Colloidal Stability of Chitosan/DNA Polyelectrolyte Complexes in Presence of Biological Polyanions

International audienceNatural or synthetic polycations are used in nucleic acid‐based therapies as complexing agents which interact electrostatically with nucleic acids, condense them into nanoparticles, protect them and control their entry into cells. However, although the literature on the formation of nanoparticles known as complexes is well documented, fewer studies have focused on the physical chemistry behind their disassembly, especially under physicochemical conditions found in an intracellular environment. There are several theories of the disassembly of these complexes, one of them consisting in the exchange between the polycations of these particles with biological polyanions. This project is focused on the study of the complexation mechanism of chitosan and calf‐thymus DNA, as well as the stability of the obtained complexes in presence of biological polyanions, i.e., glycosaminoglycans (GAGs). In the presence of polyelectrolyte complexes, GAGs that are present in cells are expected to compete with nucleic acids and dissociate the complex if polycation–GAG association is thermodynamically favored. It is found that chitosan/DNA complexes colloidal stability depends on its [N+]/[P−] charge ratio (R). Furthermore, it is determined that the aggregation onset of the complexes, generated by the addition of different GAGs, depends on the structure and the charge density of the GAGs

Physicomechanical and Morphological Characterization of Multi-Structured Potassium-Acrylate-Based Hydrogels

In this work, a photo-polymerization route was used to obtain potassium acrylate-co-acrylamide hydrogels with enhanced mechanical properties, well-defined microstructures in the dry state, and unique meso- and macrostructures in the hydrated state. The properties of the hydrogels depended on the concentration of the crosslinking agent. Mechanical properties, swelling capacity, and morphology were analyzed, showing a well-defined transition at a critical concentration of the crosslinker. In terms of morphology, shape-evolving surface patterns appeared at different scales during swelling. These surface structures had a noticeable influence on the mechanical properties. Hydrogels with structures exhibited better mechanical properties compared to unstructured hydrogels. The critical crosslinking concentration reported in this work (using glycerol diacrylate) is a reference point for the future preparation of multistructured acrylic hydrogel with enhanced properties