09. Matching Long-Term Fire Effects Research to Pressing Questions Facing Tallgrass Prairie Managers across the Upper Midwest

The goal for this paper is to explore how a network of coordinated prescribed fire experiments could be developed and applied to tallgrass prairie management. In a 2011 survey conducted by the Tallgrass Prairie and Oak Savanna Fire Science Consortium in their region, 61% of 207 land managers indicated that their greatest need with respect to fire regimes was information on the outcome of variations in fire frequency and season, with information on these variables ranging from limited to completely lacking. Need for this kind of information was echoed during a breakout discussion session at the 2016 North American Prairie Conference where researchers and land managers shared their opinions on how the potential costs and benefits of developing a research network with experimental treatments could be relevant to management needs. The discussion was encouraging, although researchers noted funding as an important barrier. An example of the informative nature of long-term fire studies is ongoing at the University of Nebraska at Omaha where an experiment established in 1978 has shown strong differences among vegetation and soils in plots burned in different seasons and with different frequencies. A network of sites replicating this type of experiment across the region would inform land management decisions at a broad array of sites that are represented by a variety of soils, weather, climate, and plant species, including invasive plants. All these variables have been hypothesized to be important predictors of fire effects at some location, but the relative importance of different variables across the region has not been quantified through monitoring or research. In this paper, we outline potential steps for a sustained effort to investigate the benefits and risks of engaging in and funding a regional fire research network

Mobile Stress Recognition and Relaxation Support with SmartCoping: User-Adaptive Interpretation of Physiological Stress Parameters

The paper describes a mobile solution for the early recognition and management of stress based on continuous monitoring of heart rate variability (HRV) and contextual data (activity, location, etc.). A central contribution is the automatic calibration of measured HRV values to perceived stress levels during an initial learning phase where the user provides feedback when prompted by the system. This is crucial as HRV varies greatly among people. A data mining component identifies recurrent stress situations so that people can develop appropriate stress avoidance and coping strategies. A biofeedback component based on breathing exercises helps users relax. The solution is being tested by healthy volunteers before conducting a clinical study with patients after alcohol detoxification

Isolation of Whole Esophageal Gland Cells from Plant-Parasitic Nematodes for Transcriptome Analyses and Effector Identification

Esophageal glands of plant-parasitic nematodes are highly specialized cells whose gene expression products include secreted effector proteins, which govern nematode parasitism of host plants. Therefore, elucidating the transcriptomes of esophageal glands with the goal of identifying nematode effectors is a promising avenue to understanding nematode parasitism and its evolutionary origins as well as to devising nematode control strategies. We have developed a method to separate and isolate individual esophageal gland cells from multiple species of plant-parasitic nematodes while preserving RNA quality. We have used such isolated gland cells for transcriptome analysis via high-throughput DNA sequencing. This method relies on the differential histochemical staining of the gland cells after homogenization of phytonematode tissues. Total RNA was extracted from whole gland cells isolated from eight different plant-parasitic nematode species. To validate this approach, the isolated RNA from three plant-parasitic nematode species—Globodera rostochiensis, Pratylenchus penetrans, and Radopholus similis—was amplified, gel purified, and used for 454 sequencing. We obtained 456,801 total reads with an average read length of 409 bp. Sequence analyses revealed the presence of homologs of previously known nematode effectors in these libraries, thus validating our approach. These data provide compelling evidence that this technical advance can be used to relatively easily and expediently discover effector repertoires of plant-parasitic nematodes

GmEREBP1 Is a Transcription Factor Activating Defense Genes in Soybean and Arabidopsis

Ethylene-responsive element-binding proteins (EREBPs) are plant-specific transcription factors, many of which have been linked to plant defense responses. Conserved EREBP domains bind to the GCC box, a promoter element found in pathogenesis-related (PR) genes. We previously identified an EREBP gene from soybean (GmEREBP1) whose transcript abundance decreased in soybean cyst-nematode-infected roots of a susceptible cultivar, whereas it increased in abundance in infected roots of a resistant cultivar. Here, we report further characterization of this gene. Transient expression analyses showed that GmEREBP1 is localized to the plant nucleus and functions as a transcriptional activator in soybean leaves. Transgenic soybean plants expressing GmEREBP1 activated the expression of the ethylene (ET)-responsive gene PR2 and the ET- and jasmonic acid (JA)-responsive gene PR3, and the salicylic acid (SA)-responsive gene PR1 but not the SA-responsive PR5. Similarly, transgenic Arabidopsis plants expressing GmEREBP1 showed elevated mRNA abundance of the ET-regulated gene PR3 and the ET- and JA-regulated defense-related gene PDF1.2 but not the ET-regulated GST2, and the SA-regulated gene PR1 but not the SA-regulated PR2 and PR5. Transgenic soybean and Arabidopsis plants inoculated with cyst nematodes did not display a significantly altered susceptibility to nematode infection. These results collectively show that GmEREBP1 functions as a transacting inducer of defense gene expression in both soybean and Arabidopsis and mediates the expression of both ET- and JA- and SA-regulated defense-related genes in these plant species