (45%) children were transfused (81% within 8 hours). Adherence to WHO transfusion guidelines was poor. Among severely anaemic children who were not transfused, 52% (54/103) died within 8 hours, and 90% of these deaths occurred within 2.5 hours of randomisation. By 24 hours, 128/1,002 (13%) severely anaemic children had died, compared to 36/501 (7%) and 71/843 (8%) of those with moderate and mild anaemia, respectively. Among children without severe hypotension who were randomised to receive fluid boluses of 0.9% saline or albumin, mortality was increased (10.6% and 10.5%, respectively) compared to controls (7.2%), regardless of admission Hb level. Repeat transfusion varied from ≤2% in Kenya/Tanzania to 6 to 13% at the four Ugandan centres. Adverse reactions to blood were rare (0.4%). CONCLUSIONS: Severe anaemia complicates one third of childhood admissions with serious febrile illness to hospitals in East Africa, and is associated with increased mortality. A high proportion of deaths occurred within 2.5 hours of admission, emphasizing the need for rapid recognition and prompt blood transfusion. Adherence to current WHO transfusion guidelines was poor. The high rates of re-transfusion suggest that 20 mL/kg whole blood or 10 mL/kg packed cells may undertreat a significant proportion of anaemic children. Future evaluation of the impact of a larger volume of transfused blood and optimum transfusion management of children with Hb of <6 g/dL is warranted. Please see related article: http://dx.doi.org/10.1186/s12916-014-0248-5. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12916-014-0246-7) contains supplementary material, which is available to authorized users

Time Dependent Theory for Random Lasers

A model to simulate the phenomenon of random lasing is presented. It couples Maxwell's equations with the rate equations of electronic population in a disordered system. Finite difference time domain methods are used to obtain the field pattern and the spectra of localized lasing modes inside the system. A critical pumping rate $P_{r}^{c}$ exists for the appearance of the lasing peaks. The number of lasing modes increase with the pumping rate and the length of the system. There is a lasing mode repulsion. This property leads to a saturation of the number of modes for a given size system and a relation between the localization length $\xi$ and average mode length $L_m$.Comment: 8 pages. Send to PR

On the solutions of the Schrodinger equation with some molecular potentials: wave function ansatz

Making an ansatz to the wave function, the exact solutions of the $D$% -dimensional radial Schrodinger equation with some molecular potentials like pseudoharmonic and modified Kratzer potentials are obtained. The restriction on the parameters of the given potential, $\delta$ and $\eta$ are also given, where $\eta$ depends on a linear combination of the angular momentum quantum number $\ell$ and the spatial dimensions $D$ and $\delta$ is a parameter in the ansatz to the wave function. On inserting D=3, we find that the bound state eigensolutions recover their standard analytical forms in literature.Comment: 14 page

Supersymmetry and the relationship between a class of singular potentials in arbitrary dimensions

The eigenvalues of the potentials $V_{1}(r)=\frac{A_{1}}{r}+\frac{A_{2}}{r^{2}}+\frac{A_{3}}{r^{3}}+\frac{A_{4 }}{r^{4}}$ and $V_{2}(r)=B_{1}r^{2}+\frac{B_{2}}{r^{2}}+\frac{B_{3}}{r^{4}}+\frac{B_{4}}{r^ {6}}$, and of the special cases of these potentials such as the Kratzer and Goldman-Krivchenkov potentials, are obtained in N-dimensional space. The explicit dependence of these potentials in higher-dimensional space is discussed, which have not been previously covered.Comment: 13 pages article in LaTEX (uses standard article.sty). Please check "http://www1.gantep.edu.tr/~ozer" for other studies of Nuclear Physics Group at University of Gaziante

Theory of decoherence in a matter wave Talbot-Lau interferometer

We present a theoretical framework to describe the effects of decoherence on matter waves in Talbot-Lau interferometry. Using a Wigner description of the stationary beam the loss of interference contrast can be calculated in closed form. The formulation includes both the decohering coupling to the environment and the coherent interaction with the grating walls. It facilitates the quantitative distinction of genuine quantum interference from the expectations of classical mechanics. We provide realistic microscopic descriptions of the experimentally relevant interactions in terms of the bulk properties of the particles and show that the treatment is equivalent to solving the corresponding master equation in paraxial approximation.Comment: 20 pages, 4 figures (minor corrections; now in two-column format

Collisional decoherence observed in matter wave interferometry

We study the loss of spatial coherence in the extended wave function of fullerenes due to collisions with background gases. From the gradual suppression of quantum interference with increasing gas pressure we are able to support quantitatively both the predictions of decoherence theory and our picture of the interaction process. We thus explore the practical limits of matter wave interferometry at finite gas pressures and estimate the required experimental vacuum conditions for interferometry with even larger objects.Comment: 4 pages, 3 figure

WHO guidelines on fluid resuscitation in children: missing the FEAST data.

The World Health Organization recommendations on management of common childhood illnesses affect the lives of millions of children admitted to hospital worldwide. Its latest guidelines,1 released in May 2013, continue to recommend rapid fluid resuscitation for septic shock, even though the only large controlled trial of this treatment (Fluid Expansion as a Supportive Treatment (FEAST) found that it increased the risk of death in African children.2 A subsequent systematic review of bolus resuscitation in children with shock resulting from severe infection also did not support its use.3 Failure to take this evidence into account is not consistent with WHO’s commitment to systematically and transparently assess evidence using the GRADE (Grading of Recommendations Assessment, Development and Evaluation) process when producing guidelines and could endanger the lives of children